| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS → / → zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,499,497 | 1 | . | C | 93.8% | 120.1 / ‑0.6 | 50 | intergenic (+74/‑90) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base . (0/2); major base C (18/27); minor base A (3/0); total (21/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.33e-01 | |||||||||||
| * | NC_002947 | 1,499,498 | 0 | T | G | 100.0% | 148.4 / NA | 50 | intergenic (+75/‑89) | trpS/zapE | tryptophan‑‑tRNA ligase/nucleoside triphosphate hydrolase domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (21/29); total (21/29) | |||||||||||
CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499360‑1499644 || cGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑c < 1:150689/149‑1 (MQ=37) cTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑ccc < 6:363195/149‑2 (MQ=37) tGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGCGAAACAGGCGCCCCCGGGCATGGCACCCGGTTTGGCGGT‑GT‑TAGCGGGCCcg‑cccg > 1:136248/1‑147 (MQ=255) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACCGGCGCCGCGGTGCATGGCCCCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTcc > 6:66359/1‑149 (MQ=25) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTcc < 7:245238/149‑1 (MQ=25) gCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTcc < 6:278801/149‑1 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCca > 6:87907/1‑148 (MQ=25) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCca > 5:87562/1‑148 (MQ=25) aTGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGAc < 5:292494/149‑1 (MQ=21) gCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCCCAGACt > 8:4904/1‑148 (MQ=21) gCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAAGGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGAtt < 6:165496/149‑1 (MQ=21) cATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGc < 5:165754/149‑1 (MQ=21) gagCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAGGCGCCCCGGTGCCTGGCACCGGCGTTGGCGGT‑GT‑TAGCGGGCACGCCCGCGCCCCCAGACAGATTGCCAtt > 2:220603/1‑147 (MQ=255) cttaaCCAAGCCTTGCCACCCCCGCCGGACTGAGGCTGCCAAACGGGGGGAGCGGGGGGGCCCGCGAAACAGCCGCCGCGGTGCAGGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 3:316582/146‑1 (MQ=18) cTGAACGAAGCCTTGGCACCACTGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGACCTGGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGTGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 1:244646/149‑1 (MQ=21) aaCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGc < 5:298614/148‑1 (MQ=21) aGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 5:87907/149‑1 (MQ=21) cTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa < 6:87562/149‑1 (MQ=21) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGa > 1:48128/1‑149 (MQ=21) ccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCAcg‑cc < 5:126586/101‑1 (MQ=18) acaGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACgg > 5:89360/1‑149 (MQ=21) cggCTGCCAAACGGTGGGAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCAGGGCACCGGCTTTGCCGGT‑GTGC‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt < 3:336998/148‑1 (MQ=25) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttg < 5:20116/148‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 5:167507/147‑1 (MQ=21) aGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGtttttt < 6:89360/147‑1 (MQ=21) ggCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTttgt‑‑t < 2:48128/149‑1 (MQ=21) tGCCAAACGGTGGGAGCGGGCGTGCCCGCCAAAAAGGCGCCGCGGTGCATGGCACCCGCTTTGTAGGT‑GT‑TAGCGGGCACGCCCCCTCCCACAGACAGATTGCCATTAAGCGGGCCGATCGCGACAGTGACGGCCCGGTTTtttt‑‑tggc > 8:305093/1‑147 (MQ=255) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGTGGT‑C‑GCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGcc < 7:176755/147‑1 (MQ=21) ccAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTc < 1:220603/149‑1 (MQ=21) ccAAACGGGGGGCGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTGGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTc < 3:311597/149‑1 (MQ=21) ggTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAAc < 1:224437/149‑1 (MQ=21) ggAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAAt < 7:111619/149‑1 (MQ=25) gCGGGCGTGCCCGCTAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCAt < 2:230491/149‑1 (MQ=25) ggCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGAc > 8:251146/1‑149 (MQ=25) gCCCGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCt > 3:150709/1‑149 (MQ=37) cccGCGAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCt < 6:279932/148‑1 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCt > 6:287195/1‑146 (MQ=37) cgcgAAACAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAg < 2:3036/149‑1 (MQ=37) aaaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACg > 4:112128/1‑149 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCCCGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 5:232363/1‑149 (MQ=37) aCAGGCGCCGCGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGc > 3:151891/1‑148 (MQ=37) cgccgcGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCaa < 2:136248/149‑1 (MQ=37) gccgcGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 3:328815/1‑149 (MQ=37) ccgcGGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGc < 7:4904/149‑1 (MQ=37) gcgGTGCATGGCACCGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCat > 1:355100/1‑148 (MQ=37) ggcaccGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAAc > 6:314663/6‑149 (MQ=38) accGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGTGTGGGGTGTTGTTGTGACTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACg > 1:299479/3‑149 (MQ=25) accGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTa > 2:221333/3‑121 (MQ=37) accGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTa < 1:221333/119‑1 (MQ=37) cGGCTTTGCCGGT‑GT‑TCGCGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc > 2:55747/1‑149 (MQ=39) gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa < 5:66359/142‑1 (MQ=255) gtgttcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCa < 4:151891/142‑1 (MQ=255) tcgcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATg > 1:313265/4‑147 (MQ=255) gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACttcttc < 5:237970/140‑1 (MQ=255) gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGAcg < 1:55747/147‑1 (MQ=255) gcGGGCACGCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATAGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACttcttc > 6:237970/2‑141 (MQ=255) || CGCTGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCACGGCTTTGCCGGT‑GT‑T‑TCGGGCACG‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGT‑‑TGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACTTCTTCCATGACG > NC_002947/1499360‑1499644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |