Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947195,6340CT30.6% 40.0 / 24.2 36T380T (ACC→ACT) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (13/12);  new base T (8/3);  total (21/15)
Fisher's exact test for biased strand distribution p-value = 2.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.57e-01
Rejected as consensus: Frequency below/above cutoff threshold.

ATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195489‑195778
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aTTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGAcaccacca                                                                                                                                                 <  8:31650/147‑1 (MQ=39)
    ccAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACcgcg                                                                                                                                            >  6:126070/1‑148 (MQ=39)
                             aacaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCa                                                                                                                   >  3:44550/1‑148 (MQ=37)
                              acaacGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCAc                                                                                                                  >  7:70363/1‑148 (MQ=17)
                                   ggTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAg                                                                                                             <  1:137472/148‑1 (MQ=37)
                                         aCCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGgcggcg                                                                                                       >  8:88503/1‑148 (MQ=37)
                                           cGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg                                                                                                     <  5:126070/148‑1 (MQ=38)
                                           cGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTg                                                                                                     <  2:14993/148‑1 (MQ=38)
                                             tCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt                                                                                                   <  1:116471/148‑1 (MQ=39)
                                             tCAGCGTCACCATCGAGAACGCCACAGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGAt                                                                                                   <  3:2081/148‑1 (MQ=17)
                                                gCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCa                                                                                                 <  5:64771/147‑1 (MQ=38)
                                                             gaACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGAcc                                                                                   <  2:9661/148‑1 (MQ=39)
                                                                 gCCACCGGCGGTAATTTCGAGCAACTGACCCCGACTCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGa                                                                               <  4:23156/148‑1 (MQ=255)
                                                                   cACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCCCCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAg                                                                              >  5:136882/1‑147 (MQ=255)
                                                                      aggcggTAATTTCGAGCAACTGACCCCGAATCCGACCAATGCTCAGACAACGATCAAAGAATCTGTCGACACCACTACCGCGACACTGACGGCGATCCCTTCGGTAACCTAAGTCGGCGTGATAACTTAAACCGTTACCATGAGCaa                                                                           >  2:98850/2‑147 (MQ=11)
                                                                        gcggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGCCCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTCACACCGTGACCCTGAGCAAccc                                                                        >  6:111723/1‑148 (MQ=255)
                                                                          ggTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGGGCAACCCTg                                                                      >  1:124572/1‑148 (MQ=21)
                                                                            tAATTTCGAGCGACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGcc                                                                    >  3:51105/1‑148 (MQ=255)
                                                                            tAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGc                                                                     >  8:115277/1‑147 (MQ=17)
                                                                            tAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGATGGCGAGCCCGTCGTTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGc                                                                     >  1:134788/1‑147 (MQ=255)
                                                                             aaTTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCAc                                                                                                <  4:18356/119‑1 (MQ=17)
                                                                                  cGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGAc                                                               <  7:88503/147‑1 (MQ=39)
                                                                                   gAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACCCCGTGACCCTGAGCAACCCTGCCCAGACGc                                                             >  8:89610/1‑148 (MQ=21)
                                                                                     gCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCg                                                           <  3:117317/148‑1 (MQ=39)
                                                                                         cTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGtga                                                       >  4:13136/1‑146 (MQ=2)
                                                                                             ccccGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGATGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGt                                                   <  3:41259/148‑1 (MQ=12)
                                                                                                       gACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTc                                          >  2:12700/1‑147 (MQ=255)
                                                                                                        aCCCCGGCTCATACCACGATCAAACACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCAACGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCAGTCCa                                        >  8:82599/1‑148 (MQ=14)
                                                                                                        aCCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGa                                                                                                                               >  5:88955/1‑61 (MQ=35)
                                                                                                         ccccGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAg                                                                                                                              <  6:88955/61‑1 (MQ=33)
                                                                                                                   gACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACg                             <  6:137958/148‑1 (MQ=38)
                                                                                                                   gACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACTTACACCGt                                                                                       >  1:34470/1‑90 (MQ=18)
                                                                                                                    aCCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAACGCGGCGTGATCACTTACACCGTGCCCCTGAGCAACCCTCCCCAGACGCCGGTACACGTGCACATGTCCAACGGCCATACGa                            >  7:121931/1‑148 (MQ=14)
                                                                                                                     ccACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGa                            >  3:65870/1‑147 (MQ=38)
                                                                                                                         gATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTAcc                       >  5:89528/1‑148 (MQ=255)
                                                                                                                                   tCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCgg             <  4:44550/148‑1 (MQ=39)
                                                                                                                                     ggTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTTATCACTTACACCGTGACCCTGAGCACCCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGCCCAAAAGATTACCGTTGAAGCggg            >  2:41218/1‑147 (MQ=14)
                                                                                                                                              cactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGCAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  >  1:104408/1‑148 (MQ=255)
                                                                                                                                              cactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  <  7:89610/148‑1 (MQ=255)
                                                                                                                                              cactaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  >  5:99819/1‑148 (MQ=255)
                                                                                                                                              caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  <  5:56150/148‑1 (MQ=39)
                                                                                                                                              caccaccGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAg  >  4:62554/1‑148 (MQ=39)
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ATTTCCAGACCCCGGCCAATGACGTCTACAACAACGGTTCGACCGTCAGCGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTAACCGTGAACCTGTCCAACGGCCAAACGATTACCGTTGAAGCGGGTAAAACCCAG  >  NC_002947/195489‑195778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑