Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,289,432 T→A 6.5% H1052L (CAC→CTC)  PP_3761 ← two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,289,4320TA 6.5% 188.8 / 3.1 61H1052L (CAC→CTC) PP_3761two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base T (30/27);  new base A (2/2);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

CGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTGC  >  NC_002947/4289284‑4289578
                                                                                                                                                    |                                                                                                                                                  
cgGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTtgt                                                                                                                                                    <  1:251631/149‑1 (MQ=255)
      cTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTc                                                                                                                                                >  5:110844/1‑147 (MQ=255)
              tCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca                                                                                                                                      <  4:308656/149‑3 (MQ=255)
              tCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGACTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg                                                                                                                                      <  6:346555/149‑1 (MQ=255)
               cAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg                                                                                                                                      <  4:23594/148‑1 (MQ=255)
                   cGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAg                                                                                                                                 <  3:306178/149‑1 (MQ=255)
                    gggTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAgg                                                                                                                                <  8:6636/149‑1 (MQ=255)
                      gTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAggg                                                                                                                               >  8:220511/1‑148 (MQ=255)
                           cTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCa                                                                                                                             >  4:293505/1‑145 (MQ=255)
                           cTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCa                                                                                                                             >  5:342233/1‑145 (MQ=255)
                            tCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGa                                                                                                                         >  7:333803/1‑148 (MQ=255)
                               aaGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATAtt                                                                                                                     <  4:140104/149‑1 (MQ=255)
                               aaGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATAtt                                                                                                                     <  7:75574/149‑1 (MQ=255)
                                aGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTa                                                                                                                    >  5:66601/1‑149 (MQ=255)
                                    gTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTCGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGca                                                                                                                >  6:84824/1‑149 (MQ=255)
                                        aTCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACAtc                                                                                                            <  3:293505/149‑1 (MQ=255)
                                                 atcatcACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccac                                                                                                   <  6:110844/149‑1 (MQ=255)
                                                    atcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTccacca                                                                                                 <  8:6211/148‑1 (MQ=255)
                                                     tcaCGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACcagc                                                                                               <  6:66601/149‑1 (MQ=255)
                                                        cGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCagcagc                                                                                            <  6:41692/149‑1 (MQ=255)
                                                         gTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAgcagca                                                                                           >  2:258192/1‑149 (MQ=255)
                                                          tCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCAc                                                                                          >  1:337804/1‑149 (MQ=255)
                                                              tCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTg                                                                                      >  7:16081/1‑149 (MQ=255)
                                                                  cACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGc                                                                                  >  2:104310/1‑149 (MQ=255)
                                                                             tatCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACAcc                                                                       <  2:337804/149‑1 (MQ=255)
                                                                              atCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCt                                                                      >  7:223467/1‑149 (MQ=255)
                                                                               tcgtcgTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg                                                                                                                                      <  8:271020/84‑1 (MQ=255)
                                                                               tcgtcgTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTg                                                                                                                                      >  7:271020/1‑84 (MQ=255)
                                                                               tcgtcgTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCtt                                                                     <  8:333803/149‑1 (MQ=255)
                                                                                        tgctCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca                                                                                                                                      >  8:41544/1‑73 (MQ=37)
                                                                                        tgctCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTGGAGCACAAGGgcgcca                                                                                                                                      <  7:41544/75‑3 (MQ=37)
                                                                                              aGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTCCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAg                                                                                                                          >  6:372881/1‑81 (MQ=255)
                                                                                              aGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTCCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAg                                                                                                                          <  5:372881/81‑1 (MQ=255)
                                                                                                      gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt                                                <  1:258192/147‑1 (MQ=255)
                                                                                                      gATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCtt                                                >  5:277660/1‑147 (MQ=255)
                                                                                                       aTGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAg                                             >  4:97156/1‑149 (MQ=255)
                                                                                                         ggCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt                                                 >  7:274405/1‑143 (MQ=255)
                                                                                                         ggCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt                                                 <  8:274405/143‑1 (MQ=255)
                                                                                                         ggCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACATTGTCGCGGCTGCGCGCGGACtttcgca                                           >  8:296831/1‑144 (MQ=255)
                                                                                                           cTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCAACTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATg                                         >  4:141050/1‑149 (MQ=255)
                                                                                                            ttCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGc                                        >  6:25009/1‑149 (MQ=255)
                                                                                                              cgcgCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGc                                        <  6:342233/147‑1 (MQ=255)
                                                                                                               gcgcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGtt                                     >  4:293923/1‑149 (MQ=255)
                                                                                                                 gcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt                                                 >  4:257996/1‑135 (MQ=255)
                                                                                                                 gcCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCt                                                 <  3:257996/135‑1 (MQ=255)
                                                                                                                          ggCCGATTTCTACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGTGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCtt                                                                     <  4:228511/106‑1 (MQ=255)
                                                                                                                          ggCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg                          <  5:84824/149‑1 (MQ=255)
                                                                                                                          ggCCGATTTAGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCtt                                                                     >  3:228511/1‑106 (MQ=255)
                                                                                                                            ccGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGa                        >  5:185942/1‑149 (MQ=255)
                                                                                                                             cGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGAc                       <  3:293923/149‑1 (MQ=255)
                                                                                                                                    gACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg                          >  2:223025/1‑139 (MQ=255)
                                                                                                                                    gACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTg                          <  1:223025/139‑1 (MQ=255)
                                                                                                                                    gACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGc                 <  3:97156/148‑1 (MQ=255)
                                                                                                                                      cAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt              >  4:94516/1‑149 (MQ=255)
                                                                                                                                      cAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt              <  8:16081/149‑1 (MQ=255)
                                                                                                                                      cAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATCACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAt              <  4:344645/149‑1 (MQ=255)
                                                                                                                                       aaTGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGAtt             >  1:46447/1‑149 (MQ=255)
                                                                                                                                         cagcgcCCTGGAGCACAAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTc            >  4:273294/3‑148 (MQ=255)
                                                                                                                                           gcgcCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGAc         >  8:116777/1‑149 (MQ=255)
                                                                                                                                             gcCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCt       >  5:94237/1‑149 (MQ=255)
                                                                                                                                               ccTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTtg     >  1:339112/1‑149 (MQ=255)
                                                                                                                                                cTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTtgt    <  1:104310/149‑1 (MQ=255)
                                                                                                                                                   gtgCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTGc  <  3:94516/148‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                  
CGGTTGCTGGCGGATCAGGCGGGTGGCCTCGAAGCCGTCCATCTCCGGCATCATCACGTCCATCAGCACCAGGTCGATATCGTCGTGCTGCTCCAGGCGCTCGATGGCTTCGCGCCCGTTGCGGCCGATTTCGACAATGGCGCCCTTGTGCTCCAGGGCGCTGGTCAGGGCAAAGATATTACGCACATCGTCGTCCACCAGCAGCACCTTGCGGCCCTCGAACACCTTGTCGCGGCTGCGCGCGGTCTTGAGCATGCGTTGACGTTCGTTGGACAGCTGCGATTCGACCTTGTGC  >  NC_002947/4289284‑4289578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑