Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,652,702 T→A 7.4% H196Q (CAT→CAA)  PP_3218 → NtaA/SnaA/SoxA family monooxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,652,7020TA 7.4% 160.4 / 3.2 54H196Q (CAT→CAA) PP_3218NtaA/SnaA/SoxA family monooxygenase
Reads supporting (aligned to +/- strand):  ref base T (28/22);  new base A (2/2);  total (30/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

CCGTTACGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCACGCCGCCAGCATCGAG  >  NC_002947/3652558‑3652843
                                                                                                                                                |                                                                                                                                             
ccGTTACGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTtct                                                                                                                                           >  3:194503/1‑149 (MQ=255)
ccGTTACGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTtct                                                                                                                                           >  8:154126/1‑149 (MQ=255)
  gTTACGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCg                                                                                                                                         >  6:216442/1‑149 (MQ=255)
  gTTACGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCg                                                                                                                                         <  2:187094/149‑1 (MQ=255)
      cGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGgtc                                                                                                                                      <  3:174174/148‑1 (MQ=255)
         ccGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGtcgtcg                                                                                                                                  >  1:145763/1‑149 (MQ=255)
                gaggagCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCt                                                                                                                           >  5:326289/1‑149 (MQ=255)
                 aggagCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCt                                                                                                                           >  3:328516/1‑148 (MQ=255)
                       aTGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATc                                                                                                                    <  4:328516/149‑1 (MQ=255)
                             aaGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAgcgc                                                                                                              >  1:27071/1‑149 (MQ=255)
                                   tGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGccc                                                                                                        <  2:265898/149‑1 (MQ=255)
                                     cAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGccc                                                                                                        >  1:126104/1‑147 (MQ=255)
                                      aGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAg                                                                                                     >  4:151130/1‑149 (MQ=255)
                                      aGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAg                                                                                                     <  8:172763/149‑1 (MQ=255)
                                        gggCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAggg                                                                                                   <  3:151130/149‑1 (MQ=255)
                                                gCAGTCGTACGAAGCCGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCgg                                                                                           <  7:336047/149‑1 (MQ=255)
                                                gCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAgggg                                                                                                  <  4:176627/142‑1 (MQ=255)
                                                gCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAgggg                                                                                                  >  3:176627/1‑142 (MQ=255)
                                                      gTACGAAGACGATGCTGTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCACGCTGCATGCGCTCAGCCACACGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACAGCCAGCGCTCGCCCCAGGGGCGGCCGGTGctct                                                                                     >  8:90823/1‑149 (MQ=255)
                                                       tACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCtt                                                                                    >  6:238857/1‑149 (MQ=255)
                                                          gAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCa                                                                                 <  7:154126/149‑1 (MQ=255)
                                                                gATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAggcggg                                                                           <  2:27071/149‑1 (MQ=255)
                                                                  tGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCAATTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCa                                                                                 <  8:289331/141‑1 (MQ=255)
                                                                  tGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCAATTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCa                                                                                 <  8:284082/141‑1 (MQ=255)
                                                                  tGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCAATTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCa                                                                                 >  7:289331/1‑141 (MQ=255)
                                                                  tGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCAATTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCa                                                                                 >  7:284082/1‑141 (MQ=255)
                                                                       tCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTc                                                                     <  4:194503/148‑1 (MQ=255)
                                                                           ccGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTcc                                                                    >  3:190000/1‑145 (MQ=255)
                                                                           ccGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTcc                                                                    <  4:190000/145‑1 (MQ=255)
                                                                           ccGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGaa                                                                 >  1:73027/1‑148 (MQ=255)
                                                                           ccGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAAc                                                                <  5:216442/149‑1 (MQ=255)
                                                                             gTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACaa                                                              <  2:73027/149‑1 (MQ=255)
                                                                                   gggCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGa                                                        <  5:238857/149‑1 (MQ=255)
                                                                                     gCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGTATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGa                                                        <  1:114659/147‑1 (MQ=255)
                                                                                     gCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGa                                                        >  2:114659/1‑147 (MQ=255)
                                                                                         gTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTc                                                  <  2:126104/149‑1 (MQ=255)
                                                                                          tGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCg                                                 >  4:210246/1‑149 (MQ=255)
                                                                                             ccGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCgggg                                              >  8:287094/1‑149 (MQ=255)
                                                                                                    cTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCAc                                       >  6:328276/1‑149 (MQ=255)
                                                                                                     tCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACg                                      >  5:331545/1‑149 (MQ=255)
                                                                                                     tCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACg                                      >  8:100085/1‑149 (MQ=255)
                                                                                                        acccgaccAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCg                                                                                            >  6:320598/1‑92 (MQ=255)
                                                                                                        acccgaccAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCg                                                                                            <  5:320598/92‑1 (MQ=255)
                                                                                                             accAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGACGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGAtgt                              >  3:137755/1‑149 (MQ=255)
                                                                                                                aaGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATgtggt                           <  7:100085/149‑1 (MQ=255)
                                                                                                                      cATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCa                      <  6:326289/148‑1 (MQ=255)
                                                                                                                          cgcTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCAc                 <  7:90823/149‑1 (MQ=255)
                                                                                                                             tCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCAcgcc              >  1:180360/1‑149 (MQ=255)
                                                                                                                                aCCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCAcgccgc            >  6:31960/1‑148 (MQ=255)
                                                                                                                                 ccACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCACGCCGCCa          <  3:210246/149‑1 (MQ=255)
                                                                                                                                   acaAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCACGCCGCCAg         >  4:214516/1‑148 (MQ=255)
                                                                                                                                    caAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGCTTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTCGTGTTCACCCACTCCGCCAGCa       >  7:179871/1‑149 (MQ=255)
                                                                                                                                       gggTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCACGCCGCCAGCATc     >  8:325445/1‑148 (MQ=255)
                                                                                                                                          tAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCACGCCGCCAGCATCGAg  >  5:101637/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                             
CCGTTACGCCCGTGCCGAGGAGCATGTGCAAGTCGTGCAGGGGCTGTGGCAGTCGTACGAAGACGATGCTTTCCCCCGTGATCGGGCCAGTGGCCGGTTCCTCGACCCGACCAAGCTGCATGCGCTCAACCACAAGGGTAAGCATTTCTCGGTCGTCGGCCCGCTCAACATCCAGCGCTCGCCCCAGGGGCAGCCGGTGATCTTCCAGGCGGGCGATTCCGAACAAGGCCGAGCGCTCGGGGCCGCCACGGCTGATGTGGTGTTCACCCACGCCGCCAGCATCGAG  >  NC_002947/3652558‑3652843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑