Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947196,3510CG22.9% 55.6 / 17.8 35T619T (ACC→ACG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base C (17/10);  new base G (4/4);  total (21/14)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGAC  >  NC_002947/196207‑196494
                                                                                                                                                |                                                                                                                                               
cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                                                                              >  5:63682/1‑148 (MQ=39)
cACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGGCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                                                                              <  8:81832/148‑1 (MQ=255)
cACGATCAACGACTCGGTCGACACCACAACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACATACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCAtt                                                                                                                                              >  4:119879/1‑148 (MQ=21)
                        cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAGACCCAg                                                                                                                      <  3:9415/148‑1 (MQ=17)
                        cacTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAg                                                                                                                      >  5:132887/1‑148 (MQ=37)
                            aCCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCa                                                                                                                  <  4:43727/148‑1 (MQ=12)
                                    ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAt                                                                                                          >  8:84488/1‑148 (MQ=34)
                                    ccTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGAt                                                                                                          <  2:146660/148‑1 (MQ=34)
                                         cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                                                                                     <  7:84488/148‑1 (MQ=34)
                                         cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCa                                                                                                     <  6:63682/148‑1 (MQ=34)
                                         cGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCa                                                                                                     <  7:115277/148‑1 (MQ=255)
                                                cccGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCg                                                                                              >  6:66905/1‑148 (MQ=34)
                                                         cACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGAc                                                                                     <  2:78235/148‑1 (MQ=255)
                                                                          tCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGGTc                                                                    >  8:100209/1‑148 (MQ=21)
                                                                            aCTTACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa                                                                  >  7:20257/1‑148 (MQ=255)
                                                                              ttACACCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                >  3:112618/1‑68 (MQ=14)
                                                                              ttACACCGTGACCCTGAGCAAACCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCa                                                                                                                                                >  3:114550/1‑68 (MQ=14)
                                                                                  acCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa                                                            <  8:14742/148‑1 (MQ=34)
                                                                                  acCGTGACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCa                                                            >  8:52626/1‑148 (MQ=255)
                                                                                       gACCCTGAGCAACCCTGCCCAGACGCCGGTGACCGTGACCCTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGCGTc                                                       >  6:138172/1‑148 (MQ=11)
                                                                                          ccTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGa                                                                  >  6:90002/1‑134 (MQ=32)
                                                                                           cTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGAc                                                                 <  5:90002/134‑1 (MQ=32)
                                                                                                     cTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACg                                          >  1:130098/1‑147 (MQ=37)
                                                                                                        cccAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCAc                                      >  5:69633/1‑148 (MQ=37)
                                                                                                                  ggTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGCCCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAAt                            >  6:6021/1‑148 (MQ=255)
                                                                                                                        aGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGCCCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCCCCGGCGGTAATTTCGa                       >  3:7438/1‑147 (MQ=255)
                                                                                                                             cccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAAc                  <  6:132887/147‑1 (MQ=37)
                                                                                                                              ccTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCaaa                                                                                                                            >  7:36906/1‑40 (MQ=33)
                                                                                                                               cTGTCCAACGGCCAAACGATTACCGTTGAAGCCGGCaaaa                                                                                                                           <  8:36906/40‑1 (MQ=255)
                                                                                                                               cTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGt                                                                                   <  6:68602/80‑1 (MQ=21)
                                                                                                                                     aaCGGCCAAACGATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATGACGTCTa                                                                                >  5:103899/1‑77 (MQ=255)
                                                                                                                                       cGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAAt       >  4:111449/1‑148 (MQ=38)
                                                                                                                                         gCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGACTTCCAGACCCCGGCGAATg                                                                                       <  1:32399/66‑1 (MQ=255)
                                                                                                                                            aaaCCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACACCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGAc  >  5:136206/1‑148 (MQ=255)
                                                                                                                                            aaaCCAATACCGTTGAAGCCGGCAAAACAGAGGGCAGCGTCGATTTCCAGACCCCCGCGAATGACGTCTAAAACAACGGTTCGACTCTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATATAGAGCAACTGAACCCGAATCCGAc  >  1:2527/1‑148 (MQ=14)
                                                                                                                                                |                                                                                                                                               
CACGATCAACGACTCGGTCGACACCACTACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACTTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGTCCAACGGCCAAACCATTACCGTTGAAGCCGGCAAAACCCAGGGCAGCGTCGATTTCCAGACCCCGGCGAATGACGTCTACAACAACGGTTCGACTGTCAGTGTCACCATCGAGAACGCCACCGGCGGTAATTTCGAGCAACTGACCCCGAATCCGAC  >  NC_002947/196207‑196494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑