Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,674,753 G→C intergenic (+115/+25) rhlE‑II → / ← yceI ATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,674,7530GC98.1% 175.1 / NA 52intergenic (+115/+25)rhlE‑II/yceIATP‑dependent RNA helicase/polyisoprene‑binding acidic stress response factor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base C (26/25);  total (27/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674615‑5674890
                                                                                                                                           |                                                                                                                                          
gcgcTGGTGACCCGCAATGGCTGATAGAAGTACCGTGGGGGCTGCTTCGCAGCCCTATCACGACACATGGCCGCTCCTGCAGAGTATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGtt                                                                                                                                  <  4:4848/149‑1 (MQ=37)
 cgcTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGttt                                                                                                                                 >  1:174822/1‑149 (MQ=255)
         aCCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCt                                                                                                                           <  4:317836/147‑1 (MQ=255)
           ccGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTgg                                                                                                                         >  5:181362/1‑147 (MQ=255)
            cGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAg                                                                                                                      <  2:240098/149‑1 (MQ=255)
             gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAg                                                                                                                      >  6:318246/1‑148 (MQ=255)
             gCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGa                                                                                                                     <  2:174822/149‑1 (MQ=255)
                aGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAAt                                                                                                                  >  4:122904/1‑149 (MQ=255)
                       aTAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACtt                                                                                                             <  8:60110/147‑1 (MQ=255)
                            aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAGTAATTTCTGccag                                                                                                      <  3:110455/149‑4 (MQ=25)
                            aGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCGTGATCTCTAGAGGAGCGGCCTTGTGTCGTGATAGGGCCGCGAAGC‑GGGCCCCGCGGTTGTCAGCTGGGAGAAATTACTTCTGGTTc                                                                                                      >  2:66414/1‑149 (MQ=25)
                                  gAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCt                                                                                                <  1:240609/149‑1 (MQ=255)
                                   atagggCTGCGAAGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTGCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       >  3:159107/4‑141 (MQ=25)
                                   atagggCTGCGAAGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCtt                                                                                                       <  4:159107/138‑1 (MQ=37)
                                       ggCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGa                                                                                            >  4:354393/1‑148 (MQ=255)
                                         ctgctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGacac                                                                                         <  3:114033/149‑1 (MQ=255)
                                            ctTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTTCTTCACACCTTCGACACTGa                                                                                      >  2:136765/1‑149 (MQ=255)
                                                  aGCCCTATCACGACACAAGGCCGCTCCAGCAGAGAATTTCTTGATGTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGGTTTCAGCTGGCAGAAATTACTTATGCTTCACACCTTCGACACTGATAtc                                                                                  >  1:200578/1‑147 (MQ=37)
                                                         tCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccag                                                                         >  1:262012/1‑149 (MQ=255)
                                                           aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                       >  6:317959/1‑149 (MQ=255)
                                                           aCGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAggg                                                                       >  1:249684/1‑149 (MQ=255)
                                                                 caAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCg                                                                 <  6:181362/149‑1 (MQ=255)
                                                                   aGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGaa                                                               <  3:354393/149‑1 (MQ=255)
                                                                               cAGAGAATTTCTTGATCTCTCCAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCg                                                     <  4:278571/147‑1 (MQ=255)
                                                                                      tttCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGa                                            >  4:182133/1‑149 (MQ=255)
                                                                                        tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                           <  5:317959/148‑1 (MQ=255)
                                                                                        tCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGAt                                           <  2:262012/148‑1 (MQ=255)
                                                                                            gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        <  1:136765/147‑1 (MQ=255)
                                                                                            gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACCGATATCCAGGTCCAGGGTc                                                                     <  6:195077/118‑1 (MQ=38)
                                                                                            gATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACAGATATCCAGGTCCAGGGTc                                                                     >  5:195077/1‑118 (MQ=38)
                                                                                             aTCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCg                                       >  4:317438/1‑147 (MQ=255)
                                                                                              tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGaa                                     <  3:122904/148‑1 (MQ=255)
                                                                                              tctcTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAg                                    >  7:53209/1‑149 (MQ=255)
                                                                                               ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                        >  6:84514/1‑112 (MQ=255)
                                                                                               ctctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATAtccaggtccagg                                                                        <  5:84514/112‑1 (MQ=255)
                                                                                                tctACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCAAGGTCCAGGGACTGCGAAGTAGCACCCGGGCCATTGATGCCGAAGTc                                  >  8:107487/1‑149 (MQ=38)
                                                                                                  tACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgt                                >  1:230461/1‑149 (MQ=255)
                                                                                                    cAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               <  2:249684/148‑1 (MQ=255)
                                                                                                     aGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgtt                               >  2:26534/1‑147 (MQ=255)
                                                                                                       gAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAg                                                           <  2:355349/117‑1 (MQ=255)
                                                                                                        aGCGGCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTc                                                                                                      >  4:262053/1‑73 (MQ=25)
                                                                                                           ggCCTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCgttcaggttca                       <  3:193209/149‑1 (MQ=255)
                                                                                                             ccTTGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGc                     >  3:84606/1‑149 (MQ=255)
                                                                                                               ttGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        >  1:140656/1‑128 (MQ=255)
                                                                                                               ttGTGTCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGcc                                        <  2:140656/128‑1 (MQ=255)
                                                                                                                 gtgtCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtg                  <  2:230461/148‑1 (MQ=255)
                                                                                                                    tCGTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtgg              >  6:231476/1‑149 (MQ=255)
                                                                                                                      gTGATAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCgtggtggtg            >  2:21876/1‑149 (MQ=255)
                                                                                                                         aTAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCg         >  7:48802/1‑149 (MQ=255)
                                                                                                                          tAGGGCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCg         <  8:53209/148‑1 (MQ=255)
                                                                                                                              gCCGCAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGaa    <  1:66414/149‑1 (MQ=255)
                                                                                                                                  cAAAGCGGCC‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGc  >  3:294140/1‑147 (MQ=255)
                                                                                                                                           |                                                                                                                                          
GCGCTGCTGACCCGCAAGGGCTGATAGAAGTACCGAGGGGGCTGCTTCGCAGCCCTATCACGACACAAGGCCGCTCCTGCAGAGAATTTCTTGATCTCTACAGGAGCGGCCTTGTGTCGTGATAGGGCCGCAAAGC‑GCG‑CCCTCGGTTTTCAGCTGGCAGAAATTACTTCTGCTTCACACCTTCGACACTGATATCCAGGTCCAGGGTCTGCGAAGTAGCACCCGGGCCTTTGATGCCGAAGTCGTTCAGGTTCAGCGTGGTGGTGGCGTTGAAGC  >  NC_002947/5674615‑5674890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑