Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,571,677	C→G	5.3%	R236G (CGC→GGC)	yneJ →	LysR family DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,571,677	0	C	G	5.3%	246.0 / 3.8	75	R236G (CGC→GGC)	yneJ	LysR family DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (36/35);  new base G (2/2);  total (38/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.08e-01

CGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCT  >  NC_002947/3571536‑3571816                                                                                                                                              |                                                                                                                                             cgcgCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCAt                                                                                                                                       <  1:28307/149‑1 (MQ=255) cgcgCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCAt                                                                                                                                       <  7:334686/149‑1 (MQ=255)     ccAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTc                                                                                                                                   <  3:270503/149‑1 (MQ=255)            ttCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACaa                                                                                                                              <  4:371153/147‑1 (MQ=255)            ttCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAata                                                                                                                            >  3:226860/1‑149 (MQ=255)            ttCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAat                                                                                                                             <  4:152353/148‑1 (MQ=255)              cATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATg                                                                                                                          >  3:192090/1‑149 (MQ=255)                      cATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGTTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTg                                                                                                                  <  3:299893/149‑1 (MQ=255)                        tCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCAt                                                                                                                                       <  5:282495/126‑1 (MQ=255)                        tCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCGCGGCAGCAt                                                                                                                                       >  6:282495/1‑126 (MQ=255)                          aCTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTgcag                                                                                                               >  1:109623/1‑148 (MQ=255)                            ttCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CAc                                                                                                           >  1:334643/1‑149 (MQ=255)                              cGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCg                                                                                                         >  2:37955/1‑149 (MQ=255)                               gAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGt                                                                                                        >  2:284838/1‑149 (MQ=255)                                aaaaCTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgcagc                                                                                                                                         >  7:96808/1‑116 (MQ=255)                                aaaaCTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCgcagc                                                                                                                                         <  8:96808/116‑1 (MQ=255)                                 aaaCTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGt                                                                                                        >  7:157309/1‑147 (MQ=255)                                       gTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCt                                                                                                >  2:136083/1‑149 (MQ=255)                                        ttCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTg                                                                                               <  4:226860/149‑1 (MQ=255)                                                  aCCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCg                                                                                     <  5:175541/149‑1 (MQ=255)                                                    cAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCgga                                                                                   <  8:157309/149‑1 (MQ=255)                                                    cAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCgga                                                                                   <  2:109623/149‑1 (MQ=255)                                                      ggCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCggagg                                                                                 <  2:334643/149‑1 (MQ=255)                                                      ggCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCggag                                                                                  >  5:22634/1‑148 (MQ=255)                                                      ggCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCggag                                                                                  >  5:116173/1‑148 (MQ=255)                                                             ccGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCg                                                                          >  8:315876/1‑149 (MQ=255)                                                              cGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGc                                                                         >  1:18643/1‑149 (MQ=255)                                                                ggCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTa                                                                       <  1:37955/149‑1 (MQ=255)                                                                 gCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTAt                                                                      >  7:145209/1‑149 (MQ=255)                                                                    aGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTg                                                                   <  7:257064/149‑1 (MQ=255)                                                                          aCGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAcc                                                             >  2:106853/1‑149 (MQ=255)                                                                          aCGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAcc                                                             >  3:361977/1‑149 (MQ=255)                                                                          aCGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAAcc                                                             >  8:199844/1‑149 (MQ=255)                                                                            gAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctg                                                           >  3:344892/1‑149 (MQ=255)                                                                               aTGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCATGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggct                                                        >  7:134699/1‑149 (MQ=255)                                                                                  gAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGt                                                     <  4:361977/149‑1 (MQ=255)                                                                                  gAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGt                                                     <  7:199844/149‑1 (MQ=255)                                                                                   aGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTa                                                    <  1:284838/149‑1 (MQ=255)                                                                                    gTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTAt                                                   <  2:18643/149‑1 (MQ=255)                                                                                     tCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATg                                                  <  6:22634/149‑1 (MQ=255)                                                                                       gTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggctg                                                       <  6:230739/142‑1 (MQ=255)                                                                                       gTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACctggctg                                                       >  5:230739/1‑142 (MQ=255)                                                                                       gTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGc                                                >  1:122699/1‑149 (MQ=255)                                                                                        tACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGc                                                >  4:6536/1‑148 (MQ=255)                                                                                          ccACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGcga                                              <  2:122699/148‑1 (MQ=255)                                                                                             cGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAg                                          <  4:344892/149‑1 (MQ=255)                                                                                              ggCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAgg                                         >  6:192569/1‑149 (MQ=255)                                                                                               gCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGt                                        <  6:116173/149‑1 (MQ=255)                                                                                                  tGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACg                                     <  1:136083/149‑1 (MQ=255)                                                                                                   gTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACgg                                    >  4:339993/1‑149 (MQ=255)                                                                                                    ttGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGt                                   >  5:143171/1‑149 (MQ=255)                                                                                                       gCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGtctc                                >  1:5588/1‑149 (MQ=255)                                                                                                         cTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACct                                                            >  7:134594/1‑119 (MQ=255)                                                                                                         cTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACct                                                            <  8:134594/119‑1 (MQ=255)                                                                                                         cTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTcgc                              <  8:145209/149‑1 (MQ=255)                                                                                                              tCAGTGCGGGTGCCGGCCTGGCCATGCTTCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTAACTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGt                         <  1:260597/149‑1 (MQ=255)                                                                                                               cAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACg                                     <  8:252179/136‑1 (MQ=255)                                                                                                               cAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACg                                     >  7:252179/1‑136 (MQ=255)                                                                                                               cAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCa                           >  1:3280/1‑146 (MQ=255)                                                                                                               cAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCa                           <  2:3280/146‑1 (MQ=255)                                                                                                                 gTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCt                                                                                                >  7:95476/1‑75 (MQ=255)                                                                                                                 gTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCt                                                                                                <  8:95476/75‑1 (MQ=255)                                                                                                                    cGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGc                   <  7:315876/149‑1 (MQ=255)                                                                                                                        tGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGt               >  5:70354/1‑149 (MQ=255)                                                                                                                          ccGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTc             <  3:6536/149‑1 (MQ=255)                                                                                                                           cGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAGTCACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACGTGGCTGGTATGGCGACGAGGTCCGGTCTCGCGCAGTTTGAGCATGTTc             >  1:92272/1‑149 (MQ=255)                                                                                                                           cGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGc                   <  5:105391/142‑1 (MQ=255)                                                                                                                           cGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGc                   >  6:105391/1‑142 (MQ=255)                                                                                                                             gCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGt           >  6:79738/1‑148 (MQ=255)                                                                                                                                 gagcATGCTGCGCGGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTc                                                                            >  5:113451/4‑79 (MQ=38)                                                                                                                                 gagcATGCTGCGCGGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTc                                                                            <  6:113451/76‑1 (MQ=38)                                                                                                                                  gCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGtt     >  7:294235/1‑149 (MQ=255)                                                                                                                                   ccATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTg    >  6:338853/1‑149 (MQ=255)                                                                                                                                    cATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGc   <  5:79738/149‑1 (MQ=255)                                                                                                                                     aTGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCt  <  3:339993/149‑1 (MQ=255)                                                                                                                                              |                                                                                                                                             CGCGCCAACTGTTCATACCGCCATCACTTCGAAAACTGGTTCGTGCAGGACCAGGCGGTACCGGGCAAGATTCACGAAATGGAGTCGTACCACGGCATGTTGGCCTGCGTCAGTGCGGGTGCCGGCCTGGCCATGCTGCCGCGCAGCATGCTCGACAATATGCCCGGGTGCAG‑CACCGTCAGCGCCTGGCCAATGTCGGAGGACTTCCGCTATCTGAAAACCTGGCTGGTATGGCGACGAGGTACGGTCTCGCGCAGTTTGAGCATGTTCGTGAAGTTGCT  >  NC_002947/3571536‑3571816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑