Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,812,152 C→G 5.3% P159R (CCG→CGG)  PP_3368 → MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,812,1520CG 5.3% 244.8 / 3.8 76P159R (CCG→CGG) PP_3368MFS transporter
Reads supporting (aligned to +/- strand):  ref base C (37/35);  new base G (2/2);  total (39/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01

ATTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTCC  >  NC_002947/3812006‑3812298
                                                                                                                                                    |                                                                                                                                                  
aTTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCgg                                                                                                                                                  >  4:140380/1‑149 (MQ=255)
  ttCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGtt                                                                                                                                                >  7:135507/1‑149 (MQ=255)
   tCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTAGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGtt                                                                                                                                                <  6:362799/148‑1 (MQ=255)
     gTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGGTCGGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGtt                                                                                                                                                <  1:106056/148‑1 (MQ=255)
      tttCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCa                                                                                                                                            <  8:117834/149‑1 (MQ=255)
       ttCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGCCCTGGGCCATGTTGCTCGGCAACACGGTTGCCAc                                                                                                                                           >  8:146383/1‑149 (MQ=255)
        tcatcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGTCCTCGAGCATGGTCGCCAAGTATCAGCGCGAGG‑‑GGGCCGTTGCCCGGGTGATTATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCAc                                                                                                                                           <  2:365/148‑1 (MQ=255)
        tcatcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGGGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCGGGTTGccga                                                                                                                                           <  1:3604/148‑3 (MQ=255)
          atcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCGGGTTGccga                                                                                                                                           >  4:2796/1‑144 (MQ=255)
          atcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCGGGTTGccga                                                                                                                                           <  3:2796/146‑3 (MQ=255)
           tcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTc                                                                                                                                       >  6:234709/1‑149 (MQ=255)
           tcaGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTc                                                                                                                                       >  6:240061/1‑149 (MQ=255)
           tcaGCGGCCTGCCCCACGGCGCCTACTTCGACATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTc                                                                                                                                       >  5:118635/1‑149 (MQ=255)
             aGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCt                                                                                                                                     >  7:135872/1‑149 (MQ=255)
             aGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCt                                                                                                                                     <  3:56464/149‑1 (MQ=255)
              gCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTg                                                                                                                                    >  7:149489/1‑149 (MQ=255)
               cGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTgg                                                                                                                                   <  3:140380/149‑1 (MQ=255)
               cGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTgg                                                                                                                                   <  4:285460/149‑1 (MQ=255)
                   cTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAATGATCATCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCa                                                                                                                               <  7:312869/149‑1 (MQ=255)
                     gCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAg                                                                                                                              >  1:6465/1‑148 (MQ=255)
                        ccACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACt                                                                                                                          >  8:90401/1‑149 (MQ=255)
                           cGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCg                                                                                                                       <  2:6465/149‑1 (MQ=255)
                                 cttcTTCGGCATTGCCGCAGTGGTGGGCTCGTGGATGGTCGCCAAGGATCAGCGGGCGG‑‑GGGCCGTTGCGCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGc                                                                                                                 <  7:164113/146‑1 (MQ=255)
                                 cTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGc                                                                                                                 >  8:346283/1‑149 (MQ=255)
                                    cTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGtt                                                                                                              <  7:90401/149‑1 (MQ=255)
                                     ttCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGcc                                                                                                                                             >  1:134039/1‑117 (MQ=255)
                                     ttCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGcc                                                                                                                                             <  2:134039/117‑1 (MQ=255)
                                     ttCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTc                                                                                                             >  7:125863/1‑149 (MQ=255)
                                     ttCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTc                                                                                                             >  6:110034/1‑149 (MQ=255)
                                          cATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCAt                                                                                                        >  8:111140/1‑149 (MQ=255)
                                           aTTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTcc                                                                                                                                      >  3:349176/1‑118 (MQ=255)
                                           aTTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTcc                                                                                                                                      <  4:349176/118‑1 (MQ=255)
                                            ttGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTc                                                                                                      <  8:135872/149‑1 (MQ=255)
                                            gctccGCAGTGCTGGCCTCGAGCAGGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTc                                                                                                      <  6:199483/146‑1 (MQ=255)
                                               ccGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTg                                                                                                   <  6:118635/149‑1 (MQ=255)
                                                cGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGTCCGTTGCCCGGGTGATGATGGGCCTGACCTTGCCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGTCGTTCGGCATTCgag                                                                                                   >  5:199483/1‑146 (MQ=255)
                                                cGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGc                                                                                                  <  7:111140/149‑1 (MQ=255)
                                                  cAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTg                                                                                                <  8:149489/149‑1 (MQ=255)
                                                              cGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATc                                                                                    >  6:261770/1‑149 (MQ=255)
                                                               gAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGAt                                                                                     <  7:346283/147‑1 (MQ=255)
                                                                   aTGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAATCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCt                                                                               <  7:130613/149‑1 (MQ=255)
                                                                   aTGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCt                                                                               >  2:192149/1‑149 (MQ=255)
                                                                          ccAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCTCTGTGCAc                                                                         >  6:171383/1‑148 (MQ=255)
                                                                             aGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATc                                                                     <  8:135507/149‑1 (MQ=255)
                                                                                        gcgG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTg                                                          >  6:360510/1‑149 (MQ=255)
                                                                                               ggCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGtt                                                     <  5:110034/149‑1 (MQ=255)
                                                                                                 ccGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTc                                                   <  5:261770/149‑1 (MQ=255)
                                                                                                   gTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGt                                                 <  7:146383/149‑1 (MQ=255)
                                                                                                    ttGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTg                                                <  3:297198/149‑1 (MQ=255)
                                                                                                        ccGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGccc                                             >  2:260674/1‑148 (MQ=255)
                                                                                                         cGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGccc                                             >  4:274041/1‑147 (MQ=255)
                                                                                                                 gattGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGcc                                                                  <  6:49371/118‑1 (MQ=255)
                                                                                                                   tgGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCacgac                                 <  5:234709/149‑1 (MQ=255)
                                                                                                                   tgGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCacgac                                 <  5:240061/149‑1 (MQ=255)
                                                                                                                    gggCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCacgac                                 <  8:125863/148‑1 (MQ=255)
                                                                                                                          gACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAacac                          >  5:193937/1‑149 (MQ=255)
                                                                                                                           aCCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACg                         >  7:190490/1‑149 (MQ=255)
                                                                                                                             cTTGGCCATGTTGCTCGGCACCCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACg                         >  5:195874/1‑147 (MQ=255)
                                                                                                                             cTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACg                         >  8:231793/1‑147 (MQ=255)
                                                                                                                              ttGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTACTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAg                      >  1:343305/1‑149 (MQ=255)
                                                                                                                                  ccATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGAc                  >  3:24316/1‑149 (MQ=255)
                                                                                                                                    aTGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGAccc                >  2:195091/1‑149 (MQ=255)
                                                                                                                                     tGTTGCTCGGCAACCCGGTTGCAACATTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTAGTGCTGGTCGGCGCGATCGCACTGTGCACCATCGCCCTGGTATGGCGTTTCGTGCCCCAGCGACACGACGAAACACGCAGCGACAcg               >  8:261644/1‑149 (MQ=255)
                                                                                                                                         gCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCcg               <  6:24458/145‑1 (MQ=255)
                                                                                                                                         gCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCcg               >  5:24458/1‑145 (MQ=255)
                                                                                                                                          ggtggCAACCGGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCgcgc            >  8:200122/4‑147 (MQ=255)
                                                                                                                                           tCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTc                                                   <  6:5654/107‑1 (MQ=255)
                                                                                                                                           tCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTc                                                   >  5:5654/1‑107 (MQ=255)
                                                                                                                                           tCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAg         >  6:134409/1‑149 (MQ=255)
                                                                                                                                             ggCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGc                                               >  6:170005/1‑109 (MQ=255)
                                                                                                                                             ggCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAg         <  6:193937/147‑1 (MQ=255)
                                                                                                                                             ggCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAg         >  5:134752/1‑147 (MQ=255)
                                                                                                                                               cAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGTCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGc     <  7:261644/149‑1 (MQ=255)
                                                                                                                                                 aCCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTc   <  1:260674/149‑1 (MQ=255)
                                                                                                                                                 aCCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTc   <  8:190490/149‑1 (MQ=255)
                                                                                                                                                 aCCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTc   >  1:249801/1‑149 (MQ=255)
                                                                                                                                                  cccGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTcc  <  5:134409/149‑1 (MQ=255)
                                                                                                                                                  cccGGTTGCCACCTTCCTGGGCCAGTACTTCGACTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGt                                                             <  2:217888/90‑1 (MQ=255)
                                                                                                                                                  cccGGTTGCCACCTTCCTGGGCCAGTACTTCGACTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGt                                                             >  1:217888/1‑90 (MQ=255)
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ATTTCGTTTCATCAGCGGCCTGCCCCACGGCGCCTACTTCGGCATTGCCGCAGTGGTGGCCTCGAGCATGGTCGCCAAGGATCAGCGCGCGG‑‑GGGCCGTTGCCCGGGTGATGATGGGCCTGACCTTGGCCATGTTGCTCGGCAACCCGGTTGCCACCTTCCTGGGCCAGTACTTCGGCTGGCGTTCGGCATTCGTGCTGGTCGGCGCGATCGCCCTGTGCACCATCGCCCTGGTCTGGCGTTTCGTGCCCCAGCGCCACGACGAAACACGCAGCGACCCGCGCAAGGAGCTCC  >  NC_002947/3812006‑3812298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑