Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,574,227	G→C	6.6%	R300P (CGC→CCC) ‡	glgX →	glycogen debranching enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,574,227	0	G	C	6.6%	193.6 / 4.3	61	R300P (CGC→CCC) ‡	glgX	glycogen debranching enzyme
Reads supporting (aligned to +/- strand):  ref base G (29/28);  new base C (2/2);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-01

CCGCACCCGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTGGGCCG  >  NC_002947/4574079‑4574370                                                                                                                                                     |                                                                                                                                                ccGCACCCGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATgcg                                                                                                                                                 >  7:62217/1‑149 (MQ=255)  cGCACCCGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATgcgc                                                                                                                                                <  2:187150/149‑1 (MQ=255)  cGCACCCGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCAAAGGCAACGAGCGCGGCCCGACCCTGTCGATgcgc                                                                                                                                                <  1:230372/149‑1 (MQ=255)        cgcgCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATc                                                                                                                                          <  1:152591/149‑1 (MQ=255)               ccTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCCGCGCATCGACag                                                                                                                                     <  6:335479/147‑2 (MQ=255)                cTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGAc                                                                                                                                       <  3:88959/144‑1 (MQ=255)                cTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGAc                                                                                                                                       >  4:88959/1‑144 (MQ=255)                     cAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCg                                                                                                                              >  5:30410/1‑148 (MQ=255)                         ggCAAGATCGCCGAGTTCAAGGATATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGtacta                                                                                                                         <  6:276534/149‑1 (MQ=255)                              gATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGcc                                                                                                                    >  1:196096/1‑149 (MQ=255)                                     gAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGcc                                                                                                             <  8:62217/149‑1 (MQ=255)                                             ggAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCCGCGCATCGACagg                                                                                                                                    >  4:285018/1‑116 (MQ=255)                                             ggAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCCGCGCATCGACagg                                                                                                                                    <  3:285018/118‑3 (MQ=255)                                                 aTGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTTTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAgc                                                                                                  <  6:30410/148‑1 (MQ=255)                                                  gcgTCGTGCAGGTGCGCGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAgcgc                                                                                                >  1:194377/3‑149 (MQ=255)                                                  gcgTCGTGCAGGTGCGCGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAgcgc                                                                                                >  7:83837/3‑149 (MQ=255)                                                   ggTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGa                                                                                                                                        >  6:243183/1‑108 (MQ=255)                                                   ggTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGa                                                                                                                                        <  5:243183/108‑1 (MQ=255)                                                           aCCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATc                                                                                       <  3:228922/149‑1 (MQ=255)                                                           aCCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATc                                                                                       <  5:298437/149‑1 (MQ=255)                                                             cTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGga                                                                                                          <  6:270395/128‑1 (MQ=255)                                                             cTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGga                                                                                                          >  5:270395/1‑128 (MQ=255)                                                               gCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAAc                                                                                    >  7:30894/1‑148 (MQ=255)                                                                  cgacgCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGcc                                                                                                             >  3:58758/1‑120 (MQ=255)                                                                  cgacgCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGcc                                                                                                             <  4:58758/120‑1 (MQ=255)                                                                   gacgCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTc                                                                               >  3:214240/1‑149 (MQ=255)                                                                   gacgCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTc                                                                               >  5:144247/1‑149 (MQ=255)                                                                   gacgCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTc                                                                               <  2:194377/149‑1 (MQ=255)                                                                      gcgGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTccgg                                                                            >  3:310386/1‑149 (MQ=255)                                                                        ggggCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTAACGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTccggca                                                                          >  1:173346/1‑149 (MQ=255)                                                                           gCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGAGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTAATACATCAACGACTccgccaccg                                                                       >  7:164489/1‑149 (MQ=255)                                                                               gAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGCCCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCaa                                                                   >  6:379208/1‑149 (MQ=255)                                                                                      tCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGcc                                                                                                             <  2:18675/100‑1 (MQ=255)                                                                                      tCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGcc                                                                                                             >  1:18675/1‑100 (MQ=255)                                                                                      tCCGGGACGTGGGCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTg                                                            <  4:55320/149‑1 (MQ=255)                                                                                        cTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTgg                                                           <  8:83837/148‑1 (MQ=255)                                                                                         tGGACGTGGTCTACAACCACACCGCCGAAGGCATCGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGAc                                                         >  2:100105/1‑149 (MQ=255)                                                                                         tGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGAc                                                         >  8:63913/1‑149 (MQ=255)                                                                                           gACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGAc                                                         <  7:63913/147‑1 (MQ=255)                                                                                             cGTGGTCTACAACCACACCGCCGAAGGCATCGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGa                                                     <  1:100105/149‑1 (MQ=255)                                                                                                  tCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCAc                                                <  8:30894/149‑1 (MQ=255)                                                                                                   cttcAACCACAACGCAGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGGGCTACTACATCAACGAGTCCGGCACCGGCAACACGCTGGACCTGAGCCAc                                                <  8:164489/145‑1 (MQ=255)                                                                                                   cTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCAcc                                               >  5:31243/1‑149 (MQ=255)                                                                                                      cAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCAccc                                              >  8:153561/1‑147 (MQ=255)                                                                                                      cAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCAccc                                              <  7:153561/147‑1 (MQ=255)                                                                                                       aaCCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTg                                           >  8:343975/1‑149 (MQ=255)                                                                                                         ccACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGctac                                                                                             <  5:163388/97‑1 (MQ=255)                                                                                                         ccACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGctac                                                                                             >  6:163388/1‑97 (MQ=255)                                                                                                                    aaGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTg                              <  4:310386/149‑1 (MQ=255)                                                                                                                            gAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGAc                                                         >  2:289395/1‑114 (MQ=255)                                                                                                                            gAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGAc                                                         <  1:289395/114‑1 (MQ=255)                                                                                                                              gcgcGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTccggcaccg                                                                       <  4:291583/98‑1 (MQ=255)                                                                                                                              gcgcGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTccggcaccg                                                                       >  3:291583/1‑98 (MQ=255)                                                                                                                                  ggCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACtcg                  <  7:16562/147‑1 (MQ=255)                                                                                                                                  ggCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACtcg                  >  8:16562/1‑147 (MQ=255)                                                                                                                                      ggACCCTGTCGATGCGCGGCATCGAAAACGCCTCGTACTACCGCCTGATGCCGTACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACtcgcttcgc            <  2:173346/148‑1 (MQ=255)                                                                                                                                        aCCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACACTGGACCTGAGCCACCCCTGCCTGCTGCATGTCGTCACCGCCtcgcttcgct           >  8:250894/1‑148 (MQ=255)                                                                                                                                         cccTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTAc         <  4:214240/149‑1 (MQ=255)                                                                                                                                          gttGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACtcgcttc              >  8:18546/3‑143 (MQ=255)                                                                                                                                           ttGTCGATGCCGCGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTg       >  4:255940/2‑149 (MQ=255)                                                                                                                                           cTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTg       <  7:343975/149‑1 (MQ=255)                                                                                                                                             gTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACt        <  5:109765/146‑1 (MQ=255)                                                                                                                                             gTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACt        >  6:109765/1‑146 (MQ=255)                                                                                                                                                gATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCCTGCTCCAGTTGGTCACCGACTCGCTTCGCTACTGGGccc  >  7:180513/1‑148 (MQ=255)                                                                                                                                                     |                                                                                                                                                CCGCACCCGCGCTACCTGGCCAGCGGCAAGATCGCCGAGTTCAAGGAGATGGTCGCGCACCTGCACGACGCGGGGCTTGAGGTGATCCTGGACGTGGTCTACAACCACACCGCCGAAGGCAACGAGCGCGGCCCGACCCTGTCGATGCGCGGCATCGACAACGCCTCGTACTACCGCCTGATGCCGGACGACAAGCGCTACTACATCAACGACTCCGGCACCGGCAACACCCTGGACCTGAGCCACCCCTGCGTGCTGCAGTTGGTCACCGACTCGCTTCGCTACTGGGCCG  >  NC_002947/4574079‑4574370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑