Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,630,323 G→A 13.0% W315* (TGG→TAG) ‡ PP_3200 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,630,3230GA13.0% 92.5 / 4.4 38W315* (TGG→TAG) ‡PP_3200hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (18/15);  new base A (3/2);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATC  >  NC_002947/3630175‑3630468
                                                                                                                                                    |                                                                                                                                                 
tATTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTg                                                                                                                                                   >  8:281921/1‑149 (MQ=255)
 aTTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTgg                                                                                                                                                  >  1:193990/1‑149 (MQ=255)
      tcCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCAc                                                                                                                                             >  6:116615/1‑149 (MQ=255)
       ccACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCAcc                                                                                                                                            >  7:181525/1‑149 (MQ=255)
            gacgacTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGAc                                                                                                                                       <  2:300677/149‑1 (MQ=255)
                     tttGGCGATGGCGCCGAGCGCATGTTCGTCGCCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGCGCAGGGCGGCATGCAGGTGCGCTGCCGCACCGAGACCGTCGACTg                                                                                                                              >  4:177525/1‑149 (MQ=255)
                       tGGCGTTGGCGCCGAGCGGATGTTCGTAGGCGCGCCGGTGTTCCCCAGTGGTCAACCTCTGGCGTTGTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACt                                                                                                                               <  2:142070/146‑1 (MQ=255)
                        ggCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTggg                                                                                                                            <  5:210641/148‑1 (MQ=255)
                             tGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTg                                                                                                                       >  5:257727/1‑148 (MQ=255)
                                                   ggCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACaa                                                                                                <  6:341791/149‑1 (MQ=255)
                                                             tgtTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCaa                                                                                      <  7:281921/149‑1 (MQ=255)
                                                                                 ctGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACtt                                                                  >  4:281729/1‑149 (MQ=255)
                                                                                  tGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACgg                                                                                                                                     >  6:96409/1‑81 (MQ=255)
                                                                                  tGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACgg                                                                                                                                     <  5:96409/81‑1 (MQ=255)
                                                                                    gCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTc                                                                 <  6:257727/147‑1 (MQ=255)
                                                                                          ttcCACGGCAACGACAAGCAAGTCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGc                                                                          >  7:57795/1‑132 (MQ=255)
                                                                                          ttcCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGAGTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCt                                                         >  1:117279/1‑149 (MQ=255)
                                                                                              aCGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGAc                                                     >  2:19503/1‑149 (MQ=255)
                                                                                                         aagcaagcCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCgg                                          >  1:235921/1‑149 (MQ=255)
                                                                                                                 cAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCg                                  >  8:14127/1‑149 (MQ=255)
                                                                                                                     gACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGc                                >  5:254639/1‑147 (MQ=255)
                                                                                                                        tCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGtt                           >  4:69985/1‑149 (MQ=255)
                                                                                                                          agggcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGtt                           <  5:116615/147‑1 (MQ=255)
                                                                                                                          agggcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGtt                           <  6:254639/147‑1 (MQ=255)
                                                                                                                          agggcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGtt                           <  2:117279/147‑1 (MQ=255)
                                                                                                                          agggcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTc                          >  5:6217/1‑148 (MQ=255)
                                                                                                                          agggcagggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTc                          <  3:360990/148‑1 (MQ=255)
                                                                                                                               agggcGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTgg                    <  1:853/149‑1 (MQ=255)
                                                                                                                                 ggcggcATGCAGGTGCGCTGGCGCAACGAGACCGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTAATCGGCAAGGCCGGCGAGTTCTACTGGgt                  >  8:92046/1‑149 (MQ=255)
                                                                                                                                   cggcATGCAGGTGCGTTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAg                                             <  4:204273/120‑1 (MQ=255)
                                                                                                                                   cggcATGCAGGTGCGTTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAg                                             >  3:204273/1‑120 (MQ=255)
                                                                                                                                          tcggTGCGCCAGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATccc           >  6:239110/3‑147 (MQ=255)
                                                                                                                                           cggTGCGCCAGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTc                          <  2:295006/130‑1 (MQ=255)
                                                                                                                                           cggTGCGCCAGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTc                          <  2:290680/130‑1 (MQ=255)
                                                                                                                                           cggTGCGCCAGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTc                          >  1:295006/2‑131 (MQ=255)
                                                                                                                                           cggTGCGCCAGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTc                          >  1:290680/2‑131 (MQ=255)
                                                                                                                                            ggggcgcTGGCGCACCGAGACGGTCGACTGGGGCGTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCTGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAgg       <  7:171084/146‑1 (MQ=255)
                                                                                                                                             gTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCgg                                 <  2:67744/122‑1 (MQ=255)
                                                                                                                                             gTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCgg                                 >  1:67744/1‑122 (MQ=255)
                                                                                                                                                 gcTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATc  >  6:117074/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
TATTTCTCCACAGACGACTTCTTTGGCGATGGCGCCGAGCGCATGTTCGTCGGCGCGCCGGTGTTCCCCAGTGGCCAACCTCTGGCGTTCTTCCACGGCAACGACAAGCAAGCCAAGGACTCAGGGCAGGGCGGCATGCAGGTGCGCTGGCGCACCGAGACGGTCGACTGGGGCCTGTACGCCATCCGTTTCCACGACAAGTCGCCGCAACTCAATGTACGCCCGAACTTCGCCAACCTCGACCCGGTCAGCGGCAAGGCCGGCGAGTTCTACTGGGTGTATCCCGAGGGTATC  >  NC_002947/3630175‑3630468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑