Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	2,921,460	G→A	G3494R (GGG→AGG)	PP_2561 →	hemolysin‑type calcium‑binding bacteriocin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,921,460	0	G	A	100.0%	131.0 / NA	40	G3494R (GGG→AGG)	PP_2561	hemolysin‑type calcium‑binding bacteriocin
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (22/18);  total (22/18)

ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAA  >  NC_002947/2921314‑2921602                                                                                                                                                   |                                                                                                                                                  tgtcGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg                                                                                                                                                 <  8:221131/146‑1 (MQ=255) acacGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCagg                                                                                                                                                 <  8:295040/149‑1 (MQ=255)         cTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGAGAGGACAGCCTGGATGGCGGCAACGGCAATGACAACCTGCTGGGCTTCGATTGTAACGACAGATTGTTCTGCGACTTGGGTACCGACACACTGATCATGGGGGcct                                                                                                                                          >  1:214673/1‑147 (MQ=255)                   ccGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                               <  7:514/149‑1 (MQ=255)                         atctcAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAAc                                                                                                                         <  7:307694/145‑1 (MQ=255)                              aaCCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACATCCTCAAc                                                                                                                         >  8:307694/1‑144 (MQ=255)                                 cTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCgg                                                                                                                  >  7:33560/1‑148 (MQ=255)                                                   gATGAGAGCCTGGATGGCGGCAACGGCATTTACACCCTGCAGGGCGGCGTTGGAAACGAGACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑ggtggt                                                                                            <  2:45572/149‑1 (MQ=255)                                                   gATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑ggtggt                                                                                            >  1:157693/1‑149 (MQ=255)                                                   gATGACAGCCTGGATGCCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑ggtggt                                                                                            <  8:205345/149‑1 (MQ=255)                                                      gACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTggtggg                                                                                         >  1:211594/1‑149 (MQ=255)                                                                aTGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                               >  3:254246/1‑104 (MQ=255)                                                                aTGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTc                                                                                                                               <  4:254246/104‑1 (MQ=255)                                                                  ggcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCgg                                                                                <  6:103675/146‑1 (MQ=255)                                                                  ggcggcAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCgg                                                                                >  5:103675/1‑146 (MQ=255)                                                                         acggcaaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGAATTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACAcc                                                                      <  2:157693/149‑1 (MQ=255)                                                                              aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTcc                                                                                                                              >  4:87367/1‑91 (MQ=255)                                                                              aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTcc                                                                                                                              <  3:87367/91‑1 (MQ=255)                                                                              aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAAc                                                                            >  4:234842/1‑138 (MQ=255)                                                                              aaTGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAAc                                                                            <  3:234842/138‑1 (MQ=255)                                                                                    acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTCGGGCCGGTAACGACACCATGATCGCCAc                                                           >  2:118346/1‑149 (MQ=255)                                                                                    acCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGCTGGGGCCGGTAACGACACCATGATCGacac                                                           >  5:98289/1‑149 (MQ=255)                                                                                      ccTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCAcc                                                          >  1:341342/1‑148 (MQ=255)                                                                                       cTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACGCCATGATCGCCACCGa                                                        >  2:181451/1‑149 (MQ=255)                                                                                                ggcgATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGa                                               >  8:309976/1‑149 (MQ=255)                                                                                                       gTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACAcc                                                                      <  8:37612/119‑1 (MQ=255)                                                                                                       gTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACAcc                                                                      >  7:37612/1‑119 (MQ=255)                                                                                                                    gTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                           >  6:29066/1‑149 (MQ=255)                                                                                                                    gTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                           >  1:190034/1‑149 (MQ=255)                                                                                                                    gTTCGGCGACGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAg                           >  1:346807/1‑149 (MQ=255)                                                                                                                            aCGTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCCCTGCAGGCTTTGGc                   <  7:309976/149‑1 (MQ=255)                                                                                                                              gTGGGTACCGACACCCTGCTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCaa                 <  2:299068/149‑1 (MQ=255)                                                                                                                              gTGGGTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACGCCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCaa                 <  1:181451/149‑1 (MQ=255)                                                                                                                                 ggTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAACCGATGTGTTCGAGTTCGCTGCAGGCTTTGGCACCGa              >  8:188206/1‑149 (MQ=255)                                                                                                                                  gTACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGAc             <  2:341342/149‑1 (MQ=255)                                                                                                                                   tACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg                    >  8:124862/1‑141 (MQ=255)                                                                                                                                   tACCGACACCCTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTgg                    <  7:124862/141‑1 (MQ=255)                                                                                                                                           cccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc    <  2:211594/149‑1 (MQ=255)                                                                                                                                            ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcatc    >  3:318878/1‑148 (MQ=255)                                                                                                                                            ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat     >  7:297984/1‑147 (MQ=255)                                                                                                                                            ccTGATCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGatcat     <  8:297984/147‑1 (MQ=255)                                                                                                                                                aTCAGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATCCTCACGTGTGGGGTTGGGCCTTATACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCaa  <  1:118346/149‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  ACACGTTGCTGGGGGAGGCCGGTAACGACAACCTGAACGGGGGCGCTGGCGATGACAGCCTGGATGGCGGCAACGGCAATGACACCCTGCAGGGCGGCGATGGTAACGACACATTGTTCGGCGACGTGGGTACCGACACCCTGATCGGGGGGGCCGGCAACGACTTCCTCAACGGTGCGGCAGGTAACGATAC‑‑‑GGTGGTGGGTGGGGCCGGTAACGACACCATGATCGCCACCGATGGAAACGATGTGTTCCAGTTCGCTGCAGGCTTTGGCAACGACCTGATCATCAA  >  NC_002947/2921314‑2921602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑