Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,676,467 T→C 5.0% T703A (ACC→GCC)  ldcC ← lysine decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,676,4670TC 5.0% 241.6 / 2.1 79T703A (ACC→GCC) ldcClysine decarboxylase
Reads supporting (aligned to +/- strand):  ref base T (37/38);  new base C (2/2);  total (39/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

GAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGC  >  NC_002947/4676320‑4676614
                                                                                                                                                   |                                                                                                                                                   
gagaTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTg                                                                                                                                                    <  8:126315/149‑1 (MQ=255)
 agaTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTg                                                                                                                                                    >  1:169262/1‑148 (MQ=255)
   aTCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAAc                                                                                                                                                 <  7:277764/149‑1 (MQ=255)
    tCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAAc                                                                                                                                                 <  2:169262/148‑1 (MQ=255)
        gcgcATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCt                                                                                                                                             >  5:373837/1‑148 (MQ=255)
        gcgcATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCt                                                                                                                                             >  2:290175/1‑148 (MQ=255)
            aTTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGTTCACCGAGGCGACCCGCTcgatc                                                                                                                                        <  3:258578/149‑5 (MQ=255)
            aTTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATAGAGCGGGTCGCCTCGGTGAACCGCTCAccc                                                                                                                                        <  1:31917/149‑1 (MQ=255)
                 gTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCTAGCGGGTCGACTCGGTGAACCGATCACCCGGCAt                                                                                                                                   >  8:305334/1‑149 (MQ=255)
                  tGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATg                                                                                                                                  <  3:207968/149‑1 (MQ=255)
                   atctcAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGa                                                                                                                                 <  1:343336/145‑1 (MQ=255)
                     tGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                                >  3:322391/1‑148 (MQ=255)
                      gCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATc                                                                                                                               >  6:61311/1‑148 (MQ=255)
                        aaTCCACCGTGTAGTGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGTTGAACCGCTCACCCGGCATGATCAAc                                                                                                                            >  8:152119/1‑149 (MQ=255)
                        aaTCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGACCCGCTCAGCCGGCATGa                                                                                                                                 >  2:343336/1‑144 (MQ=255)
                         aTCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACg                                                                                                                           <  6:262972/149‑1 (MQ=255)
                          tCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACgg                                                                                                                          <  1:261984/149‑1 (MQ=255)
                             aCCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGAt                                                                                                                       <  7:18054/149‑1 (MQ=255)
                                 tgtAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCa                                                                                                                   <  1:290175/149‑1 (MQ=255)
                                      cGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCgg                                                                                                              <  5:185280/149‑1 (MQ=255)
                                        gCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGTGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCggg                                                                                                             >  5:179222/1‑148 (MQ=255)
                                          ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGa                                                                                                                                                   >  2:311200/1‑108 (MQ=255)
                                          ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGa                                                                                                                                                   <  1:311200/108‑1 (MQ=255)
                                          ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCaa                                                                                                                             >  1:154795/1‑130 (MQ=255)
                                          ccccTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCaa                                                                                                                             <  2:154795/130‑1 (MQ=255)
                                                 gTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCAcc                                                                                                    <  4:322391/148‑1 (MQ=255)
                                                            cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                                     <  6:229587/120‑1 (MQ=255)
                                                            cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                                     <  6:230148/120‑1 (MQ=255)
                                                            cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                                     >  5:229587/1‑120 (MQ=255)
                                                            cGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGc                                                                                                                     >  5:230148/1‑120 (MQ=255)
                                                              tGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGc                                                                                                                >  2:171855/1‑123 (MQ=255)
                                                              tGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGc                                                                                                                <  1:171855/123‑1 (MQ=255)
                                                                cacaTCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGTGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCca                                                                                    <  6:179222/149‑1 (MQ=255)
                                                                    tCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCACCGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACg                                                                                >  7:314814/1‑149 (MQ=255)
                                                                       gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                                <  6:148596/98‑1 (MQ=255)
                                                                       gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGAt                                                                                                                                >  5:148596/1‑98 (MQ=255)
                                                                       gCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGccc                                                                             >  7:225581/1‑149 (MQ=255)
                                                                              aCCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAccg                                                                                          <  1:215674/129‑1 (MQ=255)
                                                                              aCCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAccg                                                                                          >  2:215674/1‑129 (MQ=255)
                                                                                   ggAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTa                                                                                                           >  2:327811/1‑107 (MQ=255)
                                                                                   ggAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTa                                                                                                           <  1:327811/107‑1 (MQ=255)
                                                                                     aaaGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAcc                                                                                           >  4:230177/1‑121 (MQ=255)
                                                                                     aaaGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCAcc                                                                                           <  3:230177/121‑1 (MQ=255)
                                                                                        gCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCg                                                            <  8:165633/149‑1 (MQ=255)
                                                                                         cccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                           <  2:37148/149‑1 (MQ=255)
                                                                                         cccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGAAGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                           >  2:338727/1‑149 (MQ=255)
                                                                                          ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                           >  4:199848/1‑148 (MQ=255)
                                                                                          ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                           >  5:191917/1‑148 (MQ=255)
                                                                                          ccTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCgg                                                           <  3:199848/148‑1 (MQ=255)
                                                                                            tGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACgg                                                                                                        <  4:294959/101‑1 (MQ=255)
                                                                                            tGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACgg                                                                                                        >  3:294959/1‑101 (MQ=255)
                                                                                              gTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCg                                                      >  5:207524/1‑149 (MQ=255)
                                                                                                tGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCa                                                                                             <  5:75639/108‑1 (MQ=255)
                                                                                                tGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCa                                                                                             >  6:75639/1‑108 (MQ=255)
                                                                                                  aaTGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                                  <  6:373837/149‑1 (MQ=255)
                                                                                                  aaTGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                                  <  7:270240/149‑1 (MQ=255)
                                                                                                    tGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCa                                                <  5:335328/149‑1 (MQ=255)
                                                                                                     gCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCAc                                               >  1:214583/1‑149 (MQ=255)
                                                                                                       tCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACtt                                             >  6:53323/1‑149 (MQ=255)
                                                                                                         gggCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCg                                           >  2:99748/1‑149 (MQ=255)
                                                                                                            cGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCa                                        <  5:53323/149‑1 (MQ=255)
                                                                                                                     ggTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATc                               <  7:152119/149‑1 (MQ=255)
                                                                                                                      gTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCt                              >  8:80996/1‑149 (MQ=255)
                                                                                                                      gTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCt                              >  3:106965/1‑149 (MQ=255)
                                                                                                                          tCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAAc                                                                                                                            >  6:21260/1‑51 (MQ=255)
                                                                                                                          tCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAAc                                                                                                                            <  5:21260/51‑1 (MQ=255)
                                                                                                                            aaGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCa                         >  4:127768/1‑148 (MQ=255)
                                                                                                                            aaGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCa                         >  4:119623/1‑148 (MQ=255)
                                                                                                                             ccgggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                                  <  6:359646/116‑1 (MQ=38)
                                                                                                                             ccgggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTc                                                  >  5:359646/7‑122 (MQ=38)
                                                                                                                             aGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATa                       <  1:338727/149‑1 (MQ=255)
                                                                                                                                ggtgAGCGGTTCACCGAGGCGACCCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGc                    >  5:337968/4‑149 (MQ=255)
                                                                                                                                aTCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGc                    >  4:127370/1‑149 (MQ=255)
                                                                                                                                   gAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCt                  <  3:127370/148‑1 (MQ=255)
                                                                                                                                       gggTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGcc             >  6:8631/1‑149 (MQ=255)
                                                                                                                                          tCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCggg          <  6:191917/149‑1 (MQ=255)
                                                                                                                                                  gTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCAc                                                        >  6:148766/1‑95 (MQ=255)
                                                                                                                                                  gTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCAc                                                        <  5:148766/95‑1 (MQ=255)
                                                                                                                                                  gTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGc  <  5:8631/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GAGATCACGCGCATTCCGTGATGCAATCCACCGTGTAGCGGCCCCCTTCGTGCTGCAGGCCGTGCACATCGGCGACAAACCCGGGAAAGCCCTGGTTGAATGCTCGGGCGAATGCCAGGTAATCAAGGATCGAGCGGGTCGCCTCGGTGAACCGCTCACCCGGCATGATCAACGGGATGCCAGGCGGGTACGGCACCAGCATCACCGCCGCCACACGCCCCAGCAATGCCTCGATCGGCACCGCCTCCACTTCGCCACGCACCATCTTGTCATAGGCCTGGGCCGGGCTGACGGC  >  NC_002947/4676320‑4676614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑