Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,579,071 T→A 7.6% F142Y (TTT→TAT) ‡ PP_5589 → rhs repeat‑associated core domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,579,0710TA 7.6% 202.7 / 5.1 66F142Y (TTT→TAT) ‡PP_5589rhs repeat‑associated core domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (29/32);  new base A (3/2);  total (32/34)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

ACCGCCGCAGGATATAATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAA  >  NC_002947/3578928‑3579217
                                                                                                                                               |                                                                                                                                                  
aCCGCCGCAGGATATAATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATc                                                                                                                                                >  5:3045/1‑148 (MQ=255)
  cgccgcAGGATATAATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAg                                                                                                                                             >  7:39184/1‑149 (MQ=255)
   gcctcATGATATAATTCAGTCCAGAGCTTAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTTACTGTGCTTGCTCGCTTGAGGTGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGTATCTGGAAGTGCTTGATTTTATCaatc                                                                                                                                            <  4:117124/149‑3 (MQ=255)
      gcAGGATATAATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATAAAATCAAGcact                                                                                                                                         <  8:13677/149‑4 (MQ=255)
        aGGATATAATTGAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGTACGTAATTCGTAGTTGACTGTGCTTGCTCGCTTTAGGGGGTCGCCTTCTTCTAGCAGAAATATATTACAGGAGGATCTGGAAGTGCTTGATTTTATCAAGCgtgt                                                                                                                                        <  1:18928/148‑1 (MQ=255)
          gATATAATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGtgttgt                                                                                                                                     >  8:235157/1‑149 (MQ=255)
            tataATTCAGTCCAGCCCTGAGTTAAGGCC‑TTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGc                                                                                                                                  >  7:323833/1‑149 (MQ=255)
             ataATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGc                                                                                                                                  >  4:60005/1‑149 (MQ=255)
                 ttCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCcct                                                                                                                              >  2:335659/1‑149 (MQ=255)
                   cAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCcctc                                                                                                                             <  3:60005/148‑1 (MQ=255)
                      tCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTg                                                                                                                          >  7:35647/1‑148 (MQ=255)
                      tCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGt                                                                                                                         >  2:306013/1‑149 (MQ=255)
                             cTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACggg                                                                                                                  <  6:3045/149‑1 (MQ=255)
                                aGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACgggg                                                                                                                 <  3:27101/147‑1 (MQ=255)
                                aGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCCGTCGACGGGggt                                                                                                               >  8:318190/1‑149 (MQ=255)
                                 gTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGggtg                                                                                                              <  3:49295/149‑1 (MQ=255)
                                 gTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGggtg                                                                                                              <  7:41773/149‑1 (MQ=255)
                                  ttAAGGCCTTTTGTATAACGTAATTCGAAGTTGACTGTGCTTGCGCGCTTGCGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGggtg                                                                                                              <  3:247159/148‑1 (MQ=255)
                                  ttAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCCGTCGACGGGggtgg                                                                                                             <  7:318190/149‑1 (MQ=255)
                                    aaGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGggtggt                                                                                                            <  8:35647/148‑1 (MQ=255)
                                       gCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCcctc                                                                                                                             >  5:120654/1‑128 (MQ=255)
                                       gCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCcctc                                                                                                                             <  6:120654/128‑1 (MQ=255)
                                        ccTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGgtggtg                                                                                                           >  4:320238/1‑145 (MQ=255)
                                         cTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGTGGGTGGTGTAgat                                                                                                      <  5:22547/149‑1 (MQ=255)
                                          ttttGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAg                                                                                                                                             <  2:176235/109‑1 (MQ=255)
                                          ttttGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAgat                                                                                                      >  3:217101/1‑148 (MQ=255)
                                                aGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTCTCGACGGGGGTGGTGTATATTAttgtt                                                                                               >  4:577/1‑149 (MQ=255)
                                                  aaCGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGAttgtttg                                                                                             >  6:54490/1‑149 (MQ=255)
                                                        aTTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTTTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTtgct                                                                                       <  1:80849/149‑1 (MQ=255)
                                                         ttCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACggggg                                                                                                                >  3:33715/1‑123 (MQ=255)
                                                         ttCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACggggg                                                                                                                <  4:33715/123‑1 (MQ=255)
                                                         ttCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATAAAATCAAGcactt                                                                                                                                        >  5:266681/1‑95 (MQ=255)
                                                         ttCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATAAAATCAAGcactt                                                                                                                                        <  6:266681/99‑5 (MQ=255)
                                                                   aCTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTa                                                                             <  8:87025/148‑1 (MQ=255)
                                                                     tgtgCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGt                                                                           >  4:102182/1‑148 (MQ=255)
                                                                          ttgctCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATAtt                                                                     >  7:51295/1‑149 (MQ=255)
                                                                            gctCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGAttgtttgt                                                                                            >  4:215759/1‑124 (MQ=255)
                                                                            gctCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGAttgtttgt                                                                                            <  3:215759/124‑1 (MQ=255)
                                                                             ctCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGc                                                                   <  3:320238/148‑1 (MQ=255)
                                                                              tCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATCAATGTTTGTTTCCTGCTTATTCTAGAAATATTGcc                                                                  >  8:184732/1‑148 (MQ=255)
                                                                                  ttGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTgg                                                             <  3:356494/149‑1 (MQ=255)
                                                                                                   aTCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCt                                             <  8:51295/148‑1 (MQ=255)
                                                                                                      tAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGGTAGAATTCCTTAGt                                         >  7:295267/1‑149 (MQ=255)
                                                                                                       aGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGt                                         <  8:39184/148‑1 (MQ=255)
                                                                                                             aaTATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTtgatga                                   <  4:217101/148‑1 (MQ=255)
                                                                                                                   ttACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTAc                            <  1:306013/149‑1 (MQ=255)
                                                                                                                            gaTCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTa                             >  3:38378/1‑139 (MQ=255)
                                                                                                                            gaTCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTa                             <  4:38378/139‑1 (MQ=255)
                                                                                                                              tCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGaaa                                 >  1:323014/1‑133 (MQ=255)
                                                                                                                              tCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGaaa                                 <  2:323014/133‑1 (MQ=255)
                                                                                                                              tCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGAt                   >  6:5436/1‑147 (MQ=255)
                                                                                                                                 ggAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGt                               >  4:305675/1‑132 (MQ=255)
                                                                                                                                 ggAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGt                               <  3:305675/132‑1 (MQ=255)
                                                                                                                                  gAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAggg              <  3:102182/148‑1 (MQ=255)
                                                                                                                                  gAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGc             >  8:118256/1‑149 (MQ=255)
                                                                                                                                   aaGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCt            <  5:54490/149‑1 (MQ=255)
                                                                                                                                    cacgCTTGATAAAATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCt            >  2:33242/4‑148 (MQ=255)
                                                                                                                                    cacgCTTGATAAAATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCt            >  2:27642/4‑148 (MQ=255)
                                                                                                                                     gTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAg                                                    >  2:108141/1‑107 (MQ=255)
                                                                                                                                     gTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAg                                                    <  1:108141/107‑1 (MQ=255)
                                                                                                                                        cTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTg                      >  4:252037/1‑134 (MQ=255)
                                                                                                                                        cTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTg                      <  3:252037/134‑1 (MQ=255)
                                                                                                                                        cTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACg       >  4:169844/1‑149 (MQ=255)
                                                                                                                                         ttGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGAtt                  >  5:234396/1‑137 (MQ=255)
                                                                                                                                         ttGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGAtt                  <  6:234396/137‑1 (MQ=255)
                                                                                                                                           gATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGt      <  8:323833/147‑1 (MQ=255)
                                                                                                                                             ttttATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGaaa  >  2:102565/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
ACCGCCGCAGGATATAATTCAGTCCAGCCCTGAGTTAAGGCCTTTTGTAGAACGTAATTCGAAGTTGACTGTGCTTGCTCGCTTGAGGGGGTCGCCTTCATCTAGCAGAAATATATTACCGGAGGATCTGGAAGTGCTTGATTTTATCAAGCGTGTTGTCGCCCCTCCTGTCGACGGGGGTGGTGTAGATGATTGTTTGTTTGCTGCTTATGCTAGTAATATTGCCTCTGGCAGCGCTAGAATTCCTGAGTTGATGAAAGTTACTGCTTGGATTAGGGCTGAACGTGAAA  >  NC_002947/3578928‑3579217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑