Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	5,986,841	T→A	intergenic (+121/‑78)	PP_5245 → / → kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,986,841	0	T	A	100.0%	127.4 / NA	38	intergenic (+121/‑78)	PP_5245/kefB‑III	AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (19/19);  total (19/19)

GGTGGCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCC  >  NC_002947/5986697‑5986972                                                                                                                                                 |                                                                                                                                    ggtggCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGa                                                                                                                                 >  6:331155/1‑149 (MQ=255)   tggCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACg                                                                                                                               <  2:29878/149‑1 (MQ=255)               gTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTAcccc                                                                                                                   <  4:3515/149‑1 (MQ=255)                tCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt                                                                                                                  <  4:295943/149‑1 (MQ=255)                  cGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCt                                                                                                                  >  2:135587/1‑147 (MQ=255)                  cGATTAGCGCGGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCa                                                                                                                <  2:204093/149‑1 (MQ=255)                        gcgcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTg                                                                                                          >  4:187724/1‑149 (MQ=255)                          gcTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTc                                                                                                        <  2:254948/149‑1 (MQ=255)                               cttcttCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGCTTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTc                                                                                                    >  1:327827/1‑148 (MQ=255)                                 tgggagcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCaaa                                                                                                 >  3:23741/6‑149 (MQ=255)                                            gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAg                                                                                                               <  3:352066/124‑1 (MQ=255)                                            gcgcCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAg                                                                                                               >  4:352066/1‑124 (MQ=255)                                               cccGCTCCCACAGCGATAGCACAGGCCTTGCGCCCAGGGACGCGCCGGCACCGATTGTCACTCTGGACAGGACAAATTTTCATCCCGTCAGATGATAGACTGACGCCACACTCCCCCGCAGATGTGTCCGTCAAACTGTTCGAACGCTg                                                                                   >  4:10225/1‑149 (MQ=255)                                                 cgatctcacaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGcc                                                                                 <  7:289825/143‑1 (MQ=255)                                                       cacaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg                                                                             >  4:14473/1‑147 (MQ=255)                                                        acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGcc                                                                                 >  8:289825/1‑142 (MQ=255)                                                        acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAg                                                                          >  7:95512/1‑149 (MQ=255)                                                              aTAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACACTGCCGGGGCAGcgttcg                                                                    <  5:331155/149‑6 (MQ=255)                                                                aGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa                                                                  <  7:241590/149‑1 (MQ=255)                                                                              gCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACt                                                                                                                                   >  3:165706/1‑70 (MQ=255)                                                                              gCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACt                                                                                                                                   <  4:165706/70‑1 (MQ=255)                                                                                       aGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGaa                                            <  6:82934/148‑1 (MQ=255)                                                                                       aGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGaa                                            <  4:23741/148‑1 (MQ=255)                                                                                            cGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGc                                      <  8:95512/149‑1 (MQ=255)                                                                                              gCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCGGGGCAGcgttcg                                                                    <  4:48738/117‑6 (MQ=255)                                                                                              gCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCGGGGCAGcgttcg                                                                    >  3:48738/1‑112 (MQ=255)                                                                                               cACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGaa                                                                                                             >  4:211063/1‑75 (MQ=255)                                                                                               cACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGaa                                                                                                             <  3:211063/75‑1 (MQ=255)                                                                                                aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATAATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc                                  >  1:258394/1‑149 (MQ=255)                                                                                                 ccGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCt                                 <  1:135587/149‑1 (MQ=255)                                                                                                    aTTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGc                               >  4:151641/1‑148 (MQ=255)                                                                                                           ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg                       <  3:187724/149‑1 (MQ=255)                                                                                                                gacaggacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCGTCAGAATTGTCGGTCAAACTGTTAGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGa                   >  8:343096/1‑148 (MQ=255)                                                                                                                     gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCt                                                                        <  7:45593/90‑1 (MQ=255)                                                                                                                     gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCt                                                                        >  8:45593/1‑90 (MQ=255)                                                                                                                     gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc             <  3:14473/149‑1 (MQ=255)                                                                                                                     gacaATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc             >  3:115593/1‑149 (MQ=255)                                                                                                                                ttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc  >  6:274900/1‑149 (MQ=255)                                                                                                                                                 |                                                                                                                                    GGTGGCGGTGAACAGTCCGATTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCC  >  NC_002947/5986697‑5986972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑