Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,267,946 A→T 12.5% intergenic (‑89/+14) galP‑IV ← / ← PP_3740 porin‑like protein/MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,267,9460AT12.5% 123.0 / 8.4 48intergenic (‑89/+14)galP‑IV/PP_3740porin‑like protein/MFS transporter
Reads supporting (aligned to +/- strand):  ref base A (22/20);  new base T (4/2);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01

CCAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTGCCAGCCA  >  NC_002947/4267806‑4268089
                                                                                                                                            |                                                                                                                                               
ccAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTGGCCggg                                                                                                                                          >  1:254130/1‑148 (MQ=255)
ccAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTGGCCggg                                                                                                                                          >  1:368376/1‑148 (MQ=255)
 cAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTGGCCGGGta                                                                                                                                        <  2:254130/149‑2 (MQ=255)
 cAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCTGGCCGGGta                                                                                                                                        <  2:368376/149‑2 (MQ=255)
     gAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGATGCATTCAGATACCCGGCCAGGCCGGGTTaca                                                                                                                                     >  5:344727/1‑148 (MQ=255)
          aGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGCCCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGt                                                                                                                               >  3:64995/1‑149 (MQ=255)
          aGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGt                                                                                                                               >  4:98610/1‑149 (MQ=255)
            ccAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTgg                                                                                                                             >  3:212326/1‑149 (MQ=255)
                gACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCtga                                                                                                                         <  6:344727/149‑3 (MQ=255)
                  ccGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGt                                                                                                                       <  4:366066/149‑1 (MQ=255)
                      aCGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTTTTCTCCACGTGCCATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCAt                                                                                                                   <  7:223586/149‑1 (MQ=255)
                                gTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCaggc                                                                                                           >  5:65138/1‑147 (MQ=255)
                                               atcatTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACTCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCtt                                                                                          >  1:105745/1‑149 (MQ=255)
                                               atcatTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCAAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGAAAGGCAGACGCAGCCtt                                                                                          >  8:231489/1‑149 (MQ=255)
                                                  attattattGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGc                                                                                       >  8:184255/1‑149 (MQ=255)
                                                     attattGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCg                                                                                     <  4:212326/148‑1 (MQ=255)
                                                      ttattGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCgg                                                                                                                                           >  6:308555/1‑93 (MQ=255)
                                                      ttattGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCgg                                                                                                                                           <  5:308555/93‑1 (MQ=255)
                                                         ttGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTgg                                                                                 <  3:98610/148‑1 (MQ=255)
                                                         ttGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTgg                                                                                 <  8:172934/148‑1 (MQ=255)
                                                           gTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGgc                                                                               <  6:65138/148‑1 (MQ=255)
                                                            ttCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGTCAAGGCAGACGCAGGCTTGGCCGTTg                                                                                  <  7:190637/144‑1 (MQ=255)
                                                            ttCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGTCAAGGCAGACGCAGGCTTGGCCGTTg                                                                                  >  8:190637/1‑144 (MQ=255)
                                                            ttCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGgcg                                                                              >  4:270660/1‑148 (MQ=255)
                                                               tcCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCaa                                                                           >  3:237884/1‑148 (MQ=255)
                                                                 cACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGt                                                                                                                               <  7:316049/94‑1 (MQ=255)
                                                                 cACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGt                                                                                                                               >  8:316049/1‑94 (MQ=255)
                                                                     ttCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcagc                                                                    <  1:23094/149‑1 (MQ=255)
                                                                       cAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcagca                                                                   <  3:270660/148‑1 (MQ=255)
                                                                                          ccccAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCAc                                                >  6:49248/1‑148 (MQ=255)
                                                                                                 ccAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCAGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGc                                                      >  3:129605/1‑135 (MQ=255)
                                                                                                 ccAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCAGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGc                                                      <  4:129605/135‑1 (MQ=255)
                                                                                                   aGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTg                                      >  1:315798/1‑149 (MQ=255)
                                                                                                   aGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTg                                      <  4:237884/149‑1 (MQ=255)
                                                                                                             ggAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGc                            <  2:105745/149‑1 (MQ=255)
                                                                                                               aGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCa                          <  4:64995/149‑1 (MQ=255)
                                                                                                                 cAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcag                                                                     <  1:349999/104‑1 (MQ=255)
                                                                                                                 cAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAAcagcag                                                                     >  2:349999/1‑104 (MQ=255)
                                                                                                                           cATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAg               <  2:315798/148‑1 (MQ=255)
                                                                                                                            aTTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGTCGCAACAGCAGCAGTCAACCTGCGGCAAGCACGAACACGCt                                       >  7:11444/1‑123 (MQ=255)
                                                                                                                            aTTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCt                                       <  8:11444/123‑1 (MQ=255)
                                                                                                                            aTTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAggg             >  7:337457/1‑149 (MQ=255)
                                                                                                                              tCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGt           >  3:75698/1‑149 (MQ=255)
                                                                                                                               cAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGtt          >  6:128106/1‑149 (MQ=255)
                                                                                                                               cAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGt           <  8:337457/148‑1 (MQ=255)
                                                                                                                                tgtaaCCCGGCCTGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTg         >  6:86469/5‑149 (MQ=255)
                                                                                                                                  taaCCCGGCCTGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGttct        >  5:325976/3‑146 (MQ=255)
                                                                                                                                       cGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACATGCCATACAGATGCAGGGGTTgccagcca  >  5:194377/1‑149 (MQ=255)
                                                                                                                                            |                                                                                                                                               
CCAGCGAAACAGCCAGGACCGAACGGGCCAGCGTGCAAGTTGTGTCCATCATTATTATTGTTCTCCACGTTCAATAGGCGCAGCAGACCGCCCCAGGCCAGCATGACCCGGAGCAGTAAGTTGCATTCAGATACCCGGCCAGGCCGGGTTACACTCAGTGGCTCAGTGCATCAGCAGGCAAGGCAGACGCAGGCTTGGCCGTTGGGCGCAACAGCAGCAGGCAACCTGCGGCAAGCACGAACACGCTGGCGAACACGCCATACAGATGCAGGGGTTGCCAGCCA  >  NC_002947/4267806‑4268089

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑