Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,316,025 G→T 6.2% A36A (GCG→GCT)  PP_3788 → non‑ribosomal peptide synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,316,0250GT 6.2% 255.8 / 6.2 80A36A (GCG→GCT) PP_3788non‑ribosomal peptide synthetase
Reads supporting (aligned to +/- strand):  ref base G (39/36);  new base T (3/2);  total (42/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.46e-01

CAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGTTGTC  >  NC_002947/4315877‑4316172
                                                                                                                                                    |                                                                                                                                                    
caGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCg                                                                                                                                                      >  7:210933/1‑149 (MQ=255)
     aaTCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCAtt                                                                                                                                                 >  6:305890/1‑149 (MQ=255)
     aaTCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCAtt                                                                                                                                                 <  1:53113/149‑1 (MQ=255)
      aTCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTc                                                                                                                                                >  5:344542/1‑149 (MQ=255)
          gTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgatg                                                                                                                                            <  4:214890/149‑3 (MQ=255)
                tACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGAc                                                                                                                                       >  5:176253/1‑148 (MQ=255)
                  cGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCa                                                                                                                                     <  7:127656/148‑1 (MQ=255)
                  cGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAg                                                                                                                                    <  4:8198/149‑1 (MQ=255)
                    ccACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgat                                                                                                                                             >  2:341382/1‑137 (MQ=255)
                    ccACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCCGCTGCATTcgat                                                                                                                                             <  1:341382/138‑2 (MQ=255)
                     cacacaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAgcgc                                                                                                                                 >  6:188981/1‑149 (MQ=255)
                     cacacaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAgcgc                                                                                                                                 >  2:218185/1‑149 (MQ=255)
                     cacacaGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAgcgc                                                                                                                                 >  2:47147/1‑149 (MQ=255)
                          aGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAc                                                                                                                            <  5:305890/149‑1 (MQ=255)
                           gCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAcc                                                                                                                           >  6:243061/1‑149 (MQ=255)
                           gCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCATACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAcc                                                                                                                           <  1:47147/149‑1 (MQ=255)
                            cAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGAcc                                                                                                                           >  6:40638/1‑148 (MQ=255)
                               ggAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTa                                                                                                                         >  1:145162/1‑147 (MQ=255)
                               ggAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACa                                                                                                                       <  8:210933/149‑1 (MQ=255)
                                    gcagcaTGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                                  <  6:93972/149‑1 (MQ=255)
                                            aaCCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGa                                                                                                           <  6:304210/148‑1 (MQ=255)
                                            aaCCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGAt                                                                                                          >  7:185706/1‑149 (MQ=255)
                                              ccAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATc                                                                                                         >  6:315901/1‑148 (MQ=255)
                                              ccAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATc                                                                                                         >  6:201134/1‑148 (MQ=255)
                                              ccAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATc                                                                                                         >  1:256847/1‑148 (MQ=255)
                                               cAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCg                                                                                                        >  1:326884/1‑148 (MQ=255)
                                                aGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCtt                                                                                                                               >  4:292288/1‑124 (MQ=255)
                                                aGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCtt                                                                                                                               <  3:292288/124‑1 (MQ=255)
                                                aGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGaa                                                                                                      <  6:344542/149‑1 (MQ=255)
                                                 ggCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                                  >  1:51806/1‑136 (MQ=255)
                                                 ggCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                                  <  2:51806/136‑1 (MQ=255)
                                                    aTTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGc                                                                                                  <  1:218185/149‑1 (MQ=255)
                                                          gACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCacac                                                                                            >  1:67587/1‑149 (MQ=255)
                                                           aCGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCacac                                                                                            <  2:67587/148‑1 (MQ=255)
                                                             gATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGc                                                                                         <  5:243061/149‑1 (MQ=255)
                                                                   gggAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGc                                                                                         <  7:34236/143‑1 (MQ=255)
                                                                   gggAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGc                                                                                         >  8:34236/1‑143 (MQ=255)
                                                                   gggAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTc                                                                                   <  5:201134/149‑1 (MQ=255)
                                                                    ggAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCg                                                                                  >  2:117994/1‑149 (MQ=255)
                                                                      aGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGTATCGAACTGCTCACACTGCACGCTCGGc                                                                                <  2:312258/149‑1 (MQ=255)
                                                                         gcctgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                                  <  6:367614/112‑1 (MQ=255)
                                                                         gcctgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGaa                                                                                                                  >  5:367614/1‑112 (MQ=255)
                                                                         gcctgcctACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGc                                                                             <  5:188981/149‑1 (MQ=255)
                                                                                tACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAAATAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACt                                                                      >  4:29361/1‑149 (MQ=255)
                                                                                  ccccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCg                                                                    <  5:218871/149‑1 (MQ=255)
                                                                                  ccccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCg                                                                    <  2:256847/149‑1 (MQ=255)
                                                                                    ccGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGc                                                                  >  6:43204/1‑149 (MQ=255)
                                                                                     cGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCt                                                                                                 >  4:4214/1‑117 (MQ=255)
                                                                                     cGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCt                                                                                                 <  3:4214/117‑1 (MQ=255)
                                                                                         cACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGa                                                                                                                             <  3:166623/85‑1 (MQ=255)
                                                                                         cACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGa                                                                                                                             >  4:166623/1‑85 (MQ=255)
                                                                                          aCTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTgcg                                                             >  4:94561/1‑148 (MQ=255)
                                                                                            ttCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAg                                                          >  3:101549/1‑149 (MQ=255)
                                                                                                   tGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGAt                                                  >  8:9581/1‑149 (MQ=255)
                                                                                                          aTACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTg                                           <  5:43204/149‑1 (MQ=255)
                                                                                                              ttCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGt                                       <  8:7450/148‑1 (MQ=255)
                                                                                                                 gAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGaa                                    >  5:354367/1‑149 (MQ=255)
                                                                                                                  aGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAAt                                   <  6:176253/149‑1 (MQ=255)
                                                                                                                   gCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATg                                  <  5:40638/149‑1 (MQ=255)
                                                                                                                    cGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGc                                 >  2:295579/1‑149 (MQ=255)
                                                                                                                     gTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCg                                >  7:176430/1‑149 (MQ=255)
                                                                                                                       atcatcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCg                                                                    <  7:321219/112‑1 (MQ=255)
                                                                                                                       atcatcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCg                                                                    >  8:321219/1‑112 (MQ=255)
                                                                                                                       atcatcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGa                               >  6:356681/1‑148 (MQ=255)
                                                                                                                           tcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCAt                                                                           >  8:304930/1‑101 (MQ=255)
                                                                                                                           tcCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCAt                                                                           <  7:304930/101‑1 (MQ=255)
                                                                                                                              aCCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAGACGGGGATGTGGTTGCGGTGAATGCGACAGTGc                         <  1:231281/148‑1 (MQ=255)
                                                                                                                              aCCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGc                         >  2:231281/1‑147 (MQ=255)
                                                                                                                              aCCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCaa                       >  8:90226/1‑149 (MQ=255)
                                                                                                                               ccGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGc                                                                  >  3:180296/1‑106 (MQ=255)
                                                                                                                               ccGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGc                                                                  <  4:180296/106‑1 (MQ=255)
                                                                                                                                      ccATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGc                         >  8:200924/1‑139 (MQ=255)
                                                                                                                                      ccATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGc                         <  7:200924/139‑1 (MQ=255)
                                                                                                                                        aTCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCgg                                        >  6:29035/1‑122 (MQ=255)
                                                                                                                                        aTCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCgg                                        <  5:29035/122‑1 (MQ=255)
                                                                                                                                        aTCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATc             >  4:63304/1‑149 (MQ=255)
                                                                                                                                         tCGAATGCCGCTGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATcc            >  8:267742/1‑149 (MQ=255)
                                                                                                                                         tCGAATGCCGCTGCATTCGACGAGGACCAGCGATTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGAGCAG‑CGGGGATGTTGTTGCGGTGAATCCGACAGGGAACCTGCGCTATcc            >  8:242104/1‑149 (MQ=255)
                                                                                                                                           gAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCcg          <  4:101549/149‑1 (MQ=255)
                                                                                                                                                cAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTGGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGtt     <  2:326884/149‑1 (MQ=255)
                                                                                                                                                   cGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGTTGTc  <  5:356681/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CAGGGAATCAGTCTCGTACGCCACACAGCAAGGAGCGCAGCATGAACCAGGCATTACTGACGATCACGGGAGGGCCTGCCTACCCCGTGCACTTCTCGCTGGTCGAATACATCGAGCGTATCATCCACCGCTACCCATCGAATGCAGCGGCATTCGACGAGGACCAGCGCTTGACCTACAGCGAACTAAGGATCGAACTGCTCACACTGCACGCTCGGCTGCATGAACTCGGCGTGCGCAG‑CGGGGATGTGGTTGCGGTGAATGCGACAGTGCAACTGCGCTATCCCGTCGTTGTC  >  NC_002947/4315877‑4316172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑