Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	1,070,248	+GA	intergenic (+48/+94)	aroP‑I → / ← PP_0928	aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	1,070,246	1	.	G	90.2%	182.5 / 14.6	61	intergenic (+46/+96)	aroP‑I/PP_0928	aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base . (4/2);  new base G (28/27);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NC_002947	1,070,246	2	.	A	90.2%	183.0 / 15.5	61	intergenic (+46/+96)	aroP‑I/PP_0928	aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein
Reads supporting (aligned to +/- strand):  ref base . (4/2);  new base A (28/27);  total (32/29)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC  >  NC_002947/1070098‑1070384                                                                                                                                                        ||                                                                                                                                           ccTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑tgt                                                                                                                                              <  4:74015/149‑1 (MQ=255)  cTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATAGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑tgt                                                                                                                                              >  1:78805/1‑148 (MQ=255)   tGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑Ga                                                                                                                                          >  7:145650/1‑149 (MQ=255)         cgccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑Gagatcga                                                                                                                                    <  8:40263/149‑7 (MQ=255)         cgccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑Gagatc                                                                                                                                      >  3:41085/1‑143 (MQ=255)          gccgATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑Gagatcgag                                                                                                                                   >  5:159468/1‑142 (MQ=255)                  gCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCcgcgc                                                                                                                             >  1:228309/1‑149 (MQ=255)                   cATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCcgcgcg                                                                                                                            >  5:333216/1‑149 (MQ=255)                      ctcGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCgcgggc                                                                                                                         >  5:268902/1‑149 (MQ=255)                       gcGGTACTGCTGATCCCGGCAGGCATCGCTGTGCTGTGGGTGGCTTTCAAGCGGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCgcggg                                                                                                                          <  8:220941/146‑1 (MQ=255)                         ggTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCAAGCGCCGCgcgggcg                                                                                                                        <  5:339827/147‑1 (MQ=255)                               gCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa                                                                                                                >  5:404305/1‑149 (MQ=255)                                    tCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTca                                                                                                           >  4:51725/1‑149 (MQ=255)                                     cccGGCATGGATCGCTGTGCTGTGGGGGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTcac                                                                                                          <  3:30936/149‑1 (MQ=255)                                       cGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg                                                                                                        >  4:301712/1‑149 (MQ=255)                                       cGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAg                                                                                                        >  3:44191/1‑149 (MQ=255)                                              gATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATg                                                                                                  >  8:15084/1‑148 (MQ=255)                                               aTCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGa                                                                                                 >  2:306510/1‑148 (MQ=255)                                                  gctgtgctgtGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc                                                                                             <  6:404305/149‑1 (MQ=255)                                                  gctgtgctgtGGGGGGCTTTCAAGCTGACCAAGGCTCGCCCGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGc                                                                                             <  7:130572/149‑1 (MQ=38)                                                    tgtgctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGcc                                                                                            >  8:230947/1‑148 (MQ=255)                                                        ctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                 >  1:377580/1‑123 (MQ=37)                                                        ctgtgGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCCCGCGCGGCGCTCg                                                                                                                 <  2:377580/123‑1 (MQ=37)                                                        ctgtgGGTGGCTTTCAAGCGGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCCCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaa                                                                                        <  7:358611/148‑1 (MQ=38)                                                          gtgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAgg                                                                                      >  3:31830/1‑148 (MQ=255)                                                           tgggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCa                                                                                    >  6:302019/1‑149 (MQ=255)                                                             ggtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCAt                                                                                   <  5:302019/148‑1 (MQ=255)                                                              gtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaa                                                                                        >  1:157145/1‑142 (MQ=255)                                                              gtggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTaaa                                                                                        <  2:157145/142‑1 (MQ=255)                                                               tggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCg                                                                                >  2:104607/1‑149 (MQ=255)                                                               tggCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCg                                                                                >  5:173654/1‑149 (MQ=255)                                                                         gCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGcc                                                                      <  3:51725/149‑1 (MQ=255)                                                                          cTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGGGCGATATCACAGGCGATGAAAGCCTAAAGGCATGCGTGTGGCGGgcc                                                                     >  2:345069/1‑149 (MQ=38)                                                                            gaagaaGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                                    >  3:278709/1‑148 (MQ=255)                                                                             aagaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCt                                                                    <  6:173654/147‑1 (MQ=255)                                                                                aagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCCCAGGCGATGACAGCCTAAAGGCATGCGTCTGGCGGCCCTGCcgc                                                               >  8:259295/1‑149 (MQ=38)                                                                                 agGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCTTTGA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGAcgc                                                               <  6:333216/148‑1 (MQ=255)                                                                                  ggCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGCGCGCCGCGCGGGCGGCGCGCGGTCGGCCCGGCGCTGCAAGCCGGACGGCCTGGGTCTGGCGGCCCGGAcgcgg                                                             >  2:157108/1‑148 (MQ=255)                                                                                  ggCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGAt                                                                                                   <  3:344437/111‑1 (MQ=255)                                                                                  ggCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGAGACGCGCGGGCGGCGCTCGATCTGACAGGCGAt                                                                                                   >  4:344437/1‑111 (MQ=37)                                                                                   gCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTggcg                                                                         <  5:59004/136‑1 (MQ=255)                                                                                       gCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTccc                                                         <  4:28303/148‑1 (MQ=255)                                                                                             agaaGGGCTGAGCGGGTTTGTTCGTATGGGGTTTGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCGGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCATAAAGGCATGGGTCTGGCGGGGCTGACGCGTTCCCCTTCCAt                                                  <  6:132965/147‑1 (MQ=37)                                                                                               aaGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGc                                                <  6:268902/149‑1 (MQ=255)                                                                                                           ggTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCAGTGA‑‑GATCGAGCGCCGCCCGGGCGGCGCTCGAAATCACAGGCGATGAAAGCCTAAACGCATGCGTCTGGCGGCCCAGACGCGTTACCCATCCATGCCCCTCAAtgcag                                    >  4:184014/1‑147 (MQ=255)                                                                                                                 ttCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCg                              >  2:35682/1‑149 (MQ=255)                                                                                                                 ttCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCg                              <  2:228309/149‑1 (MQ=255)                                                                                                                   cGTAAGGGGTATGCCCTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGACTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATCCTGCCTCCGc                             >  2:253797/1‑148 (MQ=38)                                                                                                                       aGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAg                         <  7:230947/148‑1 (MQ=255)                                                                                                                           gTATGCCGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAgcacg                     >  2:269676/1‑148 (MQ=255)                                                                                                                                 cGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg              <  1:157108/149‑1 (MQ=255)                                                                                                                                 cGTTAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg              <  2:78805/149‑1 (MQ=255)                                                                                                                                   ttAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGATGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt            <  1:104607/149‑1 (MQ=255)                                                                                                                                   ttAGGTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtggt            >  6:353046/1‑149 (MQ=255)                                                                                                                                      ggTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa         <  1:306510/149‑1 (MQ=255)                                                                                                                                       gTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCaa                          <  5:168304/131‑1 (MQ=255)                                                                                                                                       gTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCaa                          >  6:168304/1‑131 (MQ=255)                                                                                                                                       gTTTTCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTa         >  1:9716/1‑148 (MQ=255)                                                                                                                                        ttttCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGGTCGATGTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCGCTGACGCGTTCCCCGTCCATGCCCCTCATTGCTGCCTCCGGCAAGCACGCACAGTGgggtt         >  1:255101/1‑146 (MQ=37)                                                                                                                                        ttttCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACa       <  3:301712/149‑1 (MQ=255)                                                                                                                                           tCGGCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGc     <  4:31830/148‑1 (MQ=255)                                                                                                                                             ggCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCaa   <  7:15084/148‑1 (MQ=255)                                                                                                                                             ggCGCC‑‑TGTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAac  <  2:9716/149‑1 (MQ=255)                                                                                                                                                        ||                                                                                                                                           CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC  >  NC_002947/1070098‑1070384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑