Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,794,770 A→G 7.4% L562L (TTG→CTG)  fcs ← feruloyl‑CoA‑synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,794,7700AG 7.4% 153.2 / 2.8 54L562L (TTG→CTG) fcsferuloyl‑CoA‑synthetase
Reads supporting (aligned to +/- strand):  ref base A (21/29);  new base G (2/2);  total (23/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01

GCCTCGACCTGAGCGGCGCGCCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCACGGTCCGGCGCG  >  NC_002947/3794622‑3794918
                                                                                                                                                     |                                                                                                                                                    
gcCTCGACCTGAGCGGCGCGCCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCaa                                                                                                                                                      <  6:74267/149‑1 (MQ=255)
gcCTCGACCTGAGCGGCGCGCCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCaa                                                                                                                                                      <  1:68781/149‑1 (MQ=255)
            gcggcgCGCCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTccagcca                                                                                                                                          <  6:235218/149‑1 (MQ=255)
             cggcgCGCCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTccagccag                                                                                                                                         >  5:354717/1‑149 (MQ=255)
                cgcgcCG‑TGCTGCAGCGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGt                                                                                                                                        >  3:351379/1‑147 (MQ=255)
                 gcgcCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAg                                                                                                                                     >  7:186081/1‑149 (MQ=255)
                      gcTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAAc                                                                                                                                <  8:279788/149‑1 (MQ=255)
                             ca‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTgc                                                                                                                         <  6:69292/149‑1 (MQ=255)
                                      gcTGATTGATCGAGCCCTTGTCGGTTATTTCACCGGCGTCGATCGAGGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGgtg                                                                                                                 <  5:158680/149‑1 (MQ=255)
                                          attgatCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACg                                                                                                                    <  1:258514/142‑1 (MQ=255)
                                          attgatCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACg                                                                                                                    >  2:258514/1‑142 (MQ=255)
                                          attgatCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGt                                                                                                             <  2:347719/149‑1 (MQ=255)
                                           ttgatCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCAAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGtt                                                                                                            >  1:181328/1‑149 (MQ=255)
                                             gatCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTg                                                                                                           <  8:186081/148‑1 (MQ=255)
                                              atCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTcca                                                                                                                                              >  3:5318/1‑112 (MQ=255)
                                              atCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTcca                                                                                                                                              <  4:5318/112‑1 (MQ=255)
                                                 gAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAg                                                                                                      <  6:2351/149‑1 (MQ=255)
                                                   gCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCa                                                                                                                      <  3:33506/131‑1 (MQ=255)
                                                   gCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCa                                                                                                                      >  4:33506/1‑131 (MQ=255)
                                                     ccTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCAcc                                                                                                  >  2:367059/1‑149 (MQ=255)
                                                      cTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAg                                                                                                                                                     <  3:8522/97‑1 (MQ=255)
                                                      cTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAg                                                                                                                                                     >  4:8522/1‑97 (MQ=255)
                                                      cTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCAccc                                                                                                 >  8:207984/1‑149 (MQ=255)
                                                       ttGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCcg                                                                                                >  4:167009/1‑149 (MQ=255)
                                                       ttGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCcg                                                                                                >  1:37319/1‑149 (MQ=255)
                                                                 tttCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTg                                                                                      <  4:351379/149‑1 (MQ=255)
                                                                 tttCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTg                                                                                      <  4:162384/149‑1 (MQ=255)
                                                                   tCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTgg                                                                                     <  6:354717/148‑1 (MQ=255)
                                                                       cGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTc                                                                                                                                       <  8:188088/94‑1 (MQ=255)
                                                                       cGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTc                                                                                                                                       >  7:188088/1‑94 (MQ=255)
                                                                           gTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAGGCGCTCCAGCCAGTCAGCGAACCAACt                                                                                                                           <  4:363824/102‑1 (MQ=255)
                                                                           gTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAGGCGCTCCAGCCAGTCAGCGAACCAACt                                                                                                                           >  7:268633/1‑102 (MQ=255)
                                                                           gTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAGGCGCTCCAGCCAGTCAGCGAACCAACt                                                                                                                           >  3:363824/1‑102 (MQ=255)
                                                                           gTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAGGCGCTCCAGCCAGTCAGCGAACCAACt                                                                                                                           <  8:268633/102‑1 (MQ=255)
                                                                              gatcgaCGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGc                                                                           >  5:380929/1‑147 (MQ=255)
                                                                                   aCGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCg                                                                    >  6:219305/1‑149 (MQ=255)
                                                                                       cggcTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCa                                                                <  6:380929/149‑1 (MQ=255)
                                                                                        ggcTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAg                                                               <  8:150468/149‑1 (MQ=255)
                                                                                          cTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGgc                                                             <  2:37319/149‑1 (MQ=255)
                                                                                           tCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCa                                                                >  6:299253/1‑145 (MQ=255)
                                                                                           tCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCa                                                                <  5:299253/145‑1 (MQ=255)
                                                                                                    agcGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTc                                                   <  7:195665/149‑1 (MQ=255)
                                                                                                                 cGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCgg                                       <  7:207984/148‑1 (MQ=255)
                                                                                                                  gATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCggg                                      <  5:219305/148‑1 (MQ=255)
                                                                                                                  gATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCggg                                      <  3:167009/148‑1 (MQ=255)
                                                                                                                       ggCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACAcc                                >  7:153272/1‑149 (MQ=255)
                                                                                                                        gCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACca                               <  1:367059/149‑1 (MQ=255)
                                                                                                                          tGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCCGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACcag                              >  7:15952/1‑148 (MQ=255)
                                                                                                                                  ccTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAg                     >  5:314247/1‑149 (MQ=255)
                                                                                                                                         cATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCa              <  8:15952/149‑1 (MQ=255)
                                                                                                                                          aacgcgGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCAc             <  8:45685/147‑1 (MQ=255)
                                                                                                                                          aTCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCAc             >  3:127035/1‑149 (MQ=255)
                                                                                                                                           tCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCACg            <  8:153272/149‑1 (MQ=255)
                                                                                                                                                gTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGa                                                        <  5:305691/100‑1 (MQ=255)
                                                                                                                                                gTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGa                                                        >  6:305691/1‑100 (MQ=255)
                                                                                                                                                   cAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCACGGTCCGgc     <  6:303758/148‑1 (MQ=255)
                                                                                                                                                     aGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCACGGTCCGGcgcg  >  7:241825/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
GCCTCGACCTGAGCGGCGCGCCGCTGCAGCA‑CGGCGCGCTGATTGATCGAGCCCTTGTCGGTGATTTCACCGGCGTCGATCGACGGCGGCTCGGCCAGCAGCGACAGCCATTCGATACGGCTGGCGTTGCCTTGGGCATCGCGGTTCAAGCGCTCCAGCCAGTCAGCGAACCAACTGCGCACGGTGTCGTTGGCCAGCACCCGCGCATCGCTGGCATCCTCTGCCAGCCCGGCCAGGCGCCGACACTCGGGCAGACGCGGGAACACCAGCAGGCCCAGGCATTCACGGTCCGGCGCG  >  NC_002947/3794622‑3794918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑