| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,201,454 | C→G | 7.0% | V2108L (GTC→CTC) | PP_3691 ← | DNA helicase‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,201,454 | 0 | C | G | 7.0% | 176.8 / 3.6 | 57 | V2108L (GTC→CTC) | PP_3691 | DNA helicase‑related protein |
| Reads supporting (aligned to +/- strand): ref base C (27/26); new base G (2/2); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
AAATTTCTCCGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCTCGCCTCAAAAAA > NC_002947/4201310‑4201601 | aaaTTTCTCCGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGa > 3:148122/1‑149 (MQ=255)aaaTTTCTCCGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGa > 8:344378/1‑149 (MQ=255)aaaTTTCTCCGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGa > 2:104882/1‑149 (MQ=255) cGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGTCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAAt < 6:245970/148‑1 (MQ=255) cGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGaa < 4:327954/147‑1 (MQ=255) cGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATg > 3:121625/1‑149 (MQ=255) cGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGAAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGAGTCGATCaaata < 4:61085/147‑5 (MQ=255) gCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGt < 5:361179/149‑1 (MQ=255) gggCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGa < 4:121625/149‑1 (MQ=255) gCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCGTTGTCCGCGCTATTTAATCGACTCGATCACGAATGCGTGAGCCCGtt < 1:81081/148‑1 (MQ=255) atcatcACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTCAGCCCGTTCTGa > 5:49358/1‑149 (MQ=255) catcaCCCGGCCTGCGGAAGGCAACATTAGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCAGGCCTGCTCTTCTTCATTGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCACGTTCTGAcc > 2:229135/1‑149 (MQ=255) atccCCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGTAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGAcc < 7:254522/148‑1 (MQ=255) aCCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGc > 4:212684/1‑149 (MQ=255) aCCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGAGTGAGCCCGTTCTGACCCAGc > 7:5172/1‑149 (MQ=255) ccGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCa > 1:8926/1‑149 (MQ=255) ccGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCa < 1:38832/149‑1 (MQ=255) cGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCAt < 4:148122/149‑1 (MQ=255) cGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTCAGCCCGTTCTGACCCAGCCAt < 6:49358/149‑1 (MQ=255) gCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATgc < 3:155224/149‑1 (MQ=255) cTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATgcgc < 2:51508/149‑1 (MQ=255) ctactttCGAATATGAAACGAGGTGAGCAGGCAAGAAGAAGTTTCCGGACTGCTCTTCTTCTTGTAATCGTACTCG‑TGTCCGCGCTATTTGATCGACTCGAGCACGAATGCGTGAGCCCGTTCTTACCCACCCATGCGCTCGCTCGAt < 5:297226/143‑1 (MQ=255) aaCATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑c > 4:193102/1‑149 (MQ=255) gAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGc < 2:8926/149‑1 (MQ=255) aTCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGc > 5:359049/1‑147 (MQ=255) tGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAgcg > 8:354523/1‑149 (MQ=255) aGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATc < 3:193102/149‑1 (MQ=255) gCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGc > 4:47438/1‑149 (MQ=255) cAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGccc < 1:104882/149‑1 (MQ=255) aagaagGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCctc > 1:341476/1‑149 (MQ=255) cTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGGGTACAGGCCCCTCTTCTGCAACTACAt < 6:359049/149‑1 (MQ=255) ctctTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATgg > 3:118405/1‑148 (MQ=255) ttcATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAatca > 2:363959/1‑149 (MQ=255) tcATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATCGGCAatcat > 2:348424/1‑149 (MQ=255) cATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAatcatc > 4:356611/1‑149 (MQ=255) aTGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCt > 8:292922/1‑149 (MQ=255) aTGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGAGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CACCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAatcatc < 8:5172/148‑1 (MQ=255) tGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTg > 2:203845/1‑149 (MQ=255) tcggaatcg‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTcc > 8:328427/1‑148 (MQ=255) ggaatcg‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCaaa > 1:149907/1‑149 (MQ=255) g‑tgtCCTCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGtt < 1:365283/149‑1 (MQ=255) g‑tgtCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGtt < 1:363959/149‑1 (MQ=255) tCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGt < 2:149907/148‑1 (MQ=255) ccGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGta > 1:118814/1‑148 (MQ=255) cgcgCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGtat < 1:229135/148‑1 (MQ=255) cgcgCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGtat < 7:292922/148‑1 (MQ=255) gcgcTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATATTGCCGTGCAAGCGCCGTATAGAGTACAGGCCCCTCTTATGCAACAACATGGGCAATAATCAGCTCCAAAGTGGTTGtt > 1:115141/1‑146 (MQ=255) gcTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGta > 6:373152/1‑144 (MQ=255) gcTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGta < 5:373152/144‑1 (MQ=255) gcTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCt < 3:356611/149‑1 (MQ=255) cattcgtGATCGAGTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGtt > 7:47405/7‑141 (MQ=255) cattcgtGATCGAGTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGtt < 8:47405/135‑1 (MQ=255) ttcgtGATCGAGTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCTCg > 8:316200/5‑149 (MQ=255) tGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCTCGCCt < 7:354523/148‑1 (MQ=255) tCGACTCGATCACAAATGCGTGAGCCCAATCTGCCCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGACTACAGGCCCCTCTTCTGCAACTACATGGGCAATCAACTGCTCCAAAGTGGTTGTATAGCTCGCCTCaa > 8:55500/1‑148 (MQ=255) gACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCTCGCCTCaaaaa < 2:118814/149‑1 (MQ=255) aCTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCTCGCCTCaaaaaa > 4:124403/1‑149 (MQ=255) | AAATTTCTCCGAGCGGGCGAATTGCATCATCACCCGGCCTGCGGAAGGCAACATTCGAATCTGAACCGAGGTGATCAGGCCAGAAGAAGGTTCCGGCCTGCTCTTCTTCATGGAATCGGAATCG‑TGTCCGCGCTATTTGATCGACTCGATCACGAATGCGTGAGCCCGTTCTGACCCAGCCATGCGCGCGCGCGATA‑CGCCGTGCAAGCGCCGTATCGAGTACAGGCCCCTCTTCTGCAACTACATGGGCAATCATCTGCTCCAAAGTGGTTGTATAGCTCGCCTCAAAAAA > NC_002947/4201310‑4201601 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |