Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 295,680 T→A 8.9% intergenic (+28/+114) PP_0241 → / ← PP_0242 transporter/TetR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947295,6800TA 8.9% 122.9 / 3.7 45intergenic (+28/+114)PP_0241/PP_0242transporter/TetR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (18/23);  new base A (2/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01

CGGGTATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCA  >  NC_002947/295537‑295825
                                                                                                                                               |                                                                                                                                                   
cGGGTATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCa                                                                                                                                                  <  8:314710/147‑1 (MQ=255)
cGGGTATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAg                                                                                                                                                 <  2:305040/148‑1 (MQ=255)
  ggTATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCAAAGCAGccc                                                                                                                                              <  5:242003/149‑1 (MQ=255)
    tATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCAAAGCAGcc                                                                                                                                               <  3:58490/146‑1 (MQ=255)
    tATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCAAAGCAGcc                                                                                                                                               >  4:58490/1‑146 (MQ=255)
        gTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGcc                                                                                                                                        >  4:296578/1‑149 (MQ=255)
        gTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGcc                                                                                                                                        >  7:81657/1‑149 (MQ=255)
           aCTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCgg                                                                                                                                      <  8:222257/148‑1 (MQ=255)
                       accaccCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTcc                                                                                                                        <  1:299872/148‑1 (MQ=255)
                        cctcccGTTCCGTGCGGGTGCTTGAGTTGCAGGTGGGCAGTTAAGTGATTGCCTTTGTTAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATTTTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCca                                                                                                                      <  6:323704/146‑1 (MQ=38)
                           cccGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAg                                                                                                                   <  6:304906/149‑1 (MQ=255)
                                    tGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGc                                                                                                           >  6:128857/1‑148 (MQ=255)
                                            cTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAg                                                                                                  >  5:278587/1‑149 (MQ=255)
                                               gAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAgg                                                                                                 <  3:296578/147‑1 (MQ=255)
                                               gAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGt                                                                                               <  5:80506/149‑1 (MQ=255)
                                                            gCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGctct                                                                                  <  8:81657/149‑1 (MQ=255)
                                                              agtgaagtgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCCTAGATCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGctct                                                                                  >  1:309024/1‑149 (MQ=255)
                                                              agtgaagtgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCtgt                                                                                <  1:57959/149‑1 (MQ=255)
                                                                tgaagtgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTgg                                                                              >  8:85177/1‑149 (MQ=255)
                                                                  aagtgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCa                                                                                                          <  8:64461/119‑1 (MQ=255)
                                                                  aagtgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCa                                                                                                          >  7:64461/1‑119 (MQ=255)
                                                                   agtgaTTGCCTTTGTTAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGTGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGa                                                                            <  6:31906/148‑1 (MQ=255)
                                                                     tgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCca                                                                                                                      >  6:116605/1‑104 (MQ=255)
                                                                     tgaTTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCca                                                                                                                      <  5:116605/104‑1 (MQ=255)
                                                                             tttGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGcc                                                                 >  7:34092/1‑149 (MQ=255)
                                                                                 tgAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGct                                                                                    >  3:264642/1‑126 (MQ=255)
                                                                                 tgAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGct                                                                                    <  4:264642/126‑1 (MQ=255)
                                                                                     gTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAgg                                                         <  5:128857/149‑1 (MQ=255)
                                                                                       cGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCAAGCCcgctcccaca                                                       <  7:85177/149‑8 (MQ=255)
                                                                                        gACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGgcc                                                      >  6:71000/1‑149 (MQ=255)
                                                                                                  tttctggCCAAGCTGGGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGc                                            <  7:149131/143‑1 (MQ=255)
                                                                                                  tCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGc                                            >  2:154559/1‑149 (MQ=255)
                                                                                                           aaGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGTGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGa                                      <  5:308919/146‑1 (MQ=255)
                                                                                                           aaGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGTGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGa                                      >  6:308919/1‑146 (MQ=255)
                                                                                                            aGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTtc                                  >  5:241889/1‑149 (MQ=255)
                                                                                                              cTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTtctc                                <  5:71000/149‑1 (MQ=255)
                                                                                                                           ggTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCtt                   <  6:278587/149‑1 (MQ=255)
                                                                                                                            gTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTg                  >  7:87505/1‑149 (MQ=255)
                                                                                                                               gATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGc                <  6:27127/148‑1 (MQ=255)
                                                                                                                                 tatTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGc                >  8:211818/1‑146 (MQ=255)
                                                                                                                                   gaagggCTGCAAAGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGa            >  8:329741/4‑148 (MQ=255)
                                                                                                                                     ggggCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAAc         >  8:223081/1‑149 (MQ=255)
                                                                                                                                       ggCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAAcgg       >  5:124926/1‑149 (MQ=255)
                                                                                                                                       ggCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAAcgg       <  8:34092/149‑1 (MQ=255)
                                                                                                                                            ctTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCa  <  7:223081/149‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                   
CGGGTATCGTCACTTCGGTGATTACCACCCGTTCCGTGCGGGAGCTGGAGTTGCAGGTGGGCAGTGAAGTGATTGCCTTTGTGAAGTCGACCGAAGTGTCTATCGCCAAGCTGTGATGCGGCGGGTCGATATTGGGGCTGCTTTGCAGCCCTTCGCCGGCACGCCC‑‑GCTCCCACAGGTACTCGCAAGCTCCAGGGTGCGCGCGGGCTCTGTGGGAGCGGGCTTGCCCGCGAAGGGCCGCCAAGCGGCCCCAGATTTCTCAGCTGGAACGCTTGGCCAGAAACGGCGGCA  >  NC_002947/295537‑295825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑