Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,676,457 G→T 5.0% T706N (ACC→AAC)  ldcC ← lysine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,676,4570GT 5.0% 264.5 / 3.0 79T706N (ACC→AAC) ldcClysine decarboxylase
Reads supporting (aligned to +/- strand):  ref base G (37/38);  new base T (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.96e-01

CGGCGTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCC  >  NC_002947/4676313‑4676603
                                                                                                                                                |                                                                                                                                                  
cGGCGTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGc                                                                                                                                                <  3:322902/149‑1 (MQ=255)
    gTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCCGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCg                                                                                                                                            >  8:270240/1‑149 (MQ=255)
     tCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCgg                                                                                                                                           <  1:324460/149‑1 (MQ=255)
       gagaTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTg                                                                                                                                         <  8:126315/149‑1 (MQ=255)
        agaTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTg                                                                                                                                         >  1:169262/1‑148 (MQ=255)
          aTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAAc                                                                                                                                      <  7:277764/149‑1 (MQ=255)
           tCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAAc                                                                                                                                      <  2:169262/148‑1 (MQ=255)
               gcgcATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCt                                                                                                                                  >  5:373837/1‑148 (MQ=255)
               gcgcATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCt                                                                                                                                  >  2:290175/1‑148 (MQ=255)
                   aTTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGTTCACCGAGGCGACCCGCTcgatc                                                                                                                             <  3:258578/149‑5 (MQ=255)
                   aTTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATAGAGCGGGTCGCCTCGGTGAACCGCTCAccc                                                                                                                             <  1:31917/149‑1 (MQ=255)
                        gTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCTAGCGGGTCGACTCGGTGAACCGATCACCCGGCAt                                                                                                                        >  8:305334/1‑149 (MQ=255)
                         tGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATg                                                                                                                       <  3:207968/149‑1 (MQ=255)
                          atctcAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGa                                                                                                                      <  1:343336/145‑1 (MQ=255)
                            tGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                     >  3:322391/1‑148 (MQ=255)
                             gCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATc                                                                                                                    >  6:61311/1‑148 (MQ=255)
                              cAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCgg                                                                                                                                           <  5:56444/124‑1 (MQ=255)
                              cAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCgg                                                                                                                                           >  6:56444/1‑124 (MQ=255)
                               aaTCCACCGTGTAGTGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGTTGAACCGCTCACCCGGCATGATCAAc                                                                                                                 >  8:152119/1‑149 (MQ=255)
                               aaTCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGACCCGCTCAGCCGGCATGa                                                                                                                      >  2:343336/1‑144 (MQ=255)
                                aTCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACg                                                                                                                <  6:262972/149‑1 (MQ=255)
                                 tCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACgg                                                                                                               <  1:261984/149‑1 (MQ=255)
                                    aCCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGAt                                                                                                            <  7:18054/149‑1 (MQ=255)
                                        tgtAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCa                                                                                                        <  1:290175/149‑1 (MQ=255)
                                             cGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCgg                                                                                                   <  5:185280/149‑1 (MQ=255)
                                               gCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGTGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCggg                                                                                                  >  5:179222/1‑148 (MQ=255)
                                                 ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGa                                                                                                                                        >  2:311200/1‑108 (MQ=255)
                                                 ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGa                                                                                                                                        <  1:311200/108‑1 (MQ=255)
                                                 ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCaa                                                                                                                  <  2:154795/130‑1 (MQ=255)
                                                 ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCaa                                                                                                                  >  1:154795/1‑130 (MQ=255)
                                                        gTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCAcc                                                                                         <  4:322391/148‑1 (MQ=255)
                                                                   cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                          <  6:229587/120‑1 (MQ=255)
                                                                   cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                          >  5:230148/1‑120 (MQ=255)
                                                                   cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                          >  5:229587/1‑120 (MQ=255)
                                                                   cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                          <  6:230148/120‑1 (MQ=255)
                                                                     tGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGc                                                                                                     >  2:171855/1‑123 (MQ=255)
                                                                     tGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGc                                                                                                     <  1:171855/123‑1 (MQ=255)
                                                                       cacaTCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGTGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCca                                                                         <  6:179222/149‑1 (MQ=255)
                                                                           tCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCACCGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACg                                                                     >  7:314814/1‑149 (MQ=255)
                                                                              gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                     <  6:148596/98‑1 (MQ=255)
                                                                              gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                     >  5:148596/1‑98 (MQ=255)
                                                                              gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGccc                                                                  >  7:225581/1‑149 (MQ=255)
                                                                                     aCCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAccg                                                                               >  2:215674/1‑129 (MQ=255)
                                                                                     aCCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAccg                                                                               <  1:215674/129‑1 (MQ=255)
                                                                                          ggAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTa                                                                                                >  2:327811/1‑107 (MQ=255)
                                                                                          ggAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTa                                                                                                <  1:327811/107‑1 (MQ=255)
                                                                                            aaaGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAcc                                                                                >  4:230177/1‑121 (MQ=255)
                                                                                            aaaGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAcc                                                                                <  3:230177/121‑1 (MQ=255)
                                                                                               gCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCg                                                 <  8:165633/149‑1 (MQ=255)
                                                                                                cccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                <  2:37148/149‑1 (MQ=255)
                                                                                                cccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGAAGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                >  2:338727/1‑149 (MQ=255)
                                                                                                 ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                >  4:199848/1‑148 (MQ=255)
                                                                                                 ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                >  5:191917/1‑148 (MQ=255)
                                                                                                 ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                <  3:199848/148‑1 (MQ=255)
                                                                                                   tGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACgg                                                                                             <  4:294959/101‑1 (MQ=255)
                                                                                                   tGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACgg                                                                                             >  3:294959/1‑101 (MQ=255)
                                                                                                     gTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCg                                           >  5:207524/1‑149 (MQ=255)
                                                                                                       tGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCa                                                                                  <  5:75639/108‑1 (MQ=255)
                                                                                                       tGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCa                                                                                  >  6:75639/1‑108 (MQ=255)
                                                                                                         aaTGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                       <  7:270240/149‑1 (MQ=255)
                                                                                                         aaTGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                       <  6:373837/149‑1 (MQ=255)
                                                                                                           tGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCa                                     <  5:335328/149‑1 (MQ=255)
                                                                                                            gCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCAc                                    >  1:214583/1‑149 (MQ=255)
                                                                                                              tCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACtt                                  >  6:53323/1‑149 (MQ=255)
                                                                                                                gggCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCg                                >  2:99748/1‑149 (MQ=255)
                                                                                                                   cGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCa                             <  5:53323/149‑1 (MQ=255)
                                                                                                                            ggTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATc                    <  7:152119/149‑1 (MQ=255)
                                                                                                                             gTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCt                   >  8:80996/1‑149 (MQ=255)
                                                                                                                             gTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCt                   >  3:106965/1‑149 (MQ=255)
                                                                                                                                 tCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAAc                                                                                                                 >  6:21260/1‑51 (MQ=255)
                                                                                                                                 tCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAAc                                                                                                                 <  5:21260/51‑1 (MQ=255)
                                                                                                                                   aaGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCa              >  4:119623/1‑148 (MQ=255)
                                                                                                                                   aaGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCa              >  4:127768/1‑148 (MQ=255)
                                                                                                                                    ccgggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                       <  6:359646/116‑1 (MQ=38)
                                                                                                                                    ccgggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                       >  5:359646/7‑122 (MQ=38)
                                                                                                                                    aGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATa            <  1:338727/149‑1 (MQ=255)
                                                                                                                                       ggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGc         >  5:337968/4‑149 (MQ=255)
                                                                                                                                       aTCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGc         >  4:127370/1‑149 (MQ=255)
                                                                                                                                          gAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCt       <  3:127370/148‑1 (MQ=255)
                                                                                                                                              gggTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGcc  >  6:8631/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGGCGTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCC  >  NC_002947/4676313‑4676603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑