Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,474,687	G→C	5.5%	L283L (CTC→CTG)	PP_3964 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,474,687	0	G	C	5.5%	236.8 / 3.6	73	L283L (CTC→CTG)	PP_3964	transposase
Reads supporting (aligned to +/- strand):  ref base G (32/37);  new base C (2/2);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.65e-01

CATGCAGCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGACCAGTGG  >  NC_002947/4474540‑4474833                                                                                                                                                    |                                                                                                                                                     cATGCAGCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGa                                                                                                                                                     <  7:28021/149‑1 (MQ=255) cATGCAGCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGa                                                                                                                                                     <  3:82243/149‑1 (MQ=255) cATGCAGCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGa                                                                                                                                                     <  3:40284/149‑1 (MQ=255)     cAGCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGccc                                                                                                                                                 <  1:345338/149‑1 (MQ=255)       gCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTg                                                                                                                                               <  1:31414/149‑1 (MQ=255)            tttCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAAc                                                                                                                                          <  4:233522/149‑1 (MQ=255)            tttCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAAc                                                                                                                                          <  1:266641/149‑1 (MQ=255)                aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                      <  8:344068/149‑3 (MQ=14)                aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                      >  7:344068/1‑147 (MQ=14)                aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                      <  2:171004/149‑3 (MQ=14)                aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                      >  1:171004/1‑147 (MQ=14)                  tGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGcgt‑c                                                                                                                                   <  5:275886/149‑1 (MQ=255)                  tGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGcgt‑c                                                                                                                                   >  8:167385/1‑149 (MQ=255)                  tGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGcgt‑c                                                                                                                                   >  6:111408/1‑149 (MQ=255)                       ccTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCt                                                                                                                              <  5:97489/149‑1 (MQ=255)                           gTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGt                                                                                                                            <  5:88088/147‑1 (MQ=255)                           gTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTAc                                                                                                                          >  2:105856/1‑149 (MQ=255)                            ttCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACg                                                                                                                         >  2:294798/1‑149 (MQ=255)                                   ccGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGa                                                                                                                   >  7:244998/1‑148 (MQ=255)                                   ccGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGAc                                                                                                                  <  5:87311/149‑1 (MQ=255)                                    cGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGAGCATGTTCGCCAGCACGGCTTGGGATGAAGTCGTAAACCACAGCTTTGAGCCCAGAAAACGGCGT‑CGTGCTGTACGCCCAGAc                                                                                                                  >  4:25804/1‑148 (MQ=255)                                      gCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACa                                                                                                                 >  7:191968/1‑147 (MQ=255)                                        tttGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAgg                                                                                                             >  4:126710/1‑149 (MQ=255)                                                 aGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTggg                                                                                                    <  8:244998/149‑1 (MQ=255)                                                        tcgtcgCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttctt                                                                                             <  4:98308/149‑1 (MQ=255)                                                             gCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGc                                                                                        >  7:99393/1‑149 (MQ=255)                                                             gCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGc                                                                                        <  5:111408/149‑1 (MQ=255)                                                              cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttcttc                                                                                            <  2:14455/144‑1 (MQ=255)                                                              cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttcttc                                                                                            >  1:14455/1‑144 (MQ=255)                                                              cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttctt                                                                                             <  1:121390/143‑1 (MQ=255)                                                              cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttctt                                                                                             >  2:121390/1‑143 (MQ=255)                                                                      cTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCaa                                                                              <  3:126710/149‑1 (MQ=255)                                                                       ttGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAg                                                                             >  2:219386/1‑149 (MQ=255)                                                                           cGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGTCCGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCAt                                                                          <  1:287714/149‑1 (MQ=255)                                                                                  gTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTg                                                                       >  6:237295/1‑144 (MQ=255)                                                                                  gTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTg                                                                       <  5:237295/144‑1 (MQ=255)                                                                                      cccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGt                                                                                                       >  3:323696/1‑109 (MQ=255)                                                                                      cccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGt                                                                                                       <  4:323696/109‑1 (MQ=255)                                                                                       ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCT‑TGGCGCaa                                                                              <  8:130632/132‑1 (MQ=255)                                                                                       ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCT‑TGGCGCaa                                                                              >  7:130632/1‑132 (MQ=255)                                                                                       ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtc                                                             >  7:209647/1‑149 (MQ=255)                                                                                       ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtc                                                             >  6:144651/1‑149 (MQ=255)                                                                                       ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtc                                                             <  5:144651/149‑1 (MQ=255)                                                                                        cAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtct                                                            >  5:214102/1‑149 (MQ=255)                                                                                         aGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtct                                                            >  6:186702/1‑148 (MQ=255)                                                                                                 gcgcgcATGTTCGCCAGGACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAAGGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCTGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCg                                                    <  3:25804/148‑1 (MQ=255)                                                                                                 gcgcgcATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCg                                                    <  1:294798/148‑1 (MQ=255)                                                                                                   gcgcATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTgg                                                 <  4:113444/149‑1 (MQ=255)                                                                                                     gcATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGa                                                >  2:364168/1‑148 (MQ=255)                                                                                                       aTGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCa                                             >  7:226656/1‑149 (MQ=255)                                                                                                        tGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCAc                                            >  1:120684/1‑149 (MQ=255)                                                                                                                 gCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGAtt                                   <  7:167385/149‑1 (MQ=255)                                                                                                                  cACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGAtt                                   >  6:191422/1‑148 (MQ=255)                                                                                                                   aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTgg                                                 <  4:153091/133‑1 (MQ=255)                                                                                                                   aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTgg                                                 >  3:153091/1‑133 (MQ=255)                                                                                                                   aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCa                                 <  8:191968/149‑1 (MQ=255)                                                                                                                   aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCa                                 <  8:209647/149‑1 (MQ=255)                                                                                                                     ggCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAg                                >  1:224079/1‑148 (MQ=255)                                                                                                                        ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGAt                                               <  1:112903/130‑1 (MQ=255)                                                                                                                        ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGAt                                               >  2:112903/1‑130 (MQ=255)                                                                                                                        ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACG                            >  7:229783‑M1/1‑149 (MQ=255)                                                                                                                        ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACG                            >  4:243773‑M1/1‑149 (MQ=255)                                                                                                                           ggCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCT                         >  6:328026‑M1/1‑149 (MQ=255)                                                                                                                              tGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCCTTAGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTC                       <  1:105856‑M1/149‑1 (MQ=255)                                                                                                                                     gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGc                                          >  4:29638/1‑122 (MQ=255)                                                                                                                                     gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGc                                          <  3:29638/122‑1 (MQ=255)                                                                                                                                     gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGt                <  6:214102/148‑1 (MQ=255)                                                                                                                                     gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTg               >  6:195377/1‑149 (MQ=255)                                                                                                                                         accaccGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATc           <  1:364168/149‑1 (MQ=255)                                                                                                                                            accGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGa                                     >  4:147128/1‑120 (MQ=255)                                                                                                                                            accGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGa                                     <  3:147128/120‑1 (MQ=255)                                                                                                                                            accGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGAc        <  3:243773/149‑1 (MQ=255)                                                                                                                                                   tGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGACCAGTgg  <  2:224079/148‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CATGCAGCCGATTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGACCAGTGG  >  NC_002947/4474540‑4474833

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑