Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947196,3060TC20.6% 61.4 / 12.5 36N604N (AAT→AAC) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (14/13);  new base C (4/3);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTC  >  NC_002947/196162‑196441
                                                                                                                                                |                                                                                                                                       
cGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCt                                                                                                                                      <  3:63013/148‑1 (MQ=39)
               aCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTg                                                                                                                       <  8:125932/148‑1 (MQ=35)
                       cGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc                                                                                                               >  7:14742/1‑148 (MQ=39)
                            ccGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt                                                                                                          >  4:9415/1‑148 (MQ=39)
                                   cGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACgg                                                                                                   >  6:130505/1‑148 (MQ=39)
                                         aGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCaaa                                                                                              >  8:79846/1‑147 (MQ=39)
                                          gACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAAACCTGCCCAGACGCCGGTAACAGTGACCCTGTCCAACGGCCAAAcg                                                                                            >  4:36563/1‑147 (MQ=9)
                                             cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                         >  5:63682/1‑148 (MQ=39)
                                             cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGGCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                         <  8:81832/148‑1 (MQ=255)
                                             cACGATCAACGACTCGGTCGACACCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                         >  4:119879/1‑148 (MQ=21)
                                                                     cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg                                                                 <  3:9415/148‑1 (MQ=17)
                                                                     cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAg                                                                 >  5:132887/1‑148 (MQ=37)
                                                                         aCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCa                                                             <  4:43727/148‑1 (MQ=12)
                                                                            gcgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATcc                                                                                                                                       >  5:64343/1‑71 (MQ=34)
                                                                             cgACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCt                                                                                                                                      <  6:64343/71‑1 (MQ=34)
                                                                                 ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAt                                                     >  8:84488/1‑148 (MQ=34)
                                                                                 ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAt                                                     <  2:146660/148‑1 (MQ=34)
                                                                                      cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                                <  6:63682/148‑1 (MQ=34)
                                                                                      cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                                <  7:84488/148‑1 (MQ=34)
                                                                                      cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCa                                                <  7:115277/148‑1 (MQ=255)
                                                                                             cccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCg                                         >  6:66905/1‑148 (MQ=34)
                                                                                                      cACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc                                <  2:78235/148‑1 (MQ=255)
                                                                                                        ccGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCg                                                                                                                          <  5:138932/56‑1 (MQ=35)
                                                                                                         cGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATcc                                                                                                                                       >  1:60802/1‑42 (MQ=38)
                                                                                                          gAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCt                                                                                                                                      <  2:60802/42‑1 (MQ=38)
                                                                                                                       tCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGAc                                                                                                                     >  6:78827/1‑46 (MQ=38)
                                                                                                                       tCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGGTc               >  8:100209/1‑148 (MQ=21)
                                                                                                                        cACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa                                                                                                                    <  5:78827/46‑1 (MQ=38)
                                                                                                                         aCTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa             >  7:20257/1‑148 (MQ=255)
                                                                                                                           ttACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                           >  3:112618/1‑68 (MQ=14)
                                                                                                                           ttACACCGTGACCCTGAGCAAACCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                           >  3:114550/1‑68 (MQ=14)
                                                                                                                               acCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa       <  8:14742/148‑1 (MQ=34)
                                                                                                                               acCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa       >  8:52626/1‑148 (MQ=255)
                                                                                                                                    gACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTc  >  6:138172/1‑148 (MQ=11)
                                                                                                                                       ccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa             >  6:90002/1‑134 (MQ=32)
                                                                                                                                        cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAc            <  5:90002/134‑1 (MQ=32)
                                                                                                                                                |                                                                                                                                       
CGGTAATTTCGAGCAACTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTC  >  NC_002947/196162‑196441

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑