Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,893,486	T→C	6.0%	F284L (TTC→CTC)	rarD →	chloramphenicol‑sensitive protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,893,486	0	T	C	6.0%	197.8 / 2.5	67	F284L (TTC→CTC)	rarD	chloramphenicol‑sensitive protein
Reads supporting (aligned to +/- strand):  ref base T (36/27);  new base C (2/2);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

CCATTCAGCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCAAGCTCATCAC  >  NC_002947/3893339‑3893633                                                                                                                                                     |                                                                                                                                                     ccATTCAGCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGCTGTGGCGTTGCTGCTGGGCGAAACAATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCt                                                                                                                                                      >  1:283398/1‑149 (MQ=255)   aTTCAGCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTc                                                                                                                                                    <  5:91234/148‑1 (MQ=255)   aTTCAGCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTc                                                                                                                                                    >  6:91234/1‑148 (MQ=255)       aGCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGc                                                                                                                                                <  7:125867/148‑1 (MQ=255)        gCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCAc                                                                                                                                              >  1:9877/1‑149 (MQ=255)        gCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAAGCCCTCAAGCAc                                                                                                                                              <  1:371793/149‑1 (MQ=255)         ccTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCAcc                                                                                                                                             >  7:193678/1‑149 (MQ=255)         ccTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAAGCCCTCAAGCAcc                                                                                                                                             <  4:213530/149‑1 (MQ=255)          cTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCt                                                                                                                                            <  2:283398/149‑1 (MQ=255)           tGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTg                                                                                                                                           >  1:236127/1‑149 (MQ=255)             ttCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGc                                                                                                                                          >  5:163280/1‑148 (MQ=255)                 gCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGt                                                                                                                                     >  7:206513/1‑149 (MQ=255)                 gCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGt                                                                                                                                     <  7:253307/149‑1 (MQ=255)                   cTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCa                                                                                                                                                   >  2:271638/1‑133 (MQ=255)                   cTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCa                                                                                                                                                   <  1:271638/133‑1 (MQ=255)                    tGCTCAGCTACGTCGAACCCGTGCTTCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACATACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTAAGTccg                                                                                                                                  >  2:260219/1‑147 (MQ=255)                      cTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCg                                                                                                                                >  8:28780/1‑149 (MQ=255)                                tCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑g                                                                                                                     <  2:9877/149‑1 (MQ=255)                                tCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑g                                                                                                                     <  1:348786/149‑1 (MQ=255)                                              ctgGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGCTTACACCGAAcg                                                                                                       <  2:236127/149‑2 (MQ=255)                                                  tAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCGGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGc                                                                                                   <  4:42858/149‑1 (MQ=255)                                                  tAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGc                                                                                                   <  7:28780/149‑1 (MQ=255)                                                  tAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGc                                                                                                   <  8:193678/149‑1 (MQ=255)                                                         tggcgtTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTAccc                                                                                             >  7:32995/1‑149 (MQ=255)                                                             gtTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCAcc                                                                                                                                             >  7:178399/1‑98 (MQ=255)                                                             gtTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCAcc                                                                                                                                             <  8:178399/98‑1 (MQ=255)                                                             gtTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCg                                                                                         >  4:345507/1‑149 (MQ=255)                                                             gtTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCg                                                                                         >  2:336812/1‑149 (MQ=255)                                                               tgctgcTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGct                                                                                       >  6:252798/1‑149 (MQ=255)                                                                     tgGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCaa                                                                                 >  2:329074/1‑149 (MQ=255)                                                                       ggCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAAc                                                                                <  8:32995/148‑1 (MQ=255)                                                                         cGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGc                                                                             >  1:173480/1‑149 (MQ=255)                                                                          gAAACCATCGGCCCGGATCAGTGGCAGACCTACCTGCCAAACTGGGCCGCAGTACTGGAGCTGGTGATTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGcc                                                                            >  8:239591/1‑149 (MQ=255)                                                                              ccATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCa                                                                        >  8:288801/1‑149 (MQ=255)                                                                                aTCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACg                                                                      >  7:15340/1‑149 (MQ=255)                                                                                    gCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCa                                                                  <  6:163280/149‑1 (MQ=255)                                                                                    gCCCGGATCAGAGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCa                                                                  <  8:341652/149‑1 (MQ=255)                                                                                     cccGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCAcc                                                                                                                                             >  5:357688/1‑74 (MQ=255)                                                                                     cccGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCAcc                                                                                                                                             <  6:357688/74‑1 (MQ=255)                                                                                     cccGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTgg                                                                                                    >  6:212446/1‑114 (MQ=255)                                                                                     cccGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTgg                                                                                                    <  5:212446/114‑1 (MQ=255)                                                                                     cccGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAg                                                                 >  7:229813/1‑149 (MQ=255)                                                                                         gATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATc                                                             <  2:173480/149‑1 (MQ=255)                                                                                           tCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCa                                                           <  5:252798/149‑1 (MQ=255)                                                                                                     acctacctGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTcc                                                   <  8:15340/147‑1 (MQ=255)                                                                                                       ctacctGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGc                                               >  7:351579/1‑149 (MQ=255)                                                                                                          cctGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCCTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAg                                            >  4:90711/1‑149 (MQ=255)                                                                                                            tGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGcc                                          >  4:103469/1‑149 (MQ=255)                                                                                                             gCCAATCTGGGCCGCAGTACTGGTACTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTTTAAGCCGAACCTGGCGTACCCGTCGATATTCAACCGCCTCCACGGTCAGAAACAAGCCATACCAGGCAAGCCg                                         >  7:235557/1‑149 (MQ=255)                                                                                                              ccAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCg                                         <  1:336812/148‑1 (MQ=255)                                                                                                               cAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGt                                       >  8:319242/1‑149 (MQ=255)                                                                                                                    tGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCAcc                                   >  6:104756/1‑148 (MQ=255)                                                                                                                     gggCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGa                                 >  2:122884/1‑149 (MQ=255)                                                                                                                           cAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCg                           >  4:138780/1‑149 (MQ=255)                                                                                                                                ctggtgctggtgCTTGAGGGCTTCAAGCACCTGCTACGTGAGCGTCGCCGTTC‑GGTGTTAGCCGTACCTGGCGTACGCGTCGCTGTTCAACCTCCTCCACGGTGAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAg                       <  7:239591/148‑1 (MQ=255)                                                                                                                                       tggtgctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCAACGACCATCGGCAGGCGGTCCa               >  7:58326/1‑149 (MQ=255)                                                                                                                                       aggtgctTGAAGCCCTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTc                 >  7:102770/2‑147 (MQ=255)                                                                                                                                       aggtgctTGAAGCCCTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCACCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTc                 >  6:51066/2‑147 (MQ=255)                                                                                                                                         gtgctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGc             <  8:206513/149‑1 (MQ=255)                                                                                                                                          tgctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCa            <  8:229813/149‑1 (MQ=255)                                                                                                                                           gctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTCGGGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCAACCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGc             >  1:258558/1‑148 (MQ=255)                                                                                                                                           gctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTc                 <  3:24926/143‑1 (MQ=255)                                                                                                                                           gctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTc                 >  4:24926/1‑143 (MQ=255)                                                                                                                                            ctTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCAAg          >  5:370167/1‑149 (MQ=255)                                                                                                                                             ttGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCAAg          >  4:357285/1‑148 (MQ=255)                                                                                                                                                  ggCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAg                                                 <  3:130896/104‑1 (MQ=255)                                                                                                                                                  ggCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAg                                                 >  4:130896/1‑104 (MQ=255)                                                                                                                                                  ggCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCAAGCtcatc    <  3:103469/149‑1 (MQ=255)                                                                                                                                                     ttCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCAAGCTCATCAc  >  4:264143/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                     CCATTCAGCCTGTTCGGCCTGCTCAGCTACGTCGAACCCGTGCTGCTGGTAGGC‑GTGGCGTTGCTGCTGGGCGAAACCATCGGCCCGGATCAGTGGCTGACCTACCTGCCAATCTGGGCCGCAGTACTGGTGCTGGTGCTTGAGGGCTTCAAGCACCTGCTACGTCAGCGTCGCCGTTC‑GGTGTAAGCCGAACCTGGCGTACCCGTCGCTCTTCAACCGCCTCCACGGTCAGCATCCAGCCATTCCAGGCTAGCCGGTCACCGACCATCGGCAGGCGGTCCAGCAAGCTCATCAC  >  NC_002947/3893339‑3893633

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑