Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,964,741	A→C	I206S (ATC→AGC)	fleQ ←	transcriptional regulator FleQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,964,741	0	A	C	100.0%	82.7 / NA	25	I206S (ATC→AGC)	fleQ	transcriptional regulator FleQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/11);  total (14/11)

CAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCGTCGGTATCCGCCACTTGCTGCATCATCT  >  NC_002947/4964602‑4964885                                                                                                                                            |                                                                                                                                                 cAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGCACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCca                                                                                                                                          >  2:119272/1‑148 (MQ=255)           tCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCg                                                                                                                                >  5:46610/1‑148 (MQ=255)             gCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGc                                                                                                                              >  1:60178/1‑148 (MQ=255)                     cgtcgAGGAACAGGGTACCGCAGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACg                                                                                                                       <  4:75496/147‑1 (MQ=255)                                     aCCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTg                                                                                                      <  8:2168/148‑1 (MQ=255)                                               gCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGt                                                                                            >  2:60763/1‑148 (MQ=255)                                               gCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGt                                                                                            >  2:62846/1‑148 (MQ=255)                                               gCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGt                                                                                            >  1:121883/1‑148 (MQ=255)                                                 cAGTTCGAAACGCCCGTCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGc                                                                                          <  3:19529/148‑1 (MQ=255)                                                  aGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCa                                                                                         <  1:65312/148‑1 (MQ=255)                                                                           tGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTtgcc                                                                <  7:8970/148‑1 (MQ=255)                                                                                 ccccGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTtgccggtgcc                                                          <  8:74594/148‑1 (MQ=255)                                                                                 ccccGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTtgccggtgcc                                                          <  2:6204/148‑1 (MQ=255)                                                                                 ccccGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTtgccggtgcc                                                          >  5:15526/1‑148 (MQ=255)                                                                                  cccGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTCCCACTTCCTtgccggtgccg                                                         >  1:110591/1‑148 (MQ=255)                                                                                    cGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGa                                                       <  1:39991/148‑1 (MQ=255)                                                                                     ggTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGAc                                                      <  4:18504/148‑1 (MQ=255)                                                                                                             gAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCg                              >  4:81658/1‑148 (MQ=255)                                                                                                             gAACAGTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCg                              >  8:94711/1‑148 (MQ=255)                                                                                                                  gTTCGCTTTCCAGCAGCTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCGTCGGt                         >  2:21/1‑148 (MQ=255)                                                                                                                      gCTTTCCAGCAGCTCGGCCGGGCTCCCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGCAATGGTATTGAAGGTTGCGCGCTACCACTTCCTTGCCGGTGACGGACTCACCCAGCATCAGCACGCTGGCGTCGGTATcc                     >  2:124019/1‑148 (MQ=255)                                                                                                                               cagcTCGGCCGGGCTAGCGCCACAGTTGACCGGCACGAACCGCGCTTCGCGGCGCTTGGAATGGTAGTGCTGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCATGATCAGCACGCTGGCGTCGTTATCGTCGACTtgc            <  6:113629/148‑1 (MQ=255)                                                                                                                                 gcTCGGCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCGTCGGTATCCGCCACTtgctg          <  6:46610/148‑1 (MQ=255)                                                                                                                                     ggCCGGGCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCGTCGGTATCCGCCACTTGCTGcatc      >  7:127945/1‑148 (MQ=255)                                                                                                                                         gggCTCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCGTCGGTATCCGCCACTTGCTGCATCATCt  >  6:37498/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                                 CAACGGCATGTCGCCGATTTCGTCGAGGAACAGGGTACCGCCGTTGGCCAGTTCGAAACGCCCGGCACGGCTGGTGATGGCCCCGGTAAAGGCGCCCTTCTCGTGGCCGAACAGTTCGCTTTCCAGCAGCTCGGCCGGGATCGCGCCACAGTTGACCGGCACGAACGGCGCTTCGCGGCGCTTGGAATGGTAGTGCAGGTTGCGCGCTACCACTTCCTTGCCGGTGCCGGACTCACCCAGGATCAGCACGCTGGCGTCGGTATCCGCCACTTGCTGCATCATCT  >  NC_002947/4964602‑4964885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑