| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,126,647 | +C | intergenic (‑101/+48) | tdcG‑II ← / ← gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,126,645 | 1 | . | C | 88.6% | 101.8 / 8.1 | 35 | intergenic (‑99/+50) | tdcG‑II/gcvP‑I | L‑serine dehydratase/glycine dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base . (3/1); new base C (8/23); total (11/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAA‑CC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGC > NC_002947/1126500‑1126782 | gAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAA‑c < 6:249022/147‑1 (MQ=255) gccgatgccgatCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTTACCCCGAAT‑GCGGAGGGATTGGCACCATAGCATTAG‑C‑GGGGAAA‑cccgcacc > 2:297414/1‑143 (MQ=255) tCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGCGGGGTTTACCC‑CGCATGGGGGGGGTTTAGCC‑CCAACCCAATAGCC‑GGGTAAA‑CC‑GCGCCGAAACAAAGcccccccccccac > 2:210510/1‑146 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCggg < 1:210510/149‑1 (MQ=255) aGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCgg < 8:106073/148‑1 (MQ=255) tGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGa < 1:260193/149‑1 (MQ=255) tGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGc < 1:198823/120‑1 (MQ=255) ccAGGTTGTTGGGTGGACATGGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTcc < 7:136086/147‑1 (MQ=255) cAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGa < 3:91081/149‑1 (MQ=255) gttgttGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAg < 1:297414/149‑1 (MQ=255) gttgGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGACGCCTGATAGcc < 2:314580/148‑2 (MQ=38) gggTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCtctc < 4:242833/148‑1 (MQ=255) gTGGACATTGAGCCCTGTAGGCGC‑GGGTTTAACCGCGCAT‑GCGGAGGTTTAGCCCCCCTCGCCATAG‑CGGGGTAAC‑CC‑CCGCCGACACAGAGCCCCCCCCCCCAGCCAGGTGGCGGGGGGGGCG‑ATAAGGGGGGCGGATAGGGCtcgat > 2:286072/1‑149 (MQ=255) ggACATTGAGCCCTGTAGGCGC‑GGGTTTAACCCCCAAA‑TCGGAGGTATAGCCACCCAAGCAATAG‑CGGGGAAAA‑CC‑CCACCAAAACACAACCCCCCCCCCCAACCACGGGGCGGGGCGGGCT‑ATAAAGCGTCCTGATAgagatagc > 8:18844/1‑139 (MQ=255) taCATTTAGCCCTTTTGGCGC‑GGGTATACCCGATAAT‑GCGGTGGTATAGCCACCTTCGCATCCG‑C‑GGGAAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCtcgatcgt < 8:299168/148‑2 (MQ=255) ttGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACggg < 7:68010/149‑1 (MQ=255) gCCCTGTAGGCGC‑GGGTTTACCCGCTAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAgg < 7:43854/149‑1 (MQ=255) gTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGc < 7:18844/149‑1 (MQ=255) ccGCTAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc < 4:76238/149‑1 (MQ=255) ccGCGAAT‑GCGGCGGTATCGCCCCCATCGCATTCG‑C‑GGTTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc < 6:194140/149‑1 (MQ=255) ccGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTcc < 4:158996/103‑1 (MQ=255) ccGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg > 3:356779/1‑144 (MQ=255) ccGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCg < 4:356779/144‑1 (MQ=255) ccGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGc > 8:296071/1‑149 (MQ=255) cgAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCTCTTCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCg < 1:286072/149‑1 (MQ=39) gTATAGCCACCATCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAAGAGGTTGtg < 2:59205/148‑2 (MQ=255) acctccGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGcc > 7:331051/6‑149 (MQ=255) accaTCCCATCCG‑C‑GGGTAAA‑CCAGCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGcc < 8:170427/149‑1 (MQ=255) caTCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCACGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGa > 1:95911/1‑149 (MQ=255) aTCGCATTCG‑G‑GGTAAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCTGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAc < 2:176968/149‑1 (MQ=255) tCGCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGAcc > 4:347459/1‑149 (MQ=255) gCATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACcgg > 4:76239/1‑149 (MQ=255) cATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGACGCCTGATAg > 6:264250/1‑80 (MQ=37) cATTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGACGCCTGATAg < 5:264250/80‑1 (MQ=37) aTTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt > 4:312166/1‑116 (MQ=255) aTTCG‑C‑GGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGt < 3:312166/116‑1 (MQ=255) cg‑c‑gGGTAAACCC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCggcggc < 3:234797/148‑1 (MQ=255) | GAGGGGCCGATGCCGATCTTGAACAGGTCGAAGACGCTCAGTGACATGTGGACCTCCTGCCAGGTTGTTGGGTGGACATTGAGCCCTGTAGGCGC‑GGGTTTACCCGCGAAT‑GCGGAGGTATAGCCACCATCGCATTCG‑C‑GGGTAAA‑CC‑GCTCCTACACAGAGCCCGCCACCCAAGCCAGGTGGCGGGGAGGGCT‑ATCAGGCGTCCTGATAGCTCTCGATCGACGGGCAGGCGCACACCAGGTTGCGGTCGCCGAACACGTTGTCGACCCGGCCGACCGGCGGC > NC_002947/1126500‑1126782 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |