Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 371,116 C→A 9.5% R199R (CGC→CGA)  dgcA → dimethylglycine dehydrogenase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947371,1160CA 9.5% 191.6 / 8.8 63R199R (CGC→CGA) dgcAdimethylglycine dehydrogenase subunit
Reads supporting (aligned to +/- strand):  ref base C (29/28);  new base A (4/2);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.85e-01

ACTACGCGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTAC  >  NC_002947/370971‑371257
                                                                                                                                                 |                                                                                                                                             
aCTACGCGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATg                                                                                                                                            <  3:11040/149‑1 (MQ=255)
  tACGCGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGa                                                                                                                                           >  8:306886/1‑148 (MQ=255)
  tACGCGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGa                                                                                                                                           >  3:17062/1‑148 (MQ=255)
       gcAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCg                                                                                                                                     >  5:205548/1‑149 (MQ=255)
         aGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCTTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGc                                                                                                                                   >  8:345938/1‑149 (MQ=255)
              gcgcGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGa                                                                                                                              <  7:306886/149‑1 (MQ=255)
               cgcgTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTg                                                                                                                               >  8:23977/1‑147 (MQ=255)
                    gttgcCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTAtt                                                                                                                        <  4:347328/146‑1 (MQ=255)
                        ccAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGa                                                                                                                      >  8:31199/1‑147 (MQ=255)
                         cAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAg                                                                                                                   >  5:183590/1‑149 (MQ=255)
                               gggCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGAATTTCATGCGGCCTTCaaa                                                                                                                            >  5:137250/1‑133 (MQ=255)
                               gggCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGAATTTCATGCGGCCTTCaaa                                                                                                                            <  6:137250/134‑2 (MQ=255)
                                 gcggcCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTAcgc                                                                                                           >  3:240521/1‑149 (MQ=255)
                                      cTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCg                                                                                                       >  2:207409/1‑148 (MQ=255)
                                         gACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGt                                                                                                   >  5:120838/1‑149 (MQ=255)
                                                tGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTgac                                                                                            <  4:240521/149‑1 (MQ=255)
                                                gggAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTgac                                                                                            <  5:157100/148‑1 (MQ=255)
                                                  taaTGGTCGCCAGTGCACCAGCACATTATCTACCTGTGCTGTAGCCAACGGTTTCAGAAGCGTACACATATTTGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTgacga                                                                                          <  1:305407/148‑1 (MQ=255)
                                                  gAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACTAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGaaa                                                                                                                    <  3:114707/123‑1 (MQ=255)
                                                  gAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGaaa                                                                                                                    >  4:114707/1‑123 (MQ=255)
                                                        tCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCt                                                                                     <  6:161128/148‑1 (MQ=255)
                                                        tCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTg                                                                                    >  4:78304/1‑149 (MQ=255)
                                                           gCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGt                                                                                 <  1:207409/149‑1 (MQ=255)
                                                             aGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTgg                                                                               <  7:345938/149‑1 (MQ=255)
                                                               tGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTggg                                                                              >  3:82791/1‑148 (MQ=255)
                                                                   ccAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGc                                                                         <  7:86411/149‑1 (MQ=255)
                                                                      gCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTa                                                                      <  6:183590/149‑1 (MQ=255)
                                                                       cacaTGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTa                                                                                                              >  8:13414/1‑108 (MQ=255)
                                                                       cacaTGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTa                                                                                                              <  7:13414/108‑1 (MQ=255)
                                                                          aTGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTg                                                                  >  5:277681/1‑149 (MQ=255)
                                                                             aTCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCgg                                                               <  7:23977/149‑1 (MQ=255)
                                                                              tCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCAt                                                                           >  5:130863/1‑136 (MQ=255)
                                                                              tCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCAt                                                                           <  6:130863/136‑1 (MQ=255)
                                                                              tCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGt                                                              >  2:236689/1‑149 (MQ=255)
                                                                                gACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGa                                                            >  8:258569/1‑149 (MQ=255)
                                                                                 aCCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                           <  7:31199/149‑1 (MQ=255)
                                                                                 aCCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                           >  4:153714/1‑149 (MQ=255)
                                                                                 aCCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAACCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGAc                                                           <  1:288640/149‑1 (MQ=255)
                                                                                    aGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAg                                                        >  6:320202/1‑149 (MQ=255)
                                                                                     gTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGt                                                       >  6:6247/1‑149 (MQ=255)
                                                                                     gTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGt                                                       <  3:167569/149‑1 (MQ=255)
                                                                                        cTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTc                                                     >  8:157493/1‑148 (MQ=255)
                                                                                         tGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCa                                                   >  5:89170/1‑149 (MQ=255)
                                                                                           gaccccTCGGGTCACCAAGCGTACCTACTCAGGGGGCGCCACATTTGCAGGCGGCATGAAATTCGGCCTTTACGTTTTGAAAGCCGTACGCGCCGTCGTGGGTGACGACTTCTGGGTGGGCATGCGTATCTGCGGTGACGAGTTcccc                                                  <  6:277681/145‑3 (MQ=255)
                                                                                            aGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCAccc                                                <  6:120838/149‑1 (MQ=255)
                                                                                                cACGGGTCAACAATCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTTAAAGCCGTACGAGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCCACCTGAt                                            >  3:209513/1‑149 (MQ=255)
                                                                                                 aCGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATg                                           <  3:78304/149‑1 (MQ=255)
                                                                                                   gggTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGc                                         <  4:82791/149‑1 (MQ=255)
                                                                                                        aacaaGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCa                                     <  6:205548/148‑1 (MQ=255)
                                                                                                        aacaaGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCa                                     <  7:258569/148‑1 (MQ=255)
                                                                                                        aacaaGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTAGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAg                                    >  8:334123/1‑149 (MQ=255)
                                                                                                              cGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGa                              <  3:153714/149‑1 (MQ=255)
                                                                                                                 aCCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCa                                                   >  3:301098/1‑125 (MQ=255)
                                                                                                                 aCCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCa                                                   <  4:301098/125‑1 (MQ=255)
                                                                                                                   cgacgaATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACa                         <  4:17062/149‑1 (MQ=255)
                                                                                                                   agaagaATGGGGCGGAAACTTTGAAGGCCTCATTAAATTCGGCCTTGAAGTATTGAAAGCAGTACGCGCCGAATTGTGTGACGACTTCTGCGTGGGAATGAGTATCTGCGGTGACGAGTTCCACCATGATTGCCTCAGCCACGAGGACa                         >  2:65494/5‑149 (MQ=255)
                                                                                                                            gggCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGa                <  6:89170/149‑1 (MQ=255)
                                                                                                                                  cACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTg          >  7:289839/1‑149 (MQ=255)
                                                                                                                                    tacttcaaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGcc        >  1:79357/7‑149 (MQ=255)
                                                                                                                                    ccTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGcc        >  5:353219/1‑149 (MQ=255)
                                                                                                                                       ttcaaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGAt                                            <  7:221326/107‑1 (MQ=255)
                                                                                                                                       ttcaaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGAt                                            >  8:221326/4‑110 (MQ=255)
                                                                                                                                          aaGGCCGAATTTCATGCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCtactac  >  7:307098/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
ACTACGCGCAGGCTGCGCGTCGCGCCAAAGAGGGCGGCCTGGACGGCGTGGAATTGTCGGCAGTGCACCAGCACATGATCGACCAGTTCTGGAGCCCACGGGTCAACAAGCGTACCGACGAATGGGGCGGCACCTTTGAAGGCCGCATGAAATTCGGCCTTGAAGTATTGAAAGCCGTACGCGCCGAAGTGGGTGACGACTTCTGCGTGGGCATGCGTATCTGCGGTGACGAGTTCCACCCTGATGGCCTCAGCCACGAGGACATGAAGCAGATCGCTGCCTACTAC  >  NC_002947/370971‑371257

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑