Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 4,726,619 T→C 7.9% I255V (ATC→GTC)  brnQ ← branched‑chain amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029474,726,6190TC 7.9% 178.0 / 4.3 63I255V (ATC→GTC) brnQbranched‑chain amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base C (3/2);  total (32/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCGCGAGCGAATGGCATTG  >  NC_002947/4726476‑4726755
                                                                                                                                                 |                                                                                                                                        
ggCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGt                                                                                                                                     <  5:86953/149‑1 (MQ=255)
     aGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCtg                                                                                                                                >  7:157595/1‑149 (MQ=255)
             gTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGCCACCAGCCACGACATCGCGGCtgacgccac                                                                                                                         >  3:7489/1‑141 (MQ=38)
                aGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAg                                                                                                                     <  1:241382/149‑1 (MQ=255)
                aGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAg                                                                                                                     >  4:174255/1‑149 (MQ=255)
                  gCCAACCGCGGTCACCAGGCAGTCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGc                                                                                                                   >  2:288512/1‑149 (MQ=255)
                  gCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGc                                                                                                                   >  3:267712/1‑149 (MQ=255)
                    cAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAATGTGAATGATGTACGTAGGCATGCAGCACCCCCGAACCGTTGTTGCCTTCAGCCCCAATTACGTGGATGTTGGCACCCAGGc                                                                                                                   >  1:300992/1‑149 (MQ=255)
                     aaCCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAgg                                                                                                                    <  1:331525/145‑1 (MQ=255)
                     aaCCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAgg                                                                                                                    >  2:331525/1‑145 (MQ=255)
                        cgcgGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCAcc                                                                                                                        <  4:84899/138‑1 (MQ=255)
                        cgcgGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACa                                                                                                             <  5:228198/149‑1 (MQ=255)
                         gcgGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAg                                                                                                            >  7:167031/1‑149 (MQ=255)
                         gcgGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAg                                                                                                            >  7:130086/1‑149 (MQ=255)
                           ggTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGc                                                                                                          <  8:157595/149‑1 (MQ=255)
                           ggTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGc                                                                                                          <  8:126224/149‑1 (MQ=255)
                            gTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCt                                                                                                         <  6:7587/149‑1 (MQ=255)
                            gTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCt                                                                                                         >  8:305224/1‑149 (MQ=255)
                              cACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGa                                                                                                       >  6:231120/1‑149 (MQ=255)
                               aCCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAAc                                                                                                              <  6:66464/141‑1 (MQ=255)
                               aCCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAAc                                                                                                              >  5:66464/1‑141 (MQ=255)
                                ccAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATg                                                                                                     <  1:288512/149‑1 (MQ=255)
                                    gcaggcCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGACGTCGTgg                                                                                                                                   <  3:353375/115‑1 (MQ=255)
                                    gcaggcCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGACGTCGTgg                                                                                                                                   >  4:353375/1‑115 (MQ=255)
                                                 aTCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGccc                                                                                    <  8:167031/149‑1 (MQ=255)
                                                    aGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGa                                                                                  >  7:339009/1‑148 (MQ=255)
                                                     gCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCACGACATCGCGGCtga                                                                                                                               <  6:227167/102‑2 (MQ=255)
                                                     gCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCACGACATCGCGGCtga                                                                                                                               >  5:227167/1‑101 (MQ=255)
                                                           ccAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGaa                                                                                                               <  1:134794/112‑1 (MQ=255)
                                                           ccAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGaa                                                                                                               >  2:134794/1‑112 (MQ=255)
                                                             aGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                                                        <  3:325141/149‑1 (MQ=255)
                                                             aGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                                                        <  3:174255/149‑1 (MQ=255)
                                                              ggAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATc                                                                       <  8:289666/149‑1 (MQ=255)
                                                               gAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAgg                                                                                            <  8:237986/127‑1 (MQ=255)
                                                               gAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                                                        >  5:148773/1‑147 (MQ=255)
                                                               gAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                                                        >  7:229583/1‑147 (MQ=255)
                                                               gAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGAt                                                                        >  7:106221/1‑147 (MQ=255)
                                                               gAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCCCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAgg                                                                                            >  7:237986/1‑127 (MQ=255)
                                                                 aGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAgg                                                                    >  2:167305/1‑149 (MQ=255)
                                                                    cGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCg                                                                 >  3:238396/1‑149 (MQ=255)
                                                                         cccAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCgat                                                            >  5:271585/1‑149 (MQ=255)
                                                                          ccAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCgat                                                            >  1:367171/1‑148 (MQ=255)
                                                                          ccAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCgat                                                            >  5:116766/1‑148 (MQ=255)
                                                                           cAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCgatga                                                          >  6:41346/1‑149 (MQ=255)
                                                                           cAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCgatga                                                          >  5:206056/1‑149 (MQ=255)
                                                                             gggAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGatgatg                                                        <  6:148773/149‑1 (MQ=255)
                                                                              ggAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATgg                                                       <  4:267712/149‑1 (MQ=255)
                                                                                     aaGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCg                                                >  2:93198/1‑149 (MQ=255)
                                                                                      aGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCgg                                               <  7:305224/149‑1 (MQ=255)
                                                                                             tGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTaa                                           <  2:300992/148‑1 (MQ=255)
                                                                                                      tAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAg                                       >  7:44049/1‑143 (MQ=255)
                                                                                                      tAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAg                                       <  8:44049/143‑1 (MQ=255)
                                                                                                            tGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGAGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTc                           <  4:331279/149‑1 (MQ=255)
                                                                                                               agcaCCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGa                         >  2:8606/1‑148 (MQ=255)
                                                                                                                      ccgcACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTc                           >  6:268955/1‑139 (MQ=255)
                                                                                                                      ccgcACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTc                           <  5:268955/139‑1 (MQ=255)
                                                                                                                       cgcACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACcgcgcg                <  8:339009/149‑1 (MQ=255)
                                                                                                                        gcACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCgcga               <  2:367171/149‑1 (MQ=255)
                                                                                                                              ttGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCGCGAGCGAAt         <  6:116766/149‑1 (MQ=255)
                                                                                                                                 gtggCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCGCGAGCGAATGGc      <  1:167305/149‑1 (MQ=255)
                                                                                                                                     cGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCGCGAGCGAATGGCATTg  <  1:93198/149‑1 (MQ=255)
                                                                                                                                            cgcgATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCgcgag              <  4:236093/130‑1 (MQ=255)
                                                                                                                                            cgcgATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCgcgag              >  3:236093/1‑130 (MQ=255)
                                                                                                                                                 |                                                                                                                                        
GGCGCAGGCACAGGTCAGGCCAACCGCGGTCACCAGGCAGGCCAGCGCGATCAGCACGGCCAGGAAGCCGCTGCCCAGGGAGCCGAAGGT‑‑ATGCTGTACGTAGGCATGCAGCACCGCCGCACCGTTGGTGGCGTCAGCCGCGATGTCGTGGCTGGTGGCACCCAGGCGGAACAGGCTGATGTAGACCAGGGCCAGCCCGACACCGGCGATCAGGCCGGCGATGATGGCGTAGCGGGTAATCAGCCTCGGCGACTCGACACCGCGCGAGCGAATGGCATTG  >  NC_002947/4726476‑4726755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑