| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 91.7% | 69.8 / 0.9 | 24 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (2/0); new base C (11/11); total (13/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTC > NC_002947/1932499‑1932773 | ggttCGAAGAGTAGCTGGACATTCGACTGCGGCATTGAAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCGTTGGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGaa < 2:99733/146‑1 (MQ=38) cgcaTCTGGACATTCGACCGCGCCATTGCAGGCCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCCCGGGCAAGCCCGCTCCTACAAAGCATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCaa < 6:23910/145‑1 (MQ=38) cTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTGCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCCAGGCAg > 8:89683/1‑148 (MQ=255) tGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGCTATGCGTCCCCGGTAGGAG‑CGGGCTT‑GCCCGCGCAGGGCTGCCAGGCAGc > 3:6651/1‑148 (MQ=38) ggACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGcc > 4:9999/1‑148 (MQ=255) aTTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCaa > 3:36837/1‑148 (MQ=255) cGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAACGAAATGCCGCCCCTGTTGGGG‑CGGGCTTGG‑CCCCGCACGGGTGCCAGGGAGCACCAAAATggagg > 4:262/1‑143 (MQ=25) aTTGCAGGTCTCACTAAAATAGCAGTAACCGCGGGGGCCGCTTTGCGGCCCTGCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑GGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTAcg < 8:4100/148‑1 (MQ=37) tcACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCGCGCCTAAAAAGGATATTCGGCCCCAGTTGGAGACGGCGTT‑C‑CCGCGCAGGGCAGCAAGGCAACCCCAAATTGTCGCAGACGCCGATCAGc > 6:218/1‑147 (MQ=255) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGGAGGAG‑CGGGCTT‑GCCCGCGCAGGGCTGCAAGGCCGCCCCAAATTGTCGACTACGCGGCTCAGCGAt > 6:120069/1‑148 (MQ=37) cGAAAATAGCAGTAAACGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGCATAGCCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc < 4:107219/148‑1 (MQ=37) aTAGCAGTAACCGCTGGGGCCGCTTGGCGGCCCTTCGCGGGCAACCCCGCTCCTACAAATGATAGGCGGCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCa < 8:120979/148‑1 (MQ=38) tAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCGCTGATCCgcgta < 4:36837/148‑5 (MQ=37) tttGGGGCCGTTCGCGGGCAAGCCCGCGCCTACAACGTATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATc < 4:6651/148‑1 (MQ=38) ggCCGTGCGCGGGCAAGCCCGCTCCTACAACGGATATGCGGCCCCTGTAGGAG‑GGGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagca < 5:38566/148‑1 (MQ=255) cccTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGagcagc > 6:31723/1‑148 (MQ=255) aaGCCCGCTCCTACAAAGGATATGGGTCCCCTGTAGGAG‑CGGGCTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt < 3:127769/148‑1 (MQ=255) aaGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGGTCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt > 6:25311/1‑148 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGc > 5:28407/1‑148 (MQ=255) cggatcggCGGCCCCGGTAGGCG‑GGGGGTG‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGcac < 3:3586/141‑1 (MQ=255) atatGCGTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCAcatc < 4:36870/148‑1 (MQ=255) gTCCCCTGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGtt > 2:78392/1‑148 (MQ=255) tGTAGGAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGAt > 8:91512/1‑148 (MQ=255) gAG‑CGGGCTT‑GCCCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc > 2:10720/1‑147 (MQ=255) | TCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAG‑CGGGCTT‑G‑CCGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTC > NC_002947/1932499‑1932773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |