| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 196,879 | 0 | G | A | 35.3% | 17.5 / 10.7 | 17 | V795V (GTG→GTA) | PP_0168 | putative surface adhesion protein |
| Reads supporting (aligned to +/- strand): ref base G (6/5); new base A (3/3); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGT > NC_002947/196732‑196937 | cGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGa > 8:140023/1‑149 (MQ=32) tCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATc < 2:297306/149‑1 (MQ=32) cGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGCACCCGACGCCGGCTCAGACCACGATCAACGCCTCGGTCGA‑TGCCACCCCCGCGACCCTGACGGCGAGCCCGTCGGTCCCCGAAGGCGGCGTGATCAcc > 3:200517/1‑148 (MQ=255) cATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCg > 1:1762/1‑149 (MQ=32) tCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCACGAACCCGACGCCGGCTCAGACCAAGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTg < 4:140987/149‑1 (MQ=17) agaACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGAcc > 8:245576/1‑149 (MQ=33) aCGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTg < 7:245576/149‑1 (MQ=34) cGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGa < 2:1762/149‑1 (MQ=34) atcccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACTCCGGTGACa > 7:240200/3‑147 (MQ=2) aCCCCGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACa < 3:359617/147‑1 (MQ=11) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGACTGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGt > 1:258592/1‑148 (MQ=17) cccGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGa > 5:126095/1‑149 (MQ=32) ccGAACCCGACCCCGGCTCAGACCACGATCAACTACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc < 2:47234/149‑1 (MQ=12) ccGAACCCGACCCCGGCTCAGACCACGATCAACGCCTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTACTCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc > 6:193549/1‑149 (MQ=255) ccGAACCCGACCCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAc > 5:96601/1‑149 (MQ=17) cGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGAcc < 5:35012/149‑1 (MQ=32) cccGACCCCGGTTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTAATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGt < 6:354760/149‑1 (MQ=2) | CGTCAGCGTCACCATCGAGAACGCCACTGGCGGCAACTTCGAACAGCTGACCCCGAACCCGACGCCGGCTCAGACCACGATCAACGACTCGGTCGA‑TGCCACCACCGCGACCCTGACGGCGAGCCCGTCGGTCACCGAAGGCGGCGTGATCACCTACACCGTGACCCTGAGCAATCCTGCCCAGACGCCGGTGACAGTGACCCTGT > NC_002947/196732‑196937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |