Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,445,250 C→G 13.7% G117A (GGG→GCG) ‡ nicE ← maleate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,445,2500CG13.7% 126.5 / 13.4 51G117A (GGG→GCG) ‡nicEmaleate isomerase
Reads supporting (aligned to +/- strand):  ref base C (21/23);  new base G (4/3);  total (25/26)
Fisher's exact test for biased strand distribution p-value = 7.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.12e-01

GGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAG  >  NC_002947/4445105‑4445398
                                                                                                                                                 |                                                                                                                                                    
ggggATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCgg                                                                                                                                                   >  6:182649/1‑149 (MQ=255)
        ccAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTg                                                                                                                                           <  7:322237/149‑1 (MQ=255)
         cAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcg                                                                                                                                          <  1:141408/149‑3 (MQ=255)
         cAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTg                                                                                                                                           >  8:322237/1‑148 (MQ=255)
                 gcCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCgcc                                                                                                                                  >  3:226018/1‑147 (MQ=255)
                   cAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGc                                                                                                                                <  4:15205/149‑1 (MQ=255)
                        cTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTg                                                                                                                           >  6:28214/1‑149 (MQ=255)
                            aCCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGcatc                                                                                                                       <  5:28214/149‑1 (MQ=255)
                               tCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCAtt                                                                                                                    >  7:32747/1‑149 (MQ=255)
                                      cTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTcggcg                                                                                                             <  1:49270/149‑1 (MQ=255)
                                          cTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTg                                                                                                          <  1:323835/148‑1 (MQ=255)
                                           tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGt                                                                                                                                  >  5:46723/1‑123 (MQ=255)
                                           tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGt                                                                                                                                  <  6:46723/123‑1 (MQ=255)
                                           tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTg                                                                                                          >  1:354678/1‑147 (MQ=255)
                                           tCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTg                                                                                                          <  2:354678/147‑1 (MQ=255)
                                                      gTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGgc                                                                                             <  1:259152/149‑1 (MQ=255)
                                                            caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCtttt                                                                                       <  5:182649/149‑1 (MQ=255)
                                                            caccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCttt                                                                                        >  3:42253/1‑148 (MQ=255)
                                                              ccaACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCtttt                                                                                       >  1:12640/1‑147 (MQ=255)
                                                                aaCTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCtttt                                                                                       >  4:59782/1‑145 (MQ=255)
                                                                  cTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCctgc                                                                                                    >  7:330980/1‑130 (MQ=255)
                                                                  cTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCctgc                                                                                                    <  8:330980/130‑1 (MQ=255)
                                                                   tCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                         <  8:211478/92‑4 (MQ=255)
                                                                   tCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                         <  8:205974/92‑4 (MQ=255)
                                                                   tCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                         >  7:211478/1‑89 (MQ=255)
                                                                   tCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCGGGGCGCTGGtcga                                                                                                                                         >  7:205974/1‑89 (MQ=255)
                                                                    cGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTgg                                                                               <  8:320961/149‑1 (MQ=255)
                                                                         aGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGt                                                                           >  8:283233/1‑148 (MQ=255)
                                                                              cTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagc                                                                     <  4:42253/149‑1 (MQ=255)
                                                                                  aTGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTgg                                                                               <  4:356971/135‑1 (MQ=255)
                                                                                  aTGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTgg                                                                               >  3:356971/1‑135 (MQ=255)
                                                                                  aTGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATagccca                                                                  <  1:24551/148‑1 (MQ=255)
                                                                                      aCGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                      <  5:28123/124‑1 (MQ=255)
                                                                                      aCGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTc                                                                                      >  6:28123/1‑124 (MQ=255)
                                                                                         gcgcGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATg                                                          <  7:283233/149‑1 (MQ=255)
                                                                                          cgcgACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATgg                                                         >  5:146671/1‑149 (MQ=255)
                                                                                           gcgACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGc                                                        >  5:25136/1‑149 (MQ=255)
                                                                                                 aTGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGAt                                                  >  5:22987/1‑149 (MQ=255)
                                                                                                                gCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCACTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAaggcaggc                                   <  8:50434/149‑1 (MQ=255)
                                                                                                                     catcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAg                                                                                               <  6:199539/84‑1 (MQ=255)
                                                                                                                     catcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAg                                                                                               >  5:199539/1‑84 (MQ=255)
                                                                                                                       tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGccc                            <  6:22987/149‑1 (MQ=255)
                                                                                                                       tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGccc                            <  3:59782/149‑1 (MQ=255)
                                                                                                                       tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGcc                             >  4:301792/1‑148 (MQ=255)
                                                                                                                       tcaCATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGcc                             >  2:357670/1‑148 (MQ=255)
                                                                                                                        cacaTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGcc                             >  2:265874/1‑147 (MQ=255)
                                                                                                                         acaTGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCa                           <  6:146671/148‑1 (MQ=255)
                                                                                                                               aaGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATc                    <  6:25136/149‑1 (MQ=255)
                                                                                                                                         ccAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGAAGGCATAGCCCAGCACATCGACCTTGGCg          >  2:269380/1‑149 (MQ=255)
                                                                                                                                          cAGCGCCGGGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGt         >  2:109778/1‑149 (MQ=255)
                                                                                                                                             ggcCCCGGCGCTGGTGATAACCGGTGCCAGTGCTTGATTGTCGGCGGTGGCCTGCGGCAGGGGCTTTTCAGATTGGCGTTGATAGCCCTGCCCGAGGGCCATGATCGGCAGAAGGCTGGCATAGCCCAGCACATCGACCTTGGCGTCgg      <  5:287653/148‑1 (MQ=255)
                                                                                                                                               ccccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGAGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGa     >  5:190790/1‑148 (MQ=255)
                                                                                                                                                cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGa     >  4:60433/1‑147 (MQ=255)
                                                                                                                                                cccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGa     >  3:71934/1‑147 (MQ=255)
                                                                                                                                                 ccGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAg  >  7:113437/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GGGGATTTCCAGCGCGCGCCAGTCCTGCACCTCGAAGCCTTCCTCGCGGATGTAGTTCACCACCAACTCGGTCAGCGGCTTCATGTACGGCGCGACGATGGCAATGCGCTTGGCCTTCATCACATGCAAGGCCTCGACCAGCGCCCCGGCGCTGGTGATCACCGGTGCCAGTGCATCATTGTCGGCGGTGGCCTGCTGCAGGCGCTTTTCAGATTGGCGGTGATAGCCCAGCCCCATGGCCATGATCGCCACAAGGCAGGCATAGCCCAGCACATCGACCTTGGCGTCGGACAG  >  NC_002947/4445105‑4445398

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑