Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,538,463	G→A	6.1%	intergenic (+265/‑55)	PP_4025 → / → PP_4027	transposase/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,538,463	0	G	A	6.1%	193.6 / 2.4	66	intergenic (+265/‑55)	PP_4025/PP_4027	transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (29/32);  new base A (2/2);  total (31/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCAGCAAGTTCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGGCGCACC  >  NC_002947/4538317‑4538609                                                                                                                                                     |                                                                                                                                                   cgggatcaATTTTATGCGGCCGGTTTTCATCAACGGTGTTGC‑GGGGTTGTACGTTGGCTTATCGTGGGAAAAATGGTTCGGGCATGCCTTAACCGTTGTAACTCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAgg                                                                                                                                                   <  1:267902/143‑1 (MQ=255)     agcaAGTTCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTgc                                                                                                                                              >  2:187699/1‑149 (MQ=255)      gcaAGTTCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTgcg                                                                                                                                             >  2:348814/1‑149 (MQ=255)          gTTCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCTTCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAg                                                                                                                                         >  6:315476/1‑149 (MQ=255)           ttCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAg                                                                                                                                         >  3:355462/1‑148 (MQ=255)            tCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAgg                                                                                                                                        <  7:220244/148‑1 (MQ=255)              aTCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGaa                                                                                                                                      <  3:188915/148‑1 (MQ=255)              aTCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAAc                                                                                                                                     <  4:205620/149‑1 (MQ=255)                 cGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTg                                                                                                                                   >  1:134498/1‑148 (MQ=255)                    ccGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGGGGCAACACTGGTTCGGGCATGCCTGACCCTTTGT‑ACCCGATCATGATGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTaaa                                                                                                                               <  1:92970/149‑1 (MQ=255)                    ccGGTCTGCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTaaa                                                                                                                               <  4:52740/149‑1 (MQ=255)                       gtgtTCATC‑ACCGTGGTGCTGGCGTTGACCGCGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACa                                                                                                                             >  6:85084/1‑148 (MQ=255)                          ttCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACatat                                                                                                                          <  1:187699/148‑1 (MQ=255)                          ttCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCATTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACatat                                                                                                                          <  6:274066/148‑1 (MQ=255)                          ttCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCATTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACatat                                                                                                                          >  5:274066/1‑148 (MQ=255)                            catc‑aCCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATaa                                                                                                                        <  1:348814/148‑1 (MQ=255)                            catc‑aCCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATaa                                                                                                                        >  7:189356/1‑148 (MQ=255)                                              cGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGTGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGaa                                                                                                      <  3:26904/149‑1 (MQ=255)                                               gTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGaaa                                                                                                     >  1:230483/1‑149 (MQ=255)                                               gTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAAGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGaaa                                                                                                     >  4:306202/1‑149 (MQ=255)                                               gTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAAGGGTGAGCAAGGAACTGTACACATATACCACCTCTTATCAACGGaa                                                                                                      >  4:301242/1‑148 (MQ=255)                                                           cTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGCGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCa                                                                                         <  3:357491/149‑1 (MQ=255)                                                              aGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCaa                                                                                        <  5:322143/147‑1 (MQ=255)                                                                cGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCa                                                                                    <  6:167206/149‑1 (MQ=255)                                                                 gTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGt                                                                                                                                  <  4:281798/102‑1 (MQ=255)                                                                 gTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGt                                                                                                                                  >  3:281798/1‑102 (MQ=255)                                                                 gTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCaa                                                                                   >  5:86627/1‑149 (MQ=255)                                                                    gCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACg                                                                                 <  8:189356/148‑1 (MQ=255)                                                                     cAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGtt                                                                               <  3:27383/149‑1 (MQ=255)                                                                         acTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACctc                                                                                                                  >  7:109677/1‑110 (MQ=255)                                                                         acTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACctc                                                                                                                  <  8:109677/110‑1 (MQ=255)                                                                           tGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTcc                                                                         >  1:114531/1‑149 (MQ=255)                                                                              ttCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCGTTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTa                                                                      <  1:104251/149‑1 (MQ=255)                                                                                      ggCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATAc                                                              >  3:251368/1‑149 (MQ=255)                                                                                      ggCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATAc                                                              <  2:134498/149‑1 (MQ=255)                                                                                       gCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCCGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGACCTGTAAACATATAACACCTCTTAGCACCGGAAATATCTAAATGAAAAGCAACGTTATTTCCTTACAATATAcc                                                             >  3:246592/1‑149 (MQ=255)                                                                                        ccTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCt                                                            <  5:85084/149‑1 (MQ=255)                                                                                          tGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGt                                                                                                                                  <  6:100082/77‑1 (MQ=255)                                                                                          tGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGt                                                                                                                                  >  5:100082/1‑77 (MQ=255)                                                                                                       cccGATCATGACGCCCATCAGCCGTACTATCATTTATGCCCGCTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCa                                              <  6:215098/149‑1 (MQ=255)                                                                                                        ccGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAg                                             >  2:362152/1‑149 (MQ=255)                                                                                                                  ggCCCATCAGCCGTACTATCATTTATGCCAGTTGAAGGGTGCGCAAGGCACTGTAAACATATAACACCGCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAgg                                   <  3:301242/149‑1 (MQ=255)                                                                                                                  ggCCCATCAGCCGTACTATCATTTATGCCAGTTGAAGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAgg                                   <  3:306202/149‑1 (MQ=255)                                                                                                                      cATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGaa                                >  7:87241/1‑148 (MQ=255)                                                                                                                      cATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGaa                                >  3:209125/1‑148 (MQ=255)                                                                                                                            ccGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTAc                         <  4:355462/149‑1 (MQ=255)                                                                                                                            ccGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTAc                         <  6:86627/149‑1 (MQ=255)                                                                                                                                aCTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCAc                     >  7:299130/1‑149 (MQ=255)                                                                                                                                  tATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATAc                                                              >  2:95137/1‑106 (MQ=255)                                                                                                                                  tATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATAc                                                              <  1:95137/106‑1 (MQ=255)                                                                                                                                  tATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAgg                                   <  2:175013/133‑1 (MQ=255)                                                                                                                                  tATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAgg                                   >  1:175013/1‑133 (MQ=255)                                                                                                                                    tCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATTTAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTgg                  <  8:87241/148‑1 (MQ=255)                                                                                                                                    tCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTgg                  <  4:209125/148‑1 (MQ=255)                                                                                                                                      aTTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCCAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGt                <  2:230483/148‑1 (MQ=255)                                                                                                                                       tttATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGttt              >  7:295625/1‑149 (MQ=255)                                                                                                                                       tttATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGtt               >  1:362486/1‑148 (MQ=255)                                                                                                                                           tGCCAGTTGATGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATTCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCAc           <  4:246592/148‑1 (MQ=255)                                                                                                                                            gCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACgg         >  2:154087/1‑149 (MQ=255)                                                                                                                                             ccAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACgg         <  5:315476/148‑1 (MQ=255)                                                                                                                                              cAGTTGAGGGTTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCt                                                            <  4:21420/96‑1 (MQ=255)                                                                                                                                              cAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGgc       >  1:176761/1‑149 (MQ=255)                                                                                                                                               aGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGGAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACTGgcg      <  4:251368/149‑1 (MQ=255)                                                                                                                                                gTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGgcgc     <  8:299130/149‑1 (MQ=255)                                                                                                                                                   gAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGGCGCAcc  >  5:141347/1‑149 (MQ=255)                                                                                                                                                   gAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGGCGCAcc  >  3:210749/1‑149 (MQ=255)                                                                                                                                                   gAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGGCGCAcc  >  7:323990/1‑149 (MQ=255)                                                                                                                                                     |                                                                                                                                                   CGGCAGCAAGTTCATCCGCCCGGTGTTCATC‑ACCGTGGTGCTGGCGTTGACCGTGCGCTTAGCGTGGCAACACTGGTTCGGGCAGGCCTGACCCTTTGT‑ACCCGATCATGAGGCCCATCAGCCGTACTATCATTTATGCCAGTTGAGGGGTGCGCAAGGAACTGTAAACATATAACACCTCTTATCAACGGAAATATCTAAATGCAAAGCAACGTTATTTCCTTACAATATACCTGTTATGGCGCCTCAGCTATGCCAGGGAATATTTACGCACTGGGTTTCACGGGCGCACC  >  NC_002947/4538317‑4538609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑