| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 3,929,797 | G→T | 25.4% | intergenic (+20/+18) | PP_3464 → / ← PP_5603 | hypothetical protein/FAD dependent oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 3,929,797 | 0 | G | T | 25.4% | 52.4 / 16.8 | 36 | intergenic (+20/+18) | PP_3464/PP_5603 | hypothetical protein/FAD dependent oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base G (16/9); new base T (4/5); total (20/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01 | |||||||||||
CCACCTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCA > NC_002947/3929651‑3929939 | ccaccTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGaa < 5:298765/149‑1 (MQ=255) ccgCCCGGGGGTTGGTTTCTACAAGCCGGCGCGCAGGGCGG‑CCAATCGTGGTCCGCAGCTGGGTGGGGGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTCATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCa < 4:72516/147‑1 (MQ=255) ggttTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccagga > 6:302139/1‑132 (MQ=255) ggttTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccagga < 5:302139/136‑5 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGGCCCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCATGGGGCTGCTTCGCAGccccag > 1:191906/1‑131 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccag < 2:191906/132‑3 (MQ=255) tttCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGACGCAGCCCCGGCAATCTGAAACCAAccc > 5:198947/1‑149 (MQ=255) cAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTc > 3:207134/1‑149 (MQ=255) aaGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGccccag < 1:235504/125‑3 (MQ=255) aaGCCGGAGCGAAGTGCGG‑CCAATAGTGGTACGCAGCTGCGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACAACCTCGGGCTCCTTCGCAGccccag > 2:235504/1‑123 (MQ=255) ggAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCATGCTACCCCAg > 7:202941/1‑149 (MQ=255) ccgggCGG‑CCAATCGGGGTCCCCCGGGGGGTGGGCGGGATACCGGGACTAACCTCAAATTCTTTGCGGGCACTTTTTTCTTATCCCACATCGTGGGGGGGCCACGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCAccg < 2:52592/145‑1 (MQ=255) taaTAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGct < 7:287956/148‑1 (MQ=255) aTAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGct > 8:287956/1‑147 (MQ=255) gTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTCGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCTCCCCCAGCCACCGCAGCACGCTCCAcagct > 6:374250/1‑149 (MQ=255) cGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGcccca > 6:290473/1‑78 (MQ=39) cGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCTTCGCAGcccca < 5:290473/79‑2 (MQ=39) ggaacccGCGACTTACCTCCAATTGTGTTGGGGCACCTTTTTCTTACCCGACTTCCGGGGGCGCCGACGAAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGa < 6:198947/144‑1 (MQ=255) cGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCGGCCCCCGCCCCCGCCGCCCGCGGCACTGGTGTTGACCCGCCAGGCCCGCCaaaaa > 3:148089/1‑146 (MQ=255) ttACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCt > 8:113627/1‑148 (MQ=255) gcTGCATTTTATTGTCGGGACATTTTTATGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCaag < 5:374250/148‑2 (MQ=255) ccTGCAATTTATTGTGGGTACTTTTTTTTTATCCGACATCCTGGGGGTGCTAATAAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCaa < 8:202941/148‑1 (MQ=255) ccTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCaa > 3:156936/1‑148 (MQ=255) cAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTGCCCCAGCCACCGCCGCACGCTCCTCTGCTGTAGACCCGCAAGCCCCGACAATACCCACTCAACATgc > 7:155228/1‑149 (MQ=255) aaTTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGgcc < 7:113627/149‑1 (MQ=255) aaTTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCCCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACCATACCGCCTCAACAGgcc > 7:270940/1‑149 (MQ=255) attgTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCa > 1:243145/1‑149 (MQ=255) gACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCAGCTCCCCCCGCCACCGCCCCACGCACCGCGGCGGTTAACCCCCCAGCCCCGACAAAACACCCGCAACAGGCCGCCCCCAGCGTaa > 6:144463/1‑148 (MQ=255) cacttttttGTTATCCTACATCCTGGGGCTGCCAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTAt < 4:156936/145‑1 (MQ=255) ttCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCg > 1:63600/1‑148 (MQ=255) aTCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCGCCCCCCGCCACCGCCGCACGCGCCTCTGCGGTTGACCCCCCAGCCCCGCCAATACCGACTGAACAGGCCGCCCACTGCGGATTTGTCGcgccca > 4:209948/1‑147 (MQ=255) cGACATCCTGGGGCTGCGAAGCAGCACCGGCAATCTCAAAACAACCCCAGCTCCCCCAGCCCCCGCCCCACGCTCCGCAGCAGTTAACCCCGAAGCCCCGACCATACCGCCGCAACAGGCCGCCCACTGCGTATTTGTAGCACCACCgg > 6:109240/1‑149 (MQ=255) cttcggggggCTGGGAACCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGgcgc < 8:155228/143‑1 (MQ=255) gggCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGtt < 2:243145/147‑1 (MQ=255) ggCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCCGCCACCGCCGCACGCTCCTCTGCTGTTGACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGGATTTGTCGCCCCACCGGGGCCAGttacc > 6:78461/1‑146 (MQ=255) ggCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCa < 2:63600/149‑1 (MQ=255) | CCACCTGCTGATCAGCCCGGTGGTTGGTTTCTACAAGCCGGAGCGCAGTGCGG‑CCAATAGTGGTACGCAGCTGGGTGGGCGGGATACCGGGACTTACCTGCAATTGATTGTCGGGACCTTTTTCTGATCCGACATCCTGGGGCTGCGAAGCAGCCCCGGCAATCTCAAACCAACCCCTGCTCCCCCAGCCACCGCCGCACGCTCCTCTGCTGTTCACCCGCAAGCCCCGACAATACCGCCTCAACAGGCCGCCCACTGCGTATTTGTCGCACCACCGGCGCCAGTTCCA > NC_002947/3929651‑3929939 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |