Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,499,508	+C	intergenic (+85/‑79)	trpS → / → zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,499,506	1	.	C	100.0%	167.1 / NA	55	intergenic (+83/‑81)	trpS/zapE	tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base C (24/31);  total (24/31)

CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG  >  NC_002947/1499360‑1499649                                                                                                                                                       |                                                                                                                                                  cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑c                                                                                                                                                  <  1:150689/149‑1 (MQ=37)   cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑ccc                                                                                                                                                <  6:363195/149‑2 (MQ=37)    tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCGCCCCCGGGCATGGCACCCGGTTTGGCGGT‑GT‑TAGCGGGCCcg‑cccg                                                                                                                                               >  1:136248/1‑147 (MQ=255)        gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTcc                                                                                                                                            >  6:66359/1‑149 (MQ=25)        gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTcc                                                                                                                                            <  7:245238/149‑1 (MQ=25)        gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTcc                                                                                                                                            <  6:278801/149‑1 (MQ=25)           cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCca                                                                                                                                          >  6:87907/1‑148 (MQ=25)           cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCca                                                                                                                                          >  5:87562/1‑148 (MQ=25)               aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGAc                                                                                                                                     <  5:292494/149‑1 (MQ=21)                 gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCCCAGACt                                                                                                                                    >  8:4904/1‑148 (MQ=21)                    gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGAtt                                                                                                                                <  6:165496/149‑1 (MQ=21)                      cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGc                                                                                                                              <  5:165754/149‑1 (MQ=21)                            gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCCCGGTGCCTGGCACCGGCGTTGGCGGT‑GT‑TAGCGGGCACGCCCGCGCCCCCAGACAGATTGCCAtt                                                                                                                          >  2:220603/1‑147 (MQ=255)                                cttaaCCAAGCCTTGCCACCCCCGCCGGACTGAGGCTGCCAAACGGGGGGAGCGGGGGGGCCCGCGAAACAGCCGCCGCGGTGCAGGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                                    <  3:316582/146‑1 (MQ=18)                                cTGAACGAAGCCTTGGCACCACTGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGACCTGGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGTGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg                                                                                                                    <  1:244646/149‑1 (MQ=21)                                   aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc                                                                                                                  <  5:298614/148‑1 (MQ=21)                                        aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg                                                                                                            <  5:87907/149‑1 (MQ=21)                                           cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa                                                                                                         <  6:87562/149‑1 (MQ=21)                                                 accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa                                                                                                   >  1:48128/1‑149 (MQ=21)                                                  ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑cc                                                                                                                                                 <  5:126586/101‑1 (MQ=18)                                                    acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg                                                                                                >  5:89360/1‑149 (MQ=21)                                                                cggCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGT‑GTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt                                                                                    <  3:336998/148‑1 (MQ=25)                                                                aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg                                                                                     <  5:20116/148‑1 (MQ=21)                                                                aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                                      <  6:89360/147‑1 (MQ=21)                                                                aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt                                                                                      <  5:167507/147‑1 (MQ=21)                                                                 ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt‑‑t                                                                                 <  2:48128/149‑1 (MQ=21)                                                                    tGCCAAACGGTGGGAGCGGGCGTGCCCGCCAAAAAGGCGCCGCGGTGCATGGCACCCGCTTTGTAGGT‑GT‑TAGCGGGCACGCCCCCTCCCACAGACAGATTGCCATTAAGCGGGCCGATCGCGACAGTGACGGCCCGGTTTtttt‑‑tggc                                                                              >  8:305093/1‑147 (MQ=255)                                                                      ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGGT‑C‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGcc                                                                              <  7:176755/147‑1 (MQ=21)                                                                      ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTc                                                                            <  1:220603/149‑1 (MQ=21)                                                                      ccAAACGGGGGGCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTGGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTc                                                                            <  3:311597/149‑1 (MQ=21)                                                                            ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAAc                                                                      <  1:224437/149‑1 (MQ=21)                                                                                ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAAt                                                                  <  7:111619/149‑1 (MQ=25)                                                                                   gCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCAt                                                               <  2:230491/149‑1 (MQ=25)                                                                                      ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGAc                                                            >  8:251146/1‑149 (MQ=25)                                                                                           gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCt                                                       >  3:150709/1‑149 (MQ=37)                                                                                            cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCt                                                       <  6:279932/148‑1 (MQ=37)                                                                                              cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCt                                                       >  6:287195/1‑146 (MQ=37)                                                                                              cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAg                                                    <  2:3036/149‑1 (MQ=37)                                                                                                  aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACg                                                >  4:112128/1‑149 (MQ=37)                                                                                                   aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCCCGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGc                                               >  5:232363/1‑149 (MQ=37)                                                                                                    aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGc                                               >  3:151891/1‑148 (MQ=37)                                                                                                         cgccgcGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa                                         <  2:136248/149‑1 (MQ=37)                                                                                                          gccgcGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg                                        >  3:328815/1‑149 (MQ=37)                                                                                                           ccgcGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc                                       <  7:4904/149‑1 (MQ=37)                                                                                                             gcgGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCat                                     >  1:355100/1‑148 (MQ=37)                                                                                                                      ggcaccGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc                            >  6:314663/6‑149 (MQ=38)                                                                                                                         accGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGTGTGGGGTGTTGTTGTGACTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACg                           >  1:299479/3‑149 (MQ=25)                                                                                                                         accGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTa                                                     <  1:221333/119‑1 (MQ=37)                                                                                                                         accGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTa                                                     >  2:221333/3‑121 (MQ=37)                                                                                                                           cGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc                       >  2:55747/1‑149 (MQ=39)                                                                                                                                        gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa            <  5:66359/142‑1 (MQ=255)                                                                                                                                        gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa            <  4:151891/142‑1 (MQ=255)                                                                                                                                            tcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg          >  1:313265/4‑147 (MQ=255)                                                                                                                                              gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACttcttc              <  5:237970/140‑1 (MQ=255)                                                                                                                                              gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg       <  1:55747/147‑1 (MQ=255)                                                                                                                                              gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATAGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACttcttc              >  6:237970/2‑141 (MQ=255)                                                                                                                                                  gCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg       >  1:285602/1‑144 (MQ=255)                                                                                                                                                  gCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg       <  2:285602/144‑1 (MQ=255)                                                                                                                                                  gCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACgcggcg  >  1:52154/1‑149 (MQ=255)                                                                                                                                                       |                                                                                                                                                  CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACGCGGCG  >  NC_002947/1499360‑1499649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑