Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	4,632,595	G→C	11.9%	noncoding (35/76 nt)	PP_t59 ←	tRNA‑Gly

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,632,595	0	G	C	11.9%	211.1 / 14.7	75	noncoding (35/76 nt)	PP_t59	tRNA‑Gly
Reads supporting (aligned to +/- strand):  ref base G (36/30);  new base C (4/5);  total (40/35)
Fisher's exact test for biased strand distribution p-value = 7.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAGG  >  NC_002947/4632448‑4632741                                                                                                                                                    |                                                                                                                                                     gTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGCAGCGGGAAACAAGACTCGAAATCGCTACCCCGACCTTgg                                                                                                                                                      >  8:178836/1‑148 (MQ=255)  tAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGc                                                                                                                                                     >  3:89972/1‑148 (MQ=255)  tAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGc                                                                                                                                                     >  8:299421/1‑148 (MQ=255)   agagGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGc                                                                                                                                                     >  8:114434/1‑147 (MQ=255)     agGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAgg                                                                                                                                                 <  8:257989/149‑1 (MQ=255)       ggCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTc                                                                                                                                               <  7:114434/149‑1 (MQ=255)       ggCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTc                                                                                                                                               >  5:189456/1‑149 (MQ=255)       ggCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTc                                                                                                                                               >  5:265139/1‑149 (MQ=255)         cGGTGTTTTGAGGCAGAATGCATTCGAAGGAAAAAAGGCCCGGTAGGGCCTTTTTTCAACTACTTACAGAGTTCGACTGAAACATGTAGATCTTTATTTGGAGCGGGAAAGGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgg                                                                                                                                             <  8:277138/149‑2 (MQ=25)          ggggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTg                                                                                                                                            <  3:159391/149‑1 (MQ=255)          ggggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggg                                                                                                                                            <  6:265139/149‑3 (MQ=255)            ggTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGct                                                                                                                                          <  8:72201/149‑1 (MQ=255)                     gCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgggg                                                                                                                                           >  2:275988/1‑136 (MQ=255)                     gCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCgggg                                                                                                                                           <  1:275988/139‑4 (MQ=255)                       aGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGa                                                                                                                               <  4:89972/149‑1 (MQ=255)                        gAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCGTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAACCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                              <  2:106031/149‑1 (MQ=255)                             cATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                      >  4:354761/1‑120 (MQ=255)                             cATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTgg                                                                                                                                                      <  3:354761/120‑1 (MQ=255)                              aTTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                              <  4:66842/143‑1 (MQ=255)                              aTTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAg                                                                                                                              >  3:66842/1‑143 (MQ=255)                               ttCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTcc                                                                                                                       >  6:91585/1‑149 (MQ=255)                                tCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                      >  3:230294/1‑149 (MQ=255)                                   gttgaaAAAAGGCCCTTCGGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATACCggcg                                                                                                                   >  1:330121/4‑149 (MQ=21)                                    acgaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgt                                                                                                                  >  3:246108/1‑149 (MQ=255)                                      gaaAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgt‑cg                                                                                                               >  2:214012/1‑149 (MQ=255)                                        aaaaaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGcgt‑cgt                                                                                                              <  2:266225/148‑1 (MQ=255)                                          aaaGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGAt                                                                                                           <  1:214012/149‑1 (MQ=255)                                            aGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATgg                                                                                                         <  1:59470/149‑1 (MQ=255)                                             ggCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATggg                                                                                                        >  7:44930/1‑149 (MQ=255)                                                cccGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGc                                                                                                     >  2:130036/1‑149 (MQ=255)                                                cccGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGc                                                                                                     >  2:182463/1‑149 (MQ=255)                                                      gggCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCt                                                                                               <  8:44930/149‑1 (MQ=255)                                                        gCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAg                                                                                             >  6:91698/1‑149 (MQ=255)                                                         ccTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                      <  5:189151/124‑1 (MQ=255)                                                         ccTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTccc                                                                                                                      >  6:189151/1‑124 (MQ=255)                                                         ccTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGc                                                                                            <  7:341774/149‑1 (MQ=255)                                                                        ttACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGc                                                                             >  7:220017/1‑149 (MQ=255)                                                                        ttACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGc                                                                             <  3:219375/149‑1 (MQ=255)                                                                        ttACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGc                                                                             >  7:214380/1‑149 (MQ=255)                                                                          aCAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCTCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAg                                                                              <  7:178836/146‑1 (MQ=255)                                                                          aCAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggg                                                                                                                                            <  6:348368/85‑3 (MQ=255)                                                                          aCAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGCCAAGGTCggg                                                                                                                                            >  5:348368/1‑83 (MQ=255)                                                                           cAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTc                                                                          <  5:91698/149‑1 (MQ=255)                                                                            aGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTg                                                                                                                                >  8:172250/1‑95 (MQ=255)                                                                              aCTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAAc                                                                       >  4:14089/1‑149 (MQ=255)                                                                                   gACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCt                                                                   <  5:91585/148‑1 (MQ=255)                                                                                   gACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCt                                                                   <  1:130036/148‑1 (MQ=255)                                                                                     cTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGa                                                                <  8:220017/149‑1 (MQ=255)                                                                                     cTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGa                                                                <  8:214380/149‑1 (MQ=255)                                                                                        aaaCCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGAtt                                                              >  1:280472/1‑148 (MQ=255)                                                                                              gTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCaaaaaa                                                       >  2:345959/1‑149 (MQ=255)                                                                                                 gATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACtt                                                    >  4:173134/1‑149 (MQ=255)                                                                                                     tttATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑t                                               >  8:311619/1‑149 (MQ=255)                                                                                                       tATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑tta                                             >  5:130683/1‑148 (MQ=255)                                                                                                       tATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑t                                               <  6:130683/147‑1 (MQ=255)                                                                                                         tttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑ttttt                                           <  4:230294/149‑1 (MQ=255)                                                                                                          ttGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑ttttt                                           <  8:298919/148‑1 (MQ=255)                                                                                                            ggAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑tttttttt                                        >  3:38067/1‑149 (MQ=255)                                                                                                             gAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTc                                       <  4:246108/149‑1 (MQ=255)                                                                                                                   gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTATTTTTTTTTCCGAGc                                  <  3:173134/149‑1 (MQ=255)                                                                                                                   gAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACCTTTA‑TTTTTTTTCCGAGcc                                 >  1:229766/1‑149 (MQ=255)                                                                                                                     aaCGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCtt                               <  7:311619/149‑1 (MQ=255)                                                                                                                        gagaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTcgc                            >  8:291663/1‑149 (MQ=255)                                                                                                                          gaCTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCATGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTcgcg                           >  2:235210/1‑148 (MQ=255)                                                                                                                            cgcGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAg                        <  7:299421/147‑1 (MQ=255)                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTc                                       >  6:160146/1‑133 (MQ=255)                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTc                                       <  5:160146/133‑1 (MQ=255)                                                                                                                             tCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAg                        >  6:207675/1‑148 (MQ=255)                                                                                                                               gAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAg                        <  5:207675/146‑1 (MQ=255)                                                                                                                                     gcgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggc                 >  2:291809/1‑147 (MQ=255)                                                                                                                                      cgACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggccag              >  5:176858/1‑149 (MQ=255)                                                                                                                                       gACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggccagg             <  1:182463/149‑1 (MQ=255)                                                                                                                                         gcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggc            <  6:337656/145‑1 (MQ=255)                                                                                                                                         gcacGACCTTGCCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggc            >  5:337656/4‑148 (MQ=255)                                                                                                                                          cccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcgg          >  5:25706/1‑149 (MQ=255)                                                                                                                                           ccGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGTACGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTgcggccaggcgg          >  1:80965/1‑149 (MQ=255)                                                                                                                                                cTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCAGAGCCTTCGCGCAGGTgctgccaggcggccag      >  6:221516/1‑147 (MQ=255)                                                                                                                                                  tGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAgg  >  6:323518/1‑149 (MQ=255)                                                                                                                                                   ggCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAgg  >  3:136894/1‑148 (MQ=255)                                                                                                                                                    gCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTaa                                                                                   <  8:263210/67‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     GTAGAGGGCGGGGTATTGAGGCAGAATGCATTCGAACGAAAAAAGGCCCCGAAGGGCCTTTTTTCAACGACTTACAGACTTCGACTGAAACCTGTAGATCTTTATTTGGAGCGGGAAACGAGACTCGAACTCGCGACCCCGACCTTGGCAAGGTCGTGCTCTACCAACTGAGCTATTCCCGCGT‑CGTGATGGGTGCCATTCTAGCGATTTCTAAAACAGCGTCAACCCCTTGATTCAAAAAACTTTTA‑TTTTTTTTCCGAGCCTTCGCGCAGGTGCGGCCAGGCGGCCAGCAGG  >  NC_002947/4632448‑4632741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑