| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 5,674,751 | +G | intergenic (+113/+27) | rhlE‑II → / ← yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,674,750 | 1 | . | G | 98.1% | 172.1 / ‑3.4 | 52 | intergenic (+112/+28) | rhlE‑II/yceI | ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (26/25); total (27/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.44e-01 | |||||||||||
GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC > NC_002947/5674603‑5674890 | ggTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑g < 1:143385/149‑1 (MQ=255) aGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccc > 5:280097/1‑145 (MQ=255) cgcCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAgc‑ggccccc > 4:300148/1‑143 (MQ=255) gcgcTGGTGACCCGCAATGGCTGATAGAAGTACCGTGGGGGCTGCTTCGCAGCCCTATCACGACACATGGCCGCTCCTGCAGAGTATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGtt < 4:4848/149‑1 (MQ=37) cgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGttt > 1:174822/1‑149 (MQ=255) aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCt < 4:317836/147‑1 (MQ=255) ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTgg > 5:181362/1‑147 (MQ=255) cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAg < 2:240098/149‑1 (MQ=255) gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAg > 6:318246/1‑148 (MQ=255) gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGa < 2:174822/149‑1 (MQ=255) aGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAAt > 4:122904/1‑149 (MQ=255) aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACtt < 8:60110/147‑1 (MQ=255) aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag < 3:110455/149‑4 (MQ=25) aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCGTGATCTCTAGAGGAGCGGCCTTGTGTCGTGATAGGGCCGCGAAGC‑GGGCCCCGCGGTTGTCAGCTGGGAGAAATTACTTCTGGTTc > 2:66414/1‑149 (MQ=25) gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCt < 1:240609/149‑1 (MQ=255) atagggCTGCGAAGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTGCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt > 3:159107/4‑141 (MQ=25) atagggCTGCGAAGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt < 4:159107/138‑1 (MQ=37) ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa > 4:354393/1‑148 (MQ=255) ctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac < 3:114033/149‑1 (MQ=255) ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTTCTTCACACCTTCGACACTGa > 2:136765/1‑149 (MQ=255) aGCCCTATCACGACACAAGGCCGCTCCAGCAGAGAATTTCTTGATGTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGGTTTCAGCTGGCAGAAATTACTTATGCTTCACACCTTCGACACTGATAtc > 1:200578/1‑147 (MQ=37) tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag > 1:262012/1‑149 (MQ=255) aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg > 6:317959/1‑149 (MQ=255) aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg > 1:249684/1‑149 (MQ=255) caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg < 6:181362/149‑1 (MQ=255) aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa < 3:354393/149‑1 (MQ=255) cAGAGAATTTCTTGATCTCTCCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg < 4:278571/147‑1 (MQ=255) tttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa > 4:182133/1‑149 (MQ=255) tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt < 5:317959/148‑1 (MQ=255) tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt < 2:262012/148‑1 (MQ=255) gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc < 1:136765/147‑1 (MQ=255) gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACCGATATCCAGGTCCAGGGTc < 6:195077/118‑1 (MQ=38) gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACAGATATCCAGGTCCAGGGTc > 5:195077/1‑118 (MQ=38) aTCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg > 4:317438/1‑147 (MQ=255) tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa < 3:122904/148‑1 (MQ=255) tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg > 7:53209/1‑149 (MQ=255) ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg < 5:84514/112‑1 (MQ=255) ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg > 6:84514/1‑112 (MQ=255) tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCAAGGTCCAGGGACTGCGAAGTAGCACCCGGGCCATTGATGCCGAAGTc > 8:107487/1‑149 (MQ=38) tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgt > 1:230461/1‑149 (MQ=255) cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt < 2:249684/148‑1 (MQ=255) aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt > 2:26534/1‑147 (MQ=255) gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg < 2:355349/117‑1 (MQ=255) aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc > 4:262053/1‑73 (MQ=25) ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca < 3:193209/149‑1 (MQ=255) ccTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc > 3:84606/1‑149 (MQ=255) ttGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc > 1:140656/1‑128 (MQ=255) ttGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc < 2:140656/128‑1 (MQ=255) gtgtCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg < 2:230461/148‑1 (MQ=255) tCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg > 6:231476/1‑149 (MQ=255) gTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg > 2:21876/1‑149 (MQ=255) aTAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCg > 7:48802/1‑149 (MQ=255) tAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCg < 8:53209/148‑1 (MQ=255) gCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa < 1:66414/149‑1 (MQ=255) cAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc > 3:294140/1‑147 (MQ=255) | GGTGAGCGCCCAGCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC > NC_002947/5674603‑5674890 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |