Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,715,145	C→T	R126C (CGC→TGC)	PP_4173 →	two‑component system sensor histidine kinase/response regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,715,145	0	C	T	100.0%	56.7 / NA	18	R126C (CGC→TGC)	PP_4173	two‑component system sensor histidine kinase/response regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/5);  total (13/5)

CCTCAGCATCCCGCACGTGCGCTTTCTGGAAGTGCAGGACAGCCGCAACCACATCCTGGTGTACGTCGAGCAGTCCGACGAAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCCGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAATCGTGATCAAGGCCGGAA  >  NC_002947/4715003‑4715283                                                                                                                                               |                                                                                                                                           ccTCAGCATCCCGCACGTGCGCTTTCTGGAAGTGCAGGACAGCCGCAACCACATCCTGGTGTACGTCGAGCAGTCCGACGAAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCt                                                                                                                                        >  8:20729/1‑147 (MQ=255)       cATCCCGCACGTGCGCTTTCTGGAAGTGCAGGACAGCCGCAACCACATCCTGGTGTACGTCGAGCAGTCCGACGAAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACaa                                                                                                                                  >  2:88739/1‑147 (MQ=255)          cccGCACGTGCGCTTTCTGGAAGTGCAGGACAGCCGAAACCACATACACGTGTACGTCGAGCAGTCCGACGAAAGTGCTAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGAc                                                                                                                              >  2:25562/1‑148 (MQ=255)                                                 ccACATCCTGGTGTACGTCGAGCAGTCCGACGAAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCgg                                                                                        >  3:54844/1‑147 (MQ=255)                                                               aCGTCGAGCAGTCCGACGAAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTggg                                                                         >  3:77083/1‑148 (MQ=255)                                                                                gaAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCa                                                        >  4:38081/1‑148 (MQ=255)                                                                                       ccAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGa                                                 >  1:32730/1‑148 (MQ=255)                                                                                                cgcAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCtt                                        <  1:88739/148‑1 (MQ=255)                                                                                                  cAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTtca                                      <  7:20729/148‑1 (MQ=255)                                                                                                           gAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAgcg                               >  7:64805/1‑146 (MQ=255)                                                                                                                    cAGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGAGGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGaaa                    >  5:31/1‑148 (MQ=255)                                                                                                                     aGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGaaa                    <  6:18193/147‑1 (MQ=255)                                                                                                                     aGGCGCCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAAt                   >  7:34425/1‑148 (MQ=255)                                                                                                                         gcCCATTCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAATCGTg               <  8:2467/148‑1 (MQ=255)                                                                                                                               tCGCCTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAATCGTGATCAAg         <  1:104984/148‑1 (MQ=255)                                                                                                                                  ccTGCAGCAAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAATCGTGATCAAGGcc      >  1:113739/1‑148 (MQ=255)                                                                                                                                      cagcaAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGCCGCCTTCAGTCAGCGCCAGCAGGAAATCGTGATCAAGGCCGGaa  >  2:62208/1‑148 (MQ=255)                                                                                                                                      cagcaAATCTGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAATCGTGATCAAGGCCGGaa  >  2:64243/1‑148 (MQ=255)                                                                                                                                               |                                                                                                                                           CCTCAGCATCCCGCACGTGCGCTTTCTGGAAGTGCAGGACAGCCGCAACCACATCCTGGTGTACGTCGAGCAGTCCGACGAAAGTGCCAACCGCGCGCAACAGGTCGAGGTGTTCCAGGCGCCCATTCGCCTGCAGCAAATCCGCCTGGACAACGACTTCCTGCAACAGGGCAAAGCCACCAGCCCGGCCATCGGTGACGACTACCTGGGCCGCGTGATCGTCGGCATGTCCGATGACGCCTTCAGTCAGCGCCAGCAGGAAATCGTGATCAAGGCCGGAA  >  NC_002947/4715003‑4715283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑