Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,635,448 G→A 79.5% T103I (ACC→ATC)  uvrY ← BarA/UvrY two‑componenent system response regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,635,4480GA79.5% 83.3 / 17.8 45T103I (ACC→ATC) uvrYBarA/UvrY two‑componenent system response regulator
Reads supporting (aligned to +/- strand):  ref base G (5/4);  new base A (14/21);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01

CCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACA  >  NC_002947/4635303‑4635594
                                                                                                                                                 |                                                                                                                                                   
ccGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCa                                                                                                                                                  <  6:24996/149‑1 (MQ=255)
ccGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCa                                                                                                                                                  >  4:233734/1‑149 (MQ=255)
          aaCGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCgg                                                                                                                                        >  2:186441/1‑148 (MQ=255)
              cgTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAgcg                                                                                                                                   <  1:180256/149‑1 (MQ=255)
                  gAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCg                                                                                                                                <  8:84317/148‑1 (MQ=255)
                   aaCGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGc                                                                                                                              >  6:137978/1‑149 (MQ=255)
                    aCGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGcc                                                                                                                             >  7:9313/1‑149 (MQ=255)
                              cTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGc                                                                                                                   <  1:186441/149‑1 (MQ=255)
                               tGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCg                                                                                                                  >  7:66922/1‑149 (MQ=255)
                                 cGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCgtg                                                                                                                <  2:93434/149‑1 (MQ=255)
                                       gAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTgggg                                                                                                           >  7:261179/1‑148 (MQ=255)
                                        aaCGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGaa                                                                                                         >  3:150848/1‑149 (MQ=255)
                                           gggttCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGATGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACgg                                                                                                      <  3:241557/146‑1 (MQ=255)
                                           ggTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTACAACAAGCGTGTGGGGAACgg                                                                                                      <  8:9313/149‑1 (MQ=255)
                                           ggTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACgg                                                                                                      <  3:233734/149‑1 (MQ=255)
                                            gTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACggg                                                                                                     >  5:230503/1‑149 (MQ=255)
                                                      cAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTc                                                                                             <  6:230503/147‑1 (MQ=255)
                                                       aaCTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGca                                                                                          <  5:54743/149‑1 (MQ=255)
                                                             tgGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                                        <  7:318050/145‑1 (MQ=255)
                                                             tgGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                                        >  8:318050/1‑145 (MQ=255)
                                                              gggCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGt                                                                                    >  2:258986/1‑148 (MQ=255)
                                                               ggCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGGCCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCa                                                                                  >  7:145792/1‑149 (MQ=255)
                                                                       gCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCAc                                                                           >  7:340080/1‑148 (MQ=255)
                                                                        cGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGTCCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGcc                                                                             <  8:145792/145‑1 (MQ=255)
                                                                        cGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCa                                                                            <  8:66922/146‑1 (MQ=255)
                                                                               atatAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGAt                                                                  <  7:128873/149‑1 (MQ=255)
                                                                                     cgcTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                                        <  6:73973/121‑1 (MQ=255)
                                                                                     cgcTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGcaca                                                                                        >  5:73973/1‑121 (MQ=255)
                                                                                     cgcTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAgg                                                            <  4:150848/149‑1 (MQ=255)
                                                                                              gCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGGATAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTgc                                                    >  1:101368/1‑149 (MQ=255)
                                                                                               cAAAGGCCAGACGAATGGCCTGGCCCAGTTCGTCAAGGCCGGCCACCTTGCGCAGG‑TAGCCGGCAGCGCCGGCCGTCACCACGCGTGGGGGGAACGGGGCTGCCTCGGCCCCCGGCCCCGCCACTACCTTGAGGGCAGGTGGGCTgcg                                                   >  8:241544/1‑148 (MQ=255)
                                                                                               cAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCtt                                                                     <  2:169550/130‑1 (MQ=255)
                                                                                               cAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCtt                                                                     >  1:169550/1‑130 (MQ=255)
                                                                                                    gCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCgcagctg                                             >  8:266312/1‑147 (MQ=255)
                                                                                                     ccAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGt                                            <  5:137978/149‑1 (MQ=255)
                                                                                                        gACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGAGGCTGCGCAGCAGTTTg                                         >  3:225328/1‑149 (MQ=255)
                                                                                                           gAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCgg                                      <  8:261179/149‑1 (MQ=255)
                                                                                                                gCCTGGACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCTGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGc                                 <  7:266312/149‑1 (MQ=255)
                                                                                                                     gACCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCa                            <  2:101368/149‑1 (MQ=255)
                                                                                                                      aCCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGc                                 <  2:217758/143‑1 (MQ=255)
                                                                                                                      aCCATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGc                                 >  1:217758/1‑143 (MQ=255)
                                                                                                                        cATTTCGTCAAGGCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGGGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGgc                         <  4:225328/149‑1 (MQ=255)
                                                                                                                                    gCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCgg             >  4:322523/1‑149 (MQ=255)
                                                                                                                                    gCCTGCACCCTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCgg             <  5:177523/149‑1 (MQ=255)
                                                                                                                                           cccTTGATCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTTCCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCAtttt      <  8:340080/149‑1 (MQ=255)
                                                                                                                                                ggTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTcaca  <  3:322523/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
CCGTTCGGACAACGCGTCGAACGGAGACCCCTGCGGCTGGAACGGTTTCAGCGCCAACTGCTGGGCGATCTGCGGGCTGATATAGCGCTGGCCGGCAAAGGCCAGACGAATGGCCTGGACCATTTCGTCAAGGCCTGCACCCTTGGTCAGG‑TAGCCGGCAGCGCCGGCCTGCAACAAGCGTGTGGGGAACGGGTCTTCCTCGCACACCGTCACCGCCACTACCTTGATGTCAGGGTGGCTGCGCAGCAGTTTGCGGGTGGCCTCCAGGCCGCCGATGCCGGGCATTTTCACA  >  NC_002947/4635303‑4635594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑