| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,932,640 | +C | intergenic (‑107/+43) | rluA ← / ← minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,932,638 | 1 | . | C | 92.7% | 130.1 / 4.6 | 41 | intergenic (‑105/+45) | rluA/minE | bifunctional tRNA pseudouridine(32) synthase TrmQ/ribosomal large subunit pseudouridine synthase RluA/cell division topological specificity factor |
| Reads supporting (aligned to +/- strand): ref base . (3/0); new base C (19/19); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01 | |||||||||||
ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932490‑1932781 | aTGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑g < 4:123061/149‑1 (MQ=255) cgTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑cccgcg > 6:123881/1‑144 (MQ=255) aaGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTg > 7:118142/1‑148 (MQ=255) tCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAAtt > 5:323549/1‑149 (MQ=255) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGc > 6:170468/1‑136 (MQ=255) gACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGc < 5:170468/136‑1 (MQ=255) accggggCATTGCTGGTGTCACTAAAATCGCCTTAACGGGGGGGGCGGTTTGGGGGCCCTTCGGGGGAACGCCGGCCCCAAAAATGTTTATGGGTCCCCGGTCGCTGG‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 6:323549/144‑1 (MQ=255) aCAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTc < 8:118142/149‑1 (MQ=255) aGCGGCATTGCAGGTCTCACTAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGa < 7:229633/149‑1 (MQ=255) actaaaaTCCCAGTAACCGCGGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGG‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATc < 4:289397/146‑1 (MQ=37) aCGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCCGCCCCAAATTGTCGCCTACGCGGATCAGCGATc > 3:46924/1‑149 (MQ=37) gAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCCCTCCTACAAAGGATATTCGGACCCTGTAGGAGC‑GGGCTTGG‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTTTCGACTACGCCGATAAGCGATCCg > 8:95794/1‑149 (MQ=25) aaaaTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCgg < 5:123881/149‑1 (MQ=255) aaaTCGCAGTAAACGCTGGGGCCGCTTGGCGGCCCTGCGCGGGCAACCCCGCTCCTACAAAGGATATGCGTCCCCTGTCGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCg < 1:141811/147‑1 (MQ=38) aTAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTAAAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAg > 5:129067/1‑149 (MQ=255) aGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAggg > 5:114296/1‑149 (MQ=255) aGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCGACAAAGGATATGCGTGCCCTGTAGGAGC‑GGGGTT‑GCCC‑GCGCAGGGCGGCCAGGCAGCCCCAAATTGTCGACTACGCGGGTCAGCGCACCGGCAGGGTGa > 4:173524/1‑149 (MQ=37) aaCGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAACCCCCTACGAAAAAGGATATGAGGGACCGGTAGGAGCGGGGTTT‑G‑CC‑GCGAAAGGCTGGAAGGCCGCCCCAAAATGTGGACAACCCAGCTACGCGGTCCGGCAGGGGGATGtt > 6:22677/1‑149 (MQ=255) gcggcggCCGCTTTGCGGCCCGTCGCGGGCAAGCCCGCTCCTACAAAGCATATGCGCCCCCTGTCGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc < 4:284008/142‑1 (MQ=38) gCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTc > 3:284008/1‑147 (MQ=255) cTGGGGCCGCTTTGCGGCCCTTCGCGGGCAACCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGc < 7:5691/149‑1 (MQ=255) gggcCCGCTTCGCGCCCCTGGCCGGCCACCCCCGCCCCAAAAACGTATATGCGCCCCGTGTCGCCGG‑GGGCTG‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTc < 7:130082/149‑1 (MQ=255) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg < 8:176655/134‑1 (MQ=255) cTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATg > 7:176655/1‑134 (MQ=255) gCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGag < 7:95794/148‑1 (MQ=255) tCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGccc > 4:350713/1‑149 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTg > 2:93121/1‑144 (MQ=255) gcgGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTg < 1:93121/144‑1 (MQ=255) gCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGttt > 5:154768/1‑149 (MQ=255) cccGCTCCTACAAAGGATATGCGTCCCCGGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCACGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAg > 4:351892/1‑149 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt > 8:69486/1‑144 (MQ=255) ccGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGtttt < 7:69486/144‑1 (MQ=255) cTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGata > 1:254741/1‑149 (MQ=255) aCAAAGCATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATg < 3:173524/149‑1 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc > 3:276240/1‑136 (MQ=255) aaaGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTcc < 4:276240/136‑1 (MQ=255) atGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc > 5:361381/1‑149 (MQ=255) tGCGTCCCCTGTAGGAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACatcatc < 6:361381/148‑1 (MQ=255) cccTGTCGCAGC‑GGGCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCATCTCGATATGCACATCATCGTTGc < 7:255055/147‑1 (MQ=39) cgatctgggCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCACAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTc < 7:176593/143‑1 (MQ=255) ggCTT‑G‑CCAGCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCATATCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGAACGATATGCACATCAACCTTGCCGATGTTc > 8:176593/1‑142 (MQ=255) gCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt > 6:16809/1‑149 (MQ=255) gCTT‑GCCC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATAttt > 3:72258/1‑149 (MQ=255) | ATGGCGGCGTCTTCGAAGAGGATCTGGACATTCGACAGCGGCATTGCAGGTCTCACGAAAATAGCAGTAAACGCTGGGGCCGCTTTGCGGCCCTTCGCGGGCAAGCCCGCTCCTACAAAGGATATGCGTCCCCTGTAGGAGC‑GGGCTT‑G‑CC‑GCGAAGGGCTGCAAGGCAGCCCCAAATTGTCGACTACGCGGATCAGCGATCCGGCAGGGTGATGTTCAGCTCCAGAATCGAGCAGCTGCCCTGGTTTTCCAGCTCGATATGCACATCATCGTTGCCGATGTTCACATATTT > NC_002947/1932490‑1932781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |