Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,944,713 G→C 9.8% D82E (GAC→GAG)  PP_3480 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,944,7130GC 9.8% 143.8 / 7.2 51D82E (GAC→GAG) PP_3480hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (23/23);  new base C (2/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.44e-01

GTAGCGGGCCAGCTGGGTCTTGGGGTCGATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGCCGGCACACCCAACGCCAAT  >  NC_002947/3944569‑3944859
                                                                                                                                                |                                                                                                                                                   
gTAGCGGGCCAGCTGGGTCTTGGGGTCGATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTc                                                                                                                                                   <  8:337847/147‑1 (MQ=255)
       gCCAGCTGGGTCTTGGGGTCGATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCctg                                                                                                                                          <  7:171422/149‑1 (MQ=255)
       gCCAGCTGGGTCTTGGGGTCGATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCAGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCctg                                                                                                                                          >  5:51856/1‑149 (MQ=255)
        ccAGCTGGGTCTTGGGGTCGATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCCCTCGACAGCctgc                                                                                                                                         <  5:187327/149‑1 (MQ=255)
                           gATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCaa                                                                                                                     >  1:370383/1‑149 (MQ=255)
                            aTACCCAACGCGTATTCACCGCGGGCCAGGCAGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAAt                                                                                                                    >  1:228064/1‑149 (MQ=255)
                             tACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATc                                                                                                                   <  1:79001/149‑1 (MQ=255)
                             tACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCACAATc                                                                                                                   <  3:187314/149‑1 (MQ=255)
                                ccAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGTCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTg                                                                                                                             >  6:345381/1‑136 (MQ=255)
                                ccAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGGGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTg                                                                                                                             <  5:345381/136‑1 (MQ=255)
                                ccAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAg                                                                                                                                                <  6:318786/118‑1 (MQ=255)
                                ccAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAg                                                                                                                                                >  5:318786/1‑118 (MQ=255)
                                 cAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCCCTCGACAGCctgcgcca                                                                                                                                     >  3:170064/1‑124 (MQ=255)
                                 cAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCCCTCGACAGCctgcgcca                                                                                                                                     <  4:170064/128‑5 (MQ=255)
                                  aaCGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAg                                                                                                              <  3:333368/149‑1 (MQ=255)
                                   aCGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGc                                                                                                             >  7:345744/1‑149 (MQ=255)
                                     gcgTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑Tc                                                                                                                                   >  7:346046/1‑125 (MQ=255)
                                     gcgTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑Tc                                                                                                                                   <  8:346046/125‑1 (MQ=255)
                                          ttCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCCCTCGACAGCctgcgcca                                                                                                                                     <  1:148165/119‑5 (MQ=255)
                                          ttCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCCCTCGACAGCctgcgcca                                                                                                                                     >  2:148165/1‑115 (MQ=255)
                                           tCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCg                                                                                                     >  6:325678/1‑149 (MQ=255)
                                            cACCGCGGGCCAGGCTGATACGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TAGGGCTGGACCGCAATCTTCAGCTCACCCCGc                                                                                                    >  4:95730/1‑149 (MQ=255)
                                                      cAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCTACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCACGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGCACGGAATCTTCAGCTCACCCCGCTAGACACGcc                                                                                          >  6:287220/1‑149 (MQ=255)
                                                       aGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGccc                                                                                         >  5:133728/1‑149 (MQ=255)
                                                           tGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCATGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTAcc                                                                                     >  3:72742/1‑149 (MQ=255)
                                                            gATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAgg                                                                                                                                               <  2:67317/91‑1 (MQ=255)
                                                            gATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAgg                                                                                                                                               >  1:67317/1‑91 (MQ=255)
                                                             aTGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTg                                                                                   >  1:351757/1‑149 (MQ=255)
                                                               gcgcTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGtt                                                                                 >  1:54063/1‑149 (MQ=255)
                                                                     ggCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCAGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTg                                                                            <  6:51856/148‑1 (MQ=255)
                                                                      gcgcTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGc                                                                          >  6:145526/1‑149 (MQ=255)
                                                                              cAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCtt                                                                  >  7:309270/1‑149 (MQ=255)
                                                                               aGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTc                                                                 <  1:13556/149‑1 (MQ=255)
                                                                                   ggTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTg                                                             <  2:351757/149‑1 (MQ=255)
                                                                                     tACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGac                                                           <  2:370383/149‑1 (MQ=255)
                                                                                            gCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGt                                                    >  2:59129/1‑149 (MQ=255)
                                                                                                gATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTcgc                                                >  4:152881/1‑149 (MQ=255)
                                                                                                 aTCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTAGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTcgcg                                               <  2:228064/149‑1 (MQ=255)
                                                                                                  tCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGt                                              <  8:345744/149‑1 (MQ=255)
                                                                                                     aCTCGCTCGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTACGCTGGCCTCCGCTTCGGTGACACGAAGATCGCGTACg                                           >  4:322320/1‑149 (MQ=255)
                                                                                                          cTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTTTCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTg                                      <  3:95730/149‑1 (MQ=255)
                                                                                                           tGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGCGTCGGGCTGGACCGCAATCTTCAGCTCCCCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTg                                      >  1:283562/1‑149 (MQ=255)
                                                                                                           tGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGc                                     >  7:351676/1‑149 (MQ=255)
                                                                                                             gCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCa                                   <  5:325678/149‑1 (MQ=255)
                                                                                                                  gggCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTg                                <  1:59129/147‑1 (MQ=255)
                                                                                                                  gggCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGc                               <  5:145526/148‑1 (MQ=255)
                                                                                                                            ggCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGcc                    <  8:309270/149‑1 (MQ=255)
                                                                                                                             gCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGCCg                   <  2:54063/149‑1 (MQ=255)
                                                                                                                                 ttGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGCCGGca                >  2:241806/1‑148 (MQ=255)
                                                                                                                                              cTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGCCGGCACACCCAACGCCAAt  <  3:152881/149‑1 (MQ=255)
                                                                                                                                              cTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGCCGGCACACCCAACGCCAAt  <  1:241806/149‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GTAGCGGGCCAGCTGGGTCTTGGGGTCGATACCCAACGCGTATTCACCGCGGGCCAGGCTGATGCGCTCGGCGCTGGCCAGACGGTACAGACGCTCGATCAACTCGCTGGCCTGGGGCTGACCGGGCAGTTGCTGGCGCAGGCTGTCGAGGGCCTGCTGC‑TCGGGCTGGACCGCAATCTTCAGCTCACCCCGCTTGACACGCCCTACCTGTTCGCTGGCCTGCGCTTCGGTGACACGCAGTTCGCGTACGCTTTGCCATTGCGGCAGCACGCCGGCACACCCAACGCCAAT  >  NC_002947/3944569‑3944859

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑