Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947524,3490GC23.1% 49.0 / 12.5 26intergenic (+216/‑600)tyrS/PP_16SCtyrosine‑‑tRNA ligase/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (10/10);  new base C (5/1);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.33e-01
Rejected as consensus: Frequency below/above cutoff threshold.

TGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCA  >  NC_002947/524212‑524488
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tgttgtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCAGCGCCTATGAGACCGAGCGCCGCCCGCGCGgcgc                                                                                                                                   >  3:31647/1‑148 (MQ=255)
    gtAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCATGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCgg                                                                                                                               >  7:116195/1‑148 (MQ=255)
             gggggCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCAGCGCCTATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAgg                                                                                                                      <  4:31647/148‑1 (MQ=25)
                           gagGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc                                                                                                         <  2:98914/147‑1 (MQ=255)
                           gagGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc                                                                                                         <  3:2646/147‑1 (MQ=255)
                               gtgtTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGAGCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGc                                                                                                    <  7:13109/148‑1 (MQ=255)
                                                        acaACCTTTGCTGCGCATGTGAGTTCGAGCGCGGCCCGCGTCCGGCTTGAGACATTCACCGCATATTAAAACTAGCGCCGGGGGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGt                                                                           <  2:125942/148‑1 (MQ=21)
                                                         caACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGGCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTc                                                                          <  4:114238/148‑1 (MQ=255)
                                                               ttGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCAGGATTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGGCTCATAGGCGCTGAACCTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCGTTTcc                                                                    >  8:50667/1‑148 (MQ=37)
                                                                               aTCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGt                                                                                                          >  6:126332/1‑94 (MQ=255)
                                                                                tCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTc                                                                                                         <  5:126332/94‑1 (MQ=255)
                                                                                          cccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCCTAGGCGCTGCAACTGTCGTGGGGAACACTTGGCCGCCTCGATACCGGCTTTTCCCCTTATATTTCACTTTCTTGAAAtt                                           >  6:13568/1‑146 (MQ=21)
                                                                                          cccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAACCTGTCGTGGCGAACACTTGGCCGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAAtt                                           >  1:65076/1‑146 (MQ=255)
                                                                                           ccgcGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTaaa                                        <  3:54893/148‑1 (MQ=255)
                                                                                             gcgTCCGTCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGTGCGGCGCTCGGTCTCATAGTCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGTTACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg                                      <  5:64910/148‑1 (MQ=255)
                                                                                             gcgTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAgg                                      <  1:20529/148‑1 (MQ=255)
                                                                                                         agaCATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCctct                          >  1:118785/1‑148 (MQ=255)
                                                                                                               tCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATc                    <  2:98091/148‑1 (MQ=255)
                                                                                                                       tATGAGACCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTtata            >  4:132243/1‑148 (MQ=255)
                                                                                                                         tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAATGTTGACGCCCTCTCGAATCACCTTATAAt          >  7:82051/1‑148 (MQ=255)
                                                                                                                         tGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTGCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAAt          >  7:84040/1‑148 (MQ=255)
                                                                                                                            gaCCGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATgcg       >  5:35408/2‑148 (MQ=255)
                                                                                                                               cGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGccc    >  3:73920/1‑148 (MQ=255)
                                                                                                                               cGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGGTGAAACTGTCGTGGCGACCCCTGGGCCGCCTCGAGACGGTCTTTGCCCCGTATATTTCACTTTCTTGAAATTACCGGTTGACGCCCTCTCGCATCCCCTTATAATGCGccc    >  7:91809/1‑148 (MQ=11)
                                                                                                                               cGAGCGCCGCCCGCGCGGCGCTCGGTTTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGAGcc     >  2:107641/1‑147 (MQ=255)
                                                                                                                                 aGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCa  >  8:111950/1‑148 (MQ=255)
                                                                                                                                      cgcgcgGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAAt                                            >  3:71133/1‑101 (MQ=255)
                                                                                                                                       gcgcgGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAAtt                                           <  4:71133/101‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TGTTGTAGGGGGAGGGGGCTGTTTTGAGAGGGTGTTGGCGGTGAGATGTGTCCGCCACAACCCTTGCAGCGCCTGTGAGATCGAGCGCCGCCCGCGTCCGGCTTGAGACATTCACCGCCTATGAAACCGAGCGCCGCGCGGGCGGCGCTCGGTCTCATAGGCGCTGAAACTGTCGTGGCGAACACTTGGCAGCCTCGATACGGTCTTTTCCCCTTATATTTCACTTTCTTGAAATTAAAGGTTGACGCCCTCTCGAATCCCCTTATAATGCGCCCCA  >  NC_002947/524212‑524488

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑