Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,474,698	A→G	5.9%	F280L (TTT→CTT)	PP_3964 ←	transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,474,698	0	A	G	5.9%	210.7 / 2.3	68	F280L (TTT→CTT)	PP_3964	transposase
Reads supporting (aligned to +/- strand):  ref base A (32/32);  new base G (2/2);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.32e-01

TTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGACCAGTGGCTGAAGCCGCACG  >  NC_002947/4474551‑4474846                                                                                                                                                    |                                                                                                                                                       tttCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAAc                                                                                                                                                       <  4:233522/149‑1 (MQ=255) tttCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAAc                                                                                                                                                       <  1:266641/149‑1 (MQ=255)     aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                                   <  8:344068/149‑3 (MQ=14)     aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                                   >  7:344068/1‑147 (MQ=14)     aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                                   >  1:171004/1‑147 (MQ=14)     aGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGTTTTCAGGGCTCAAAGCggtg                                                                                                                                                   <  2:171004/149‑3 (MQ=14)       tGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGcgt‑c                                                                                                                                                <  5:275886/149‑1 (MQ=255)       tGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGcgt‑c                                                                                                                                                >  8:167385/1‑149 (MQ=255)       tGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGcgt‑c                                                                                                                                                >  6:111408/1‑149 (MQ=255)            ccTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCt                                                                                                                                           <  5:97489/149‑1 (MQ=255)                gTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGt                                                                                                                                         <  5:88088/147‑1 (MQ=255)                gTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTAc                                                                                                                                       >  2:105856/1‑149 (MQ=255)                 ttCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACg                                                                                                                                      >  2:294798/1‑149 (MQ=255)                        ccGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGa                                                                                                                                >  7:244998/1‑148 (MQ=255)                        ccGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGAc                                                                                                                               <  5:87311/149‑1 (MQ=255)                         cGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGAGCATGTTCGCCAGCACGGCTTGGGATGAAGTCGTAAACCACAGCTTTGAGCCCAGAAAACGGCGT‑CGTGCTGTACGCCCAGAc                                                                                                                               >  4:25804/1‑148 (MQ=255)                           gCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACa                                                                                                                              >  7:191968/1‑147 (MQ=255)                             tttGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAgg                                                                                                                          >  4:126710/1‑149 (MQ=255)                                      aGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTggg                                                                                                                 <  8:244998/149‑1 (MQ=255)                                             tcgtcgCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttctt                                                                                                          <  4:98308/149‑1 (MQ=255)                                                  gCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGc                                                                                                     <  5:111408/149‑1 (MQ=255)                                                  gCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGc                                                                                                     >  7:99393/1‑149 (MQ=255)                                                   cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttcttc                                                                                                         >  1:14455/1‑144 (MQ=255)                                                   cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttcttc                                                                                                         <  2:14455/144‑1 (MQ=255)                                                   cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttctt                                                                                                          <  1:121390/143‑1 (MQ=255)                                                   cAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTttctt                                                                                                          >  2:121390/1‑143 (MQ=255)                                                           cTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCaa                                                                                           <  3:126710/149‑1 (MQ=255)                                                            ttGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAg                                                                                          >  2:219386/1‑149 (MQ=255)                                                                cGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGTCCGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCAt                                                                                       <  1:287714/149‑1 (MQ=255)                                                                       gTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTg                                                                                    <  5:237295/144‑1 (MQ=255)                                                                       gTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTg                                                                                    >  6:237295/1‑144 (MQ=255)                                                                           cccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGt                                                                                                                    >  3:323696/1‑109 (MQ=255)                                                                           cccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGt                                                                                                                    <  4:323696/109‑1 (MQ=255)                                                                            ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCT‑TGGCGCaa                                                                                           >  7:130632/1‑132 (MQ=255)                                                                            ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCT‑TGGCGCaa                                                                                           <  8:130632/132‑1 (MQ=255)                                                                            ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtc                                                                          <  5:144651/149‑1 (MQ=255)                                                                            ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtc                                                                          >  7:209647/1‑149 (MQ=255)                                                                            ccAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtc                                                                          >  6:144651/1‑149 (MQ=255)                                                                             cAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtct                                                                         >  5:214102/1‑149 (MQ=255)                                                                              aGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGtct                                                                         >  6:186702/1‑148 (MQ=255)                                                                                      gcgcgcATGTTCGCCAGGACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAAGGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCTGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCg                                                                 <  3:25804/148‑1 (MQ=255)                                                                                      gcgcgcATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCg                                                                 <  1:294798/148‑1 (MQ=255)                                                                                        gcgcATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTgg                                                              <  4:113444/149‑1 (MQ=255)                                                                                          gcATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGa                                                             >  2:364168/1‑148 (MQ=255)                                                                                            aTGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCa                                                          >  7:226656/1‑149 (MQ=255)                                                                                             tGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCAc                                                         >  1:120684/1‑149 (MQ=255)                                                                                                      gCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGAtt                                                <  7:167385/149‑1 (MQ=255)                                                                                                       cACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGAtt                                                >  6:191422/1‑148 (MQ=255)                                                                                                        aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTgg                                                              >  3:153091/1‑133 (MQ=255)                                                                                                        aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTgg                                                              <  4:153091/133‑1 (MQ=255)                                                                                                        aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCa                                              <  8:191968/149‑1 (MQ=255)                                                                                                        aCGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCa                                              <  8:209647/149‑1 (MQ=255)                                                                                                          ggCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAg                                             >  1:224079/1‑148 (MQ=255)                                                                                                             ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGAt                                                            <  1:112903/130‑1 (MQ=255)                                                                                                             ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGAt                                                            >  2:112903/1‑130 (MQ=255)                                                                                                             ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACG                                         >  7:229783‑M1/1‑149 (MQ=255)                                                                                                             ttGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACG                                         >  4:243773‑M1/1‑149 (MQ=255)                                                                                                                ggCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCT                                      >  6:328026‑M1/1‑149 (MQ=255)                                                                                                                   tGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCCTTAGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTC                                    <  1:105856‑M1/149‑1 (MQ=255)                                                                                                                          gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGc                                                       >  4:29638/1‑122 (MQ=255)                                                                                                                          gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGc                                                       <  3:29638/122‑1 (MQ=255)                                                                                                                          gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGt                             <  6:214102/148‑1 (MQ=255)                                                                                                                          gTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTg                            >  6:195377/1‑149 (MQ=255)                                                                                                                              accaccGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATc                        <  1:364168/149‑1 (MQ=255)                                                                                                                                 accGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGa                                                  >  4:147128/1‑120 (MQ=255)                                                                                                                                 accGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGa                                                  <  3:147128/120‑1 (MQ=255)                                                                                                                                 accGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGAc                     <  3:243773/149‑1 (MQ=255)                                                                                                                                        tGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGACCAGTgg               <  2:224079/148‑1 (MQ=255)                                                                                                                                                    aCGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCAgccactggtcgatgcactgcgtgaagccgtgct  <  5:191422‑M1/149‑34 (MQ=255)                                                                                                                                                    |                                                                                                                                                       TTTCAGTGATGCCTTGTTCGAAACCGGCTTTGTAGCCAGCGAAGTCGTCGCAGACCAACTTGCCGTTCCAGTCACCCAGGAAGTTGCGCGCATGTTCGCCAGCACGGCTTGGGCTGAAGTCGTAAACCACCGCTTTGAGCCCTGAAAACGGCGT‑CGTGCTGTACGCCCAGACATAGGCCCGGTGGGTTTTCTTCTCGCC‑TGGCGCAAGCATTTGCACCGGTGTCTCATCAGCGTGGATCACGCCCTGATTCAGCACGGCTTCACGCAGTGCATCGACCAGTGGCTGAAGCCGCACG  >  NC_002947/4474551‑4474846

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑