Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,676,463 T→G 5.0% E704A (GAG→GCG)  ldcC ← lysine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,676,4630TG 5.0% 257.0 / 4.3 80E704A (GAG→GCG) ldcClysine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (38/38);  new base G (2/2);  total (40/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.91e-01

GTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGG  >  NC_002947/4676317‑4676606
                                                                                                                                                  |                                                                                                                                               
gTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCCGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCg                                                                                                                                               >  8:270240/1‑149 (MQ=255)
 tCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCgg                                                                                                                                              <  1:324460/149‑1 (MQ=255)
   gagaTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTg                                                                                                                                            <  8:126315/149‑1 (MQ=255)
    agaTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTg                                                                                                                                            >  1:169262/1‑148 (MQ=255)
      aTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAAc                                                                                                                                         <  7:277764/149‑1 (MQ=255)
       tCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAAc                                                                                                                                         <  2:169262/148‑1 (MQ=255)
           gcgcATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCt                                                                                                                                     >  5:373837/1‑148 (MQ=255)
           gcgcATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCt                                                                                                                                     >  2:290175/1‑148 (MQ=255)
               aTTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGTTCACCGAGGCGACCCGCTcgatc                                                                                                                                <  3:258578/149‑5 (MQ=255)
               aTTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATAGAGCGGGTCGCCTCGGTGAACCGCTCAccc                                                                                                                                <  1:31917/149‑1 (MQ=255)
                    gTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCTAGCGGGTCGACTCGGTGAACCGATCACCCGGCAt                                                                                                                           >  8:305334/1‑149 (MQ=255)
                     tGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATg                                                                                                                          <  3:207968/149‑1 (MQ=255)
                      atctcAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGa                                                                                                                         <  1:343336/145‑1 (MQ=255)
                        tGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                        >  3:322391/1‑148 (MQ=255)
                         gCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATc                                                                                                                       >  6:61311/1‑148 (MQ=255)
                          cAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCgg                                                                                                                                              >  6:56444/1‑124 (MQ=255)
                          cAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCgg                                                                                                                                              <  5:56444/124‑1 (MQ=255)
                           aaTCCACCGTGTAGTGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGTTGAACCGCTCACCCGGCATGATCAAc                                                                                                                    >  8:152119/1‑149 (MQ=255)
                           aaTCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGACCCGCTCAGCCGGCATGa                                                                                                                         >  2:343336/1‑144 (MQ=255)
                            aTCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACg                                                                                                                   <  6:262972/149‑1 (MQ=255)
                             tCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACgg                                                                                                                  <  1:261984/149‑1 (MQ=255)
                                aCCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGAt                                                                                                               <  7:18054/149‑1 (MQ=255)
                                    tgtAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCa                                                                                                           <  1:290175/149‑1 (MQ=255)
                                         cGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCgg                                                                                                      <  5:185280/149‑1 (MQ=255)
                                           gCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGTGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCggg                                                                                                     >  5:179222/1‑148 (MQ=255)
                                             ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGa                                                                                                                                           >  2:311200/1‑108 (MQ=255)
                                             ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGa                                                                                                                                           <  1:311200/108‑1 (MQ=255)
                                             ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCaa                                                                                                                     >  1:154795/1‑130 (MQ=255)
                                             ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCaa                                                                                                                     <  2:154795/130‑1 (MQ=255)
                                                    gTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCAcc                                                                                            <  4:322391/148‑1 (MQ=255)
                                                               cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                             >  5:230148/1‑120 (MQ=255)
                                                               cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                             >  5:229587/1‑120 (MQ=255)
                                                               cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                             <  6:229587/120‑1 (MQ=255)
                                                               cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                             <  6:230148/120‑1 (MQ=255)
                                                                 tGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGc                                                                                                        <  1:171855/123‑1 (MQ=255)
                                                                 tGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGc                                                                                                        >  2:171855/1‑123 (MQ=255)
                                                                   cacaTCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGTGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCca                                                                            <  6:179222/149‑1 (MQ=255)
                                                                       tCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCACCGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACg                                                                        >  7:314814/1‑149 (MQ=255)
                                                                          gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                        >  5:148596/1‑98 (MQ=255)
                                                                          gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                        <  6:148596/98‑1 (MQ=255)
                                                                          gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGccc                                                                     >  7:225581/1‑149 (MQ=255)
                                                                                 aCCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAccg                                                                                  >  2:215674/1‑129 (MQ=255)
                                                                                 aCCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAccg                                                                                  <  1:215674/129‑1 (MQ=255)
                                                                                      ggAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTa                                                                                                   <  1:327811/107‑1 (MQ=255)
                                                                                      ggAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTa                                                                                                   >  2:327811/1‑107 (MQ=255)
                                                                                        aaaGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAcc                                                                                   >  4:230177/1‑121 (MQ=255)
                                                                                        aaaGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAcc                                                                                   <  3:230177/121‑1 (MQ=255)
                                                                                           gCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCg                                                    <  8:165633/149‑1 (MQ=255)
                                                                                            cccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                   <  2:37148/149‑1 (MQ=255)
                                                                                            cccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGAAGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                   >  2:338727/1‑149 (MQ=255)
                                                                                             ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                   <  3:199848/148‑1 (MQ=255)
                                                                                             ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                   >  4:199848/1‑148 (MQ=255)
                                                                                             ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                   >  5:191917/1‑148 (MQ=255)
                                                                                               tGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACgg                                                                                                >  3:294959/1‑101 (MQ=255)
                                                                                               tGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACgg                                                                                                <  4:294959/101‑1 (MQ=255)
                                                                                                 gTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCg                                              >  5:207524/1‑149 (MQ=255)
                                                                                                   tGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCa                                                                                     >  6:75639/1‑108 (MQ=255)
                                                                                                   tGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCa                                                                                     <  5:75639/108‑1 (MQ=255)
                                                                                                     aaTGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                          <  7:270240/149‑1 (MQ=255)
                                                                                                     aaTGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                          <  6:373837/149‑1 (MQ=255)
                                                                                                       tGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCa                                        <  5:335328/149‑1 (MQ=255)
                                                                                                        gCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCAc                                       >  1:214583/1‑149 (MQ=255)
                                                                                                          tCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACtt                                     >  6:53323/1‑149 (MQ=255)
                                                                                                            gggCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCg                                   >  2:99748/1‑149 (MQ=255)
                                                                                                               cGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCa                                <  5:53323/149‑1 (MQ=255)
                                                                                                                        ggTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATc                       <  7:152119/149‑1 (MQ=255)
                                                                                                                         gTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCt                      >  3:106965/1‑149 (MQ=255)
                                                                                                                         gTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCt                      >  8:80996/1‑149 (MQ=255)
                                                                                                                             tCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAAc                                                                                                                    <  5:21260/51‑1 (MQ=255)
                                                                                                                             tCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAAc                                                                                                                    >  6:21260/1‑51 (MQ=255)
                                                                                                                               aaGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCa                 >  4:127768/1‑148 (MQ=255)
                                                                                                                               aaGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCa                 >  4:119623/1‑148 (MQ=255)
                                                                                                                                ccgggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                          <  6:359646/116‑1 (MQ=38)
                                                                                                                                ccgggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                          >  5:359646/7‑122 (MQ=38)
                                                                                                                                aGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATa               <  1:338727/149‑1 (MQ=255)
                                                                                                                                   ggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGc            >  5:337968/4‑149 (MQ=255)
                                                                                                                                   aTCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGc            >  4:127370/1‑149 (MQ=255)
                                                                                                                                      gAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCt          <  3:127370/148‑1 (MQ=255)
                                                                                                                                          gggTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGcc     >  6:8631/1‑149 (MQ=255)
                                                                                                                                             tCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCggg  <  6:191917/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GTCGAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGG  >  NC_002947/4676317‑4676606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑