Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,360,762 G→A 7.7% G120G (GGC→GGT) ‡ PP_3835 ← two‑component system sensor histidine kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,360,7620GA 7.7% 150.4 / 2.6 52G120G (GGC→GGT) ‡PP_3835two‑component system sensor histidine kinase
Reads supporting (aligned to +/- strand):  ref base G (26/22);  new base A (2/2);  total (28/24)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01

TCAACATGTTGTTGAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATCCG  >  NC_002947/4360614‑4360910
                                                                                                                                                    |                                                                                                                                                    
tCAACATGTTGTTGAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTggccagg                                                                                                                                                      >  6:8229/1‑149 (MQ=255)
  aaCATGTTGTTGAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTggccaggc                                                                                                                                                     >  1:196566/1‑148 (MQ=255)
  aaCATGTTGTTGAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTggccaggc                                                                                                                                                     <  6:348743/148‑1 (MQ=255)
       gttgttGAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGa                                                                                                                                               >  5:242804/1‑149 (MQ=255)
          gttgAAGTCGTGCGCCAGACCAGCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATc                                                                                                                                             <  6:242804/148‑1 (MQ=255)
          gttgAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTggccaggcc                                                                                                                                                    >  5:242651/1‑141 (MQ=255)
          gttgAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTggccaggcc                                                                                                                                                    <  6:242651/141‑1 (MQ=255)
           ttgAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCt                                                                                                                                            <  3:346953/148‑1 (MQ=255)
           ttgAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTg                                                                                                                                           >  8:158526/1‑149 (MQ=255)
                 tCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGATCATTGGCCTGGCCaag                                                                                                                                     <  7:264338/149‑3 (MQ=255)
                      cgcCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTtca                                                                                                                                 <  4:299952/148‑1 (MQ=255)
                       gcCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTtcatc                                                                                                                               >  6:336583/1‑149 (MQ=255)
                       gcCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTtcat                                                                                                                                >  2:345938/1‑148 (MQ=255)
                              caccGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTTGCCACTTTCATCACGGATc                                                                                                                        <  5:8229/149‑1 (MQ=255)
                              caccGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATc                                                                                                                        <  2:196566/149‑1 (MQ=255)
                                        cTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTgg                                                                                                                                          <  1:145143/121‑1 (MQ=255)
                                        cTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTg                                                                                                              >  2:278698/1‑149 (MQ=255)
                                        cTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCAgg                                                                                                                                          >  2:145143/1‑121 (MQ=255)
                                             cGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTgccgc                                                                                                          >  7:240149/1‑148 (MQ=255)
                                                  gCTTGAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGc                                                                                                    <  4:111108/149‑1 (MQ=255)
                                                    ttCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATca                                                                                                                       >  2:331289/1‑128 (MQ=255)
                                                    ttCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATca                                                                                                                       <  1:331289/128‑1 (MQ=255)
                                                         gCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAAc                                                                                             >  4:243649/1‑149 (MQ=255)
                                                         gCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAAc                                                                                             >  3:290597/1‑149 (MQ=255)
                                                           ttCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTtcatca                                                                                                                              <  5:296277/114‑1 (MQ=255)
                                                           ttCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTtcatca                                                                                                                              >  6:296277/1‑114 (MQ=255)
                                                                cgcCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTg                                                                                       <  1:345938/148‑1 (MQ=255)
                                                                cgcCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTg                                                                                       <  8:220657/148‑1 (MQ=255)
                                                                cgcCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGc                                                                                      <  3:243649/149‑1 (MQ=255)
                                                                      gAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCt                                                                                >  2:329496/1‑149 (MQ=255)
                                                                        aCAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTg                                                                              >  7:327291/1‑149 (MQ=255)
                                                                         cAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCTTCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTgc                                                                             <  1:278698/149‑1 (MQ=255)
                                                                            cTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCa                                                                          >  6:109596/1‑149 (MQ=255)
                                                                                      aCTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCACCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGcc                                                                >  5:148083/1‑149 (MQ=255)
                                                                                           gTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGt                                                           <  8:327291/149‑1 (MQ=255)
                                                                                            tCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTc                                                          <  8:240149/149‑1 (MQ=255)
                                                                                                   gctgcGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTgcgc                                                    >  2:144244/1‑148 (MQ=255)
                                                                                                                gcgACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTc                                        <  5:109596/147‑1 (MQ=255)
                                                                                                                gcgACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCa                                      <  3:83128/149‑1 (MQ=255)
                                                                                                                  gACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAgc                                    >  3:256603/1‑149 (MQ=255)
                                                                                                                     ggTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGcc                                 <  4:290597/149‑1 (MQ=255)
                                                                                                                     ggTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGcc                                 >  3:160668/1‑149 (MQ=255)
                                                                                                                       tCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCa                                                                   >  5:220990/1‑113 (MQ=255)
                                                                                                                       tCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCa                                                                   <  6:220990/113‑1 (MQ=255)
                                                                                                                       tCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCa                                                                   >  1:315326/1‑113 (MQ=255)
                                                                                                                       tCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCa                                                                   <  2:315326/113‑1 (MQ=255)
                                                                                                                          gTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGca                            >  2:227266/1‑149 (MQ=255)
                                                                                                                                tCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCAcagca                       <  5:336583/148‑1 (MQ=255)
                                                                                                                                tCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCAcag                         >  1:186918/1‑146 (MQ=255)
                                                                                                                                tCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCAcag                         >  2:276186/1‑146 (MQ=255)
                                                                                                                                         aCCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCCCAgg             >  7:271791/1‑149 (MQ=255)
                                                                                                                                          ccTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAggg            >  4:151808/1‑149 (MQ=255)
                                                                                                                                          aagtGGCCAGATCATTGGCCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAggg            >  8:45075/4‑149 (MQ=255)
                                                                                                                                           cTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGc                                                                                          >  2:15152/1‑70 (MQ=255)
                                                                                                                                           cTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGc                                                                                          <  1:15152/70‑1 (MQ=255)
                                                                                                                                            gtGGCCAGATCATTGGCCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCg                                                            <  3:319578/98‑1 (MQ=255)
                                                                                                                                            gtGGCCAGATCATTGGCCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCg                                                            >  4:319578/2‑99 (MQ=255)
                                                                                                                                              ggccaggccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGggcg          <  1:144244/147‑1 (MQ=255)
                                                                                                                                                ccaggccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCa      >  7:148001/1‑149 (MQ=255)
                                                                                                                                                  aggccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATc    >  3:38145/1‑149 (MQ=255)
                                                                                                                                                  aggccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATc    >  6:125111/1‑149 (MQ=255)
                                                                                                                                                  aggccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACACGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATc    >  8:300775/1‑149 (MQ=255)
                                                                                                                                                    gccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATCCg  >  8:324382/1‑149 (MQ=255)
                                                                                                                                                    gccaATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGAGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATCCg  >  2:141157/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TCAACATGTTGTTGAAGTCGTGCGCCAGACCACCGGTCAACTGGCCGAGGGCTTCAAGCTTCTGCGCCTGGAACAGCTGGGCGCGCACTGCGTCGAGCTGCTGCGCCGTCTCGCGACGGTCAGTGATATCACGTGTCACCTTGGCCAGGCCAATGATCTGGCCACTTTCATCACGGATCACATCCAGTGCCGCCAAGGCCCAGAACTGCGTGCCGTCCTTGCGCACCCGCCAGCCTTCGTCCTGCGCCACACCTTGCTCCAGCGCCTGGCACAGCAGACGCTCAGGGCGGCCATCCG  >  NC_002947/4360614‑4360910

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑