Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,509,767 T→C 6.0% I99V (ATC→GTC)  serS ← serine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,509,7670TC 6.0% 246.2 / 4.0 83I99V (ATC→GTC) serSserine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (39/39);  new base C (2/3);  total (41/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCT  >  NC_002947/4509621‑4509913
                                                                                                                                                  |                                                                                                                                                  
aCCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGACGc                                                                                                                                                  >  6:265021/1‑149 (MQ=255)
aCCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGACGc                                                                                                                                                  >  6:270019/1‑149 (MQ=255)
  cGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCa                                                                                                                                                >  2:211386/1‑149 (MQ=255)
  cGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCa                                                                                                                                                >  8:202526/1‑149 (MQ=255)
    cTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTACGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATc                                                                                                                                              >  1:274242/1‑149 (MQ=255)
       aTTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATcc                                                                                                                                             <  3:132627/147‑1 (MQ=255)
        tttCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCGCAAGGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACGGGCACTCTTGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCAt                                                                                                                                               <  3:92175/144‑1 (MQ=255)
        tttCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGACCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGACCGGCAGGTTGGGAATGGTCAGCAGGATGCCAt                                                                                                                                               >  4:92175/1‑144 (MQ=255)
                    ccACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGc                                                                                                                                     >  1:323002/1‑142 (MQ=255)
                    ccACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGc                                                                                                                                     <  2:323002/142‑1 (MQ=255)
                    ccACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATg                                                                                                                              >  4:353787/1‑149 (MQ=255)
                    ccACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATg                                                                                                                              <  6:150301/149‑1 (MQ=255)
                          ggTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTc                                                                                                                         >  4:75999/1‑148 (MQ=255)
                           gTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCa                                                                                                                       <  7:50733/149‑1 (MQ=255)
                           gTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGACGCCATTCAGGCCGAGCTGGATGGCATcc                                                                                                                        <  5:265021/148‑1 (MQ=255)
                           gTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGACGCCATTCAGGCCGAGCTGGATGGCATcc                                                                                                                        <  5:270019/148‑1 (MQ=255)
                                  acgccGAAGGCGTTCGACTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGc                                                                                                                >  7:91526/5‑149 (MQ=255)
                                  aTTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAg                                                                                                                 <  8:232781/148‑1 (MQ=255)
                                   tttCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCTGGACGCCATTCAGGCCGAGCTGGATGGCATCCTGCtgacca                                                                                                               <  8:99214/149‑6 (MQ=37)
                                   tttCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGcc                                                                                                               <  7:202526/149‑1 (MQ=255)
                                    ttCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGcc                                                                                                               <  2:316541/148‑1 (MQ=255)
                                       gAAGTCGAACGCCTTCGGAGTGCCCCAACGGCGCACTTCGACGTTCTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGGAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGc                                                                                                           >  1:29140/1‑149 (MQ=255)
                                        aaGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGcc                                                                                                          <  6:251643/149‑1 (MQ=255)
                                             gAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAg                                                                                                     >  4:86282/1‑149 (MQ=255)
                                             gAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAg                                                                                                     >  1:156814/1‑149 (MQ=255)
                                              aaCGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGc                                                                                                    <  2:265327/149‑1 (MQ=255)
                                              aaCGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGc                                                                                                    <  1:211386/149‑1 (MQ=255)
                                               aCGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGc                                                                                                    >  1:156399/1‑148 (MQ=255)
                                                  ccTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGt                                                                                                <  3:353787/149‑1 (MQ=255)
                                                   cTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGc                                                                                                           >  4:58823/1‑137 (MQ=255)
                                                   cTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGc                                                                                                           <  3:58823/137‑1 (MQ=255)
                                                   cTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGt                                                                                                >  6:55058/1‑148 (MQ=255)
                                                    ttCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTc                                                                                              >  8:30623/1‑149 (MQ=255)
                                                      cGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGt                                                                                            >  2:327914/1‑149 (MQ=255)
                                                       ggCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGtt                                                                                           <  8:278841/149‑1 (MQ=255)
                                                           tGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGcc                                                                                       <  1:327914/149‑1 (MQ=255)
                                                              cccAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCAt                                                                                     >  8:124743/1‑148 (MQ=255)
                                                              cccAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCAt                                                                                     <  7:124743/148‑1 (MQ=255)
                                                              cccAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATg                                                                                    >  2:366619/1‑149 (MQ=255)
                                                                   cGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTc                                                                               <  5:55058/149‑1 (MQ=255)
                                                                      cgcACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGAc                                                                            >  1:208711/1‑149 (MQ=255)
                                                                      cgcACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGAc                                                                            <  2:156399/149‑1 (MQ=255)
                                                                           ttCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg                                                                       >  5:319545/1‑149 (MQ=255)
                                                                           ttCGACGTTGTCGTCTTCACTGGAGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg                                                                       >  5:193755/1‑149 (MQ=255)
                                                                             cGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAg                                                                       <  2:274242/147‑1 (MQ=255)
                                                                             cGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGc                                                                      <  7:30623/148‑1 (MQ=255)
                                                                             cGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGc                                                                      >  1:18270/1‑148 (MQ=255)
                                                                             cGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGc                                                                      >  3:241102/1‑148 (MQ=255)
                                                                              gACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCa                                                                    >  3:332962/1‑149 (MQ=255)
                                                                                cGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCAt                                                                   >  4:299294/1‑148 (MQ=255)
                                                                                 gTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCa                                                                 <  8:61176/149‑1 (MQ=255)
                                                                                    gtcgtcTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGc                                                               <  3:75999/148‑1 (MQ=255)
                                                                                       gtcTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCgggg                                                           <  3:299294/149‑1 (MQ=255)
                                                                                          ttCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAAt                                                                                                                               <  1:160846/78‑1 (MQ=255)
                                                                                          ttCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAAt                                                                                                                               >  2:160846/1‑78 (MQ=255)
                                                                                            cACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGAt                                                       <  2:156814/148‑1 (MQ=255)
                                                                                             aCTGGCCCCGACCGGCACGCTGGCGTCCTCCAGGTTGTGAATGGTCACCAGGATGCCATCCAGCACGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATCACCTCGACGTCAGCCATCAGCGGGGCGATGt                                                     >  4:149705/1‑149 (MQ=255)
                                                                                                   gcCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGc                                                          >  5:277623/1‑138 (MQ=255)
                                                                                                   gcCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGc                                                          <  6:277623/138‑1 (MQ=255)
                                                                                                           gCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttggcc                                       >  4:19143/1‑149 (MQ=255)
                                                                                                           gCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttggcc                                       >  4:155397/1‑149 (MQ=255)
                                                                                                            cACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCccttggcct                                      <  3:19143/149‑1 (MQ=255)
                                                                                                               gCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTc                                                                         >  6:159429/1‑111 (MQ=255)
                                                                                                               gCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTc                                                                         <  5:159429/111‑1 (MQ=255)
                                                                                                                cTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCcttggccttggc                                  <  4:136066/149‑1 (MQ=255)
                                                                                                                 tGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCttggccttggcc                                 >  8:290988/1‑149 (MQ=255)
                                                                                                                 tGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCttggccttggcc                                 >  2:28886/1‑149 (MQ=255)
                                                                                                                      tCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTtggcctggcc                            >  6:170581/1‑149 (MQ=255)
                                                                                                                         ggCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGAt                         <  2:29140/149‑1 (MQ=255)
                                                                                                                          gCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATg                        <  5:170581/149‑1 (MQ=255)
                                                                                                                          gCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATg                        >  8:196399/1‑149 (MQ=255)
                                                                                                                              gTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCggg                                                            >  1:104524/1‑109 (MQ=255)
                                                                                                                              gTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCggg                                                            <  2:104524/109‑1 (MQ=255)
                                                                                                                              gTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACt                    >  3:174109/1‑149 (MQ=255)
                                                                                                                              gTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACt                    >  8:110672/1‑149 (MQ=255)
                                                                                                                               ttGGGTATGGTCAGCAGGTTGCCATCCAGCTAGGCCTGAATGGCGTCCAGCTGAGCCTTGCCGGCTGCCAGTTCGTTGGCCATGCGCTCGTCGTCAGGCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGTCCTGGCCGTTGGACtt                   <  3:149705/149‑1 (MQ=255)
                                                                                                                               ttGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACtt                   <  7:290988/149‑1 (MQ=255)
                                                                                                                               ttGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACtt                   <  4:332962/149‑1 (MQ=255)
                                                                                                                                 gggAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTg                  <  4:174109/148‑1 (MQ=255)
                                                                                                                                 gggAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCCCCCTTGGCCTTGGCCTGGCCGATGGACTTg                  <  1:28886/148‑1 (MQ=255)
                                                                                                                                        gTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGc          <  4:241102/149‑1 (MQ=255)
                                                                                                                                         tCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCg         >  6:264604/1‑149 (MQ=255)
                                                                                                                                                gATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCt  <  7:110672/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
ACCGCTGATTTCACCGAGGGCCACATGGTCCTTGATTTCGAAGTCGAACGCCTTCGGCGTGCCCCAACGGCGCACTTCGACGTTGTCGTCTTCACTGGCGCCGACCGGCACGCTGGCGTCCGGCAGGTTGGGAATGGTCAGCAGGATGCCATCCAGCTCGGCCTGAATGGCGTCCAGCTCAGCCTTGCCGGCAGCCAGTTCGTTGGCCATGCGCTCGACGTCAGCCATCAGCGGGGCGATGTCTTCGCCCTTGGCCTTGGCCTGGCCGATGGACTTGGAACGGGCGTTACGCT  >  NC_002947/4509621‑4509913

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑