Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	336,125	+T	coding (210/237 nt)	PP_0278 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	336,124	1	.	T	98.4%	209.2 / ‑3.6	63	coding (209/237 nt)	PP_0278	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base T (25/37);  total (25/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335976‑336263                                                                                                                                                       |                                                                                                                                            gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑a                                                                                                                                              <  3:206077/149‑1 (MQ=255) gTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑a                                                                                                                                              >  3:179688/1‑149 (MQ=255)    aaCCCATCCCTCAATCTCCTACGCGTAGAGCCCTGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑tt                                                                                                                                           <  3:233710/148‑2 (MQ=255)        cATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtg                                                                                                                                       >  1:41248/1‑149 (MQ=255)         aTCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtgt                                                                                                                                      >  6:42116/1‑149 (MQ=255)          tCCCCCAACCCCCTCCGCGCCGACCCCGCCGTCCCCGTCGCCCCGGCCTACGAGCATGTCTCCATCCTGCTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCtgtg                                                                                                                                     <  2:326050/149‑1 (MQ=255)            ccTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGc                                                                                                                                    >  5:196781/1‑148 (MQ=255)             cTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGa                                                                                                                                  >  4:267332/1‑149 (MQ=255)                aaTCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGAc                                                                                                                                 <  3:267332/147‑1 (MQ=255)                      cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCCTGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCaa                                                                                                                         <  6:194697/149‑1 (MQ=255)                      cTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCaa                                                                                                                         <  3:337813/149‑1 (MQ=255)                       tACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑ttacct                                                                                                                                       >  7:116788/1‑128 (MQ=255)                       tACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑ttacct                                                                                                                                       <  8:116788/133‑6 (MQ=255)                         cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAgg                                                                                                                       <  2:41248/148‑1 (MQ=255)                         cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAgg                                                                                                                       <  8:111118/148‑1 (MQ=255)                         cgcgTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAg                                                                                                                        <  8:211609/147‑1 (MQ=255)                            gTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGGGACATGGCCAAGGCGCt                                                                                                                   >  5:240868/1‑149 (MQ=255)                             tAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtg                                                                                                                  <  6:193395/149‑1 (MQ=255)                               gagCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtga                                                                                                                 <  3:148337/148‑1 (MQ=255)                                 gCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtgatga                                                                                                              <  6:316234/149‑1 (MQ=255)                                 gCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCtgatga                                                                                                              <  8:223634/149‑1 (MQ=255)                                  cccGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGaa                                                                                                             <  7:137276/149‑1 (MQ=255)                                    cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAAc                                                                                                            >  7:32689/1‑148 (MQ=255)                                    cGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACg                                                                                                           >  4:106116/1‑149 (MQ=255)                                        gTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGatat                                                                                                       <  2:23673/149‑1 (MQ=255)                                         tCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCTCCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      <  5:52649/149‑1 (MQ=255)                                         tCCCCGTCGACGCGGCCTACGAGCATGGCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      <  3:334861/149‑1 (MQ=255)                                           cccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  6:87624/1‑147 (MQ=255)                                            ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATc                                                                                                      >  4:122850/1‑146 (MQ=255)                                            ccGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCgag                                                                                                   <  6:196781/149‑1 (MQ=255)                                                      ggCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAgg                                                                                         >  6:33713/1‑149 (MQ=255)                                                      ggCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑tt                                                                                                                                           <  1:184489/98‑2 (MQ=255)                                                      ggCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑tt                                                                                                                                           >  2:184489/1‑97 (MQ=255)                                                          tACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGt                                                                                       >  5:197418/1‑147 (MQ=255)                                                           aCGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAg                                                                                    >  4:39355/1‑149 (MQ=255)                                                            cGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAgg                                                                                   >  7:92109/1‑149 (MQ=255)                                                            cGAGCAGGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGAGGCCCAGAAGTTGCTGGGGGGAGCCG‑ATTACCTGTGCGACAGGGCCAAGGCGCTGATGATCGATTGCGAGATCGCCTAggggaggg                                                                                   >  2:284724/1‑144 (MQ=255)                                                                  tGTCTCGATCCTGATGGGCTACATCAATCACCTGGTGCGCGAGGCCGATAAGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGAGCGACATGGCCAAGGCGCAGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCAt                                                                              >  5:292178/1‑148 (MQ=255)                                                                        gataatGATTGGCTACATCAAGCACCTGGTTCGCGAGGGAGATATGGAGGACGACCATAATTTCGTGGGGCCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGc                                                                         <  6:292178/142‑1 (MQ=255)                                                                        gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       >  6:352601/1‑149 (MQ=255)                                                                        gATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAt                                                                       >  3:335939/1‑149 (MQ=255)                                                                         aTCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCAtt                                                                      <  4:179688/149‑1 (MQ=255)                                                                               aTGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCGTT‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGAcgcg                                                                <  6:188765/149‑1 (MQ=255)                                                                                tGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATTTCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCgg                                                               <  8:32689/149‑1 (MQ=255)                                                                                      aCATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCAttt                                                         >  4:260923/1‑149 (MQ=255)                                                                                        aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       <  3:122850/149‑1 (MQ=255)                                                                                        aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       >  7:308585/1‑149 (MQ=255)                                                                                        aTCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGCGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTtgt                                                       >  2:207660/1‑149 (MQ=255)                                                                                           aaGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTgg                                                     <  2:205571/148‑1 (MQ=255)                                                                                             gCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAg                                                  <  5:87624/149‑1 (MQ=255)                                                                                                 cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTaa                                                                                     <  8:64034/110‑1 (MQ=255)                                                                                                 cTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTaa                                                                                     >  7:64034/1‑110 (MQ=255)                                                                                                    gTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCtt                                           <  5:42116/149‑1 (MQ=255)                                                                                                        gcgAGGGCGATCTGGCGGACGACCATAACTTCCGGGGGTCAGCCG‑ATTCCCTGTGCGACAGGGCCAAGGCGCTGATGAACGATCTCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGGCATTTGTGGGAGCGGCCTTGCGt                                       <  8:282514/149‑1 (MQ=255)                                                                                                          gAGGGCTATATGGAGTACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTcg                                     <  3:100173/149‑1 (MQ=255)                                                                                                               cGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                <  5:352601/149‑1 (MQ=255)                                                                                                                 atatGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAAgg                              <  5:33713/149‑1 (MQ=255)                                                                                                                     ggaggaCGACCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTg                          >  6:94211/1‑149 (MQ=255)                                                                                                                       aggaCGACCATAAATTCCTGGGGGCAGCAG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAAGGGCCGc                         <  8:308585/148‑1 (MQ=39)                                                                                                                            gacCATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGACCGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagca                   <  6:240868/149‑1 (MQ=255)                                                                                                                              ccATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAagcagc                 <  2:33151/149‑1 (MQ=255)                                                                                                                               cATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGcc                                             >  3:326706/1‑120 (MQ=255)                                                                                                                               cATAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGcc                                             <  4:326706/120‑1 (MQ=255)                                                                                                                                 tAAATTCCTGGGGGCAGCCG‑ATTACCTGTGTGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                <  3:159549/131‑1 (MQ=255)                                                                                                                                 tAAATTCCTGGGGGCAGCCG‑ATTACCTGTGTGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAAt                                >  4:159549/1‑131 (MQ=255)                                                                                                                                 tAAATTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGAATTAGGCATTGACGAGGTCATTTGTGGGAGCGGCCTTGAGTCGCGAATGTGCTGCACAGCAGcccc              >  8:157093/1‑149 (MQ=255)                                                                                                                                    aTTCCTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGc           <  3:106116/149‑1 (MQ=255)                                                                                                                                        cTGGGGGCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGAt        <  1:284724/148‑1 (MQ=255)                                                                                                                                          gggggCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTc     <  4:335939/149‑1 (MQ=255)                                                                                                                                             ggCAGCCG‑ATTACCTGTGCGACATGGCCAAGGCGCTGATGAACTATATCGATATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCaga  <  7:157093/149‑1 (MQ=255)                                                                                                                                                       |                                                                                                                                            GTTAACCCATCCCTCAATCTCCTACGCGTAGAGCCCGGCGTCCCCGTCGACGCGGCCTACGAGCATGTCTCGATCCTGATGGGCTACATCAAGCACCTGGTGCGCGAGGGCGATATGGAGGACGACCATAAATTCCTGGGGGCAGCCG‑A‑TACCTGTGCGACATGGCCAAGGCGCTGATGAACGATATCGAGATCGCCAAGGGTAAGGCGCATTAGGCATTGACGCGGTCATTTGTGGGAGCGGCCTTGCGTCGCGAATGGGCTGCAAAGCAGCCCCGGCGATTTCAGA  >  NC_002947/335976‑336263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑