| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 4,289,430 | T→G | 6.6% | K1053Q (AAG→CAG) | PP_3761 ← | two‑component system sensor histidine kinase/response regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 4,289,430 | 0 | T | G | 6.6% | 192.4 / 5.0 | 61 | K1053Q (AAG→CAG) | PP_3761 | two‑component system sensor histidine kinase/response regulator |
| Reads supporting (aligned to +/- strand): ref base T (30/27); new base G (2/2); total (32/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
CGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGT > NC_002947/4289284‑4289576 | cgGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTtgt < 1:251631/149‑1 (MQ=255) cTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTc > 5:110844/1‑147 (MQ=255) tCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca < 4:308656/149‑3 (MQ=255) tCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGACTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg < 6:346555/149‑1 (MQ=255) cAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg < 4:23594/148‑1 (MQ=255) cGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAg < 3:306178/149‑1 (MQ=255) gggTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAgg < 8:6636/149‑1 (MQ=255) gTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAggg > 8:220511/1‑148 (MQ=255) cTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCa > 5:342233/1‑145 (MQ=255) cTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCa > 4:293505/1‑145 (MQ=255) tCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGa > 7:333803/1‑148 (MQ=255) aaGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATAtt < 4:140104/149‑1 (MQ=255) aaGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATAtt < 7:75574/149‑1 (MQ=255) aGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTa > 5:66601/1‑149 (MQ=255) gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTCGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGca > 6:84824/1‑149 (MQ=255) aTCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACAtc < 3:293505/149‑1 (MQ=255) atcatcACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccac < 6:110844/149‑1 (MQ=255) atcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccacca < 8:6211/148‑1 (MQ=255) tcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACcagc < 6:66601/149‑1 (MQ=255) cGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCagcagc < 6:41692/149‑1 (MQ=255) gTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAgcagca > 2:258192/1‑149 (MQ=255) tCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCAc > 1:337804/1‑149 (MQ=255) tCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTg > 7:16081/1‑149 (MQ=255) cACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGc > 2:104310/1‑149 (MQ=255) tatCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACAcc < 2:337804/149‑1 (MQ=255) atCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCt > 7:223467/1‑149 (MQ=255) tcgtcgTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg < 8:271020/84‑1 (MQ=255) tcgtcgTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg > 7:271020/1‑84 (MQ=255) tcgtcgTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCtt < 8:333803/149‑1 (MQ=255) tgctCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca > 8:41544/1‑73 (MQ=37) tgctCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca < 7:41544/75‑3 (MQ=37) aGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTCCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAg > 6:372881/1‑81 (MQ=255) aGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTCCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAg < 5:372881/81‑1 (MQ=255) gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt > 5:277660/1‑147 (MQ=255) gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt < 1:258192/147‑1 (MQ=255) aTGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAg > 4:97156/1‑149 (MQ=255) ggCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt < 8:274405/143‑1 (MQ=255) ggCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt > 7:274405/1‑143 (MQ=255) ggCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACATTGTCGCGGCTGCGCGCGGACtttcgca > 8:296831/1‑144 (MQ=255) cTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCAACTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATg > 4:141050/1‑149 (MQ=255) ttCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGc > 6:25009/1‑149 (MQ=255) cgcgCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGc < 6:342233/147‑1 (MQ=255) gcgcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGtt > 4:293923/1‑149 (MQ=255) gcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt < 3:257996/135‑1 (MQ=255) gcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt > 4:257996/1‑135 (MQ=255) ggCCGATTTCTACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGTGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCtt < 4:228511/106‑1 (MQ=255) ggCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg < 5:84824/149‑1 (MQ=255) ggCCGATTTAGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCtt > 3:228511/1‑106 (MQ=255) ccGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGa > 5:185942/1‑149 (MQ=255) cGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGAc < 3:293923/149‑1 (MQ=255) gACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg > 2:223025/1‑139 (MQ=255) gACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg < 1:223025/139‑1 (MQ=255) gACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGc < 3:97156/148‑1 (MQ=255) cAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt > 4:94516/1‑149 (MQ=255) cAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt < 8:16081/149‑1 (MQ=255) cAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATCACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt < 4:344645/149‑1 (MQ=255) aaTGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAtt > 1:46447/1‑149 (MQ=255) cagcgcCCTGGAGCACAAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc > 4:273294/3‑148 (MQ=255) gcgcCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGAc > 8:116777/1‑149 (MQ=255) gcCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCt > 5:94237/1‑149 (MQ=255) ccTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTtg > 1:339112/1‑149 (MQ=255) cTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTtgt < 1:104310/149‑1 (MQ=255) | CGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGT > NC_002947/4289284‑4289576 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |