Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_002947	5,555,608	+GCC	intergenic (‑84/+87)	PP_4887 ← / ← PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_002947	= 5555608	4 (0.060)	48 (0.700) +GCC	40/274	1.0	92.5%	intergenic (‑84/+87)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer
	?	NC_002947	5555609 =	4 (0.060)					intergenic (‑85/+86)	PP_4887/PP_4888	hypothetical protein/methyl‑accepting chemotaxis transducer

CAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555474‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCT  >  NC_002947/5555609‑5555753                                                                                                                                         |||                                                                                                                                                    CAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGCAA‑GGGCCGCCAAGCGGCCCCCGCAAATTC                                                                                                                                          >  7:179128/1‑149 CAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATTGCGGACCCGGCAATTTa                                                                                                                                          >  2:288868/1‑148         TCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTA                                                                                                                                  >  2:31797/1‑149           AGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATG                                                                                                                                <  1:383852/149‑1            GGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGC                                                                                                                               <  1:31797/149‑1                                    GGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAG                                                                                                       <  6:366611/149‑1                                             TGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGGGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGC                                                                                            <  1:319905/149‑1                                               AGCAACTCCTTTTGTCATGTGTCGCCGACAGCATTGCAACACCGCTCTGGTGTGGGAGCGGGCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCG                                                                                           <  2:372536/148‑1                                                     TCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTAGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTACGCGGGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑GTTGGCGACACCAG                                                                                    >  5:153181/1‑149                                                       CTTTTGTCATGTGTCGCCGACAGCATCGCACCACCGCTCCGGTGTGGGCGGGGCCTTTGGTCGCTAAC‑GGGCCGCATCGCGGCCCCGGCAATTTCCGCGTGTATCCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAA                                                                                     <  2:329335/146‑1                                                        TTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGG                                                                                                                                                       >  2:338029/1‑81                                                          TTGTCATGTGTCCCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCC                                                                                <  7:208748/148‑1                                                               ATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTACGCGTGTATGCCAAAAACCTGGGGCTGCAATTCACCCC‑‑TTTCGCGACACAACGCCCCCAA                                                                            >  3:259139/1‑147                                                                TGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTCTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAG                                                                          >  1:277258/1‑148                                                                           ctGCATAGCAACCCCGCTCAGGGGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGC                                                              <  8:179128/147‑1                                                                             GCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCTAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTAATCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTT                                                            <  1:288868/149‑1                                                                              CATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCCTAGCGGCCCCGGCAATTTGCGCGTGTATGGAACAAACCTGGGGCTGCTATGCAGCCC‑‑GTTCGCGCCACAAGGCCCCCAAAGGACCGCGCCGGCGTC                                                           >  5:304174/1‑149                                                                                TAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAG                                                         <  1:25430/149‑1                                                                                     ACAGCGCTCAGTTGTGGGAGCGGCGTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCG                                                    <  2:277258/149‑1                                                                                        CCGCTCCGGGGGGGGAGCGGCGTGGTG‑CGCGAAACGGGCCGCATCGGGCCCCCGGCAATTTCCGCGTGTATCCAACAAACCGGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAA                                                 <  5:57739/149‑1                                                                                        CCGCTCAGGTGTGGGAGCGGCCTTGTGTAGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTACGCGTGTATGCAACAAACCTGGGGCTGCTAAGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCCGGCGTCAGGCCCGGA                                                  >  7:313890/1‑148                                                                                         CGCTCTGGTGTGGGAGCGGCCTTGGGCCGCTAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                                <  3:25820/149‑1                                                                                         CGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAAC                                                <  3:31155/149‑1                                                                                              AGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCTGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGG                                             <  8:344075/147‑1                                                                                              AGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACATACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGG                                             <  1:124428/147‑1                                                                                                GTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGC                                                              <  5:147284/128‑1                                                                                                            CCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGCCCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCG                               >  2:138132/1‑147                                                                                                            CCTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCG                               >  4:100110/1‑147                                                                                                             CTTGTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGGGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAG                            >  1:146621/1‑149                                                                                                                GTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGC                           <  6:153181/147‑1                                                                                                                GTGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCAGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCT                          >  8:200898/1‑148                                                                                                                 TGTCGCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGA                        <  3:100110/149‑1                                                                                                                  GTCGCTAAT‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCCTGTTTCGCGACACAAGGCCCACAAATGACCGCTCAGGCTTCAGGCCCGGAACTGGCCCAGGC                                      <  1:318535/136‑1                                                                                                                     GCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAGCTGGCCCAGGCTGGCGCGCAGCT                          <  6:148807/143‑1                                                                                                                     GCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAGCTGGCCCAGGCTGGCGCGCAGCT                          >  5:148807/1‑143                                                                                                                     GCGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCG                    >  1:340654/1‑149                                                                                                                      CGAAA‑GGGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                   >  3:394716/1‑149                                                                                                                       tgccgGGGCCGCTATGCGGCCCTGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                   >  5:357032/6‑149                                                                                                                       ctaatGGGCCTCATAGGGGCCCCGGAAATTTCCGCGTTTATCAAACAAACCTGGGGCTGCTATGCTGCCT‑‑TTTCGCTACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                   <  2:197653/144‑1                                                                                                                        gccgGGGCCGCTATGCGGCCCTGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGC                   >  7:11446/5‑148                                                                                                                             GGCCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGCCACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCGGAGCCGCCAGAT              >  6:25941/1‑148                                                                                                                               CCGCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCAT           >  3:162066/1‑149                                                                                                                                 GCATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCC         <  1:267771/149‑1                                                                                                                                   ATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGG                                       <  2:57624/117‑1                                                                                                                                   ATAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGG                                       >  1:57624/1‑117                                                                                                                                   ATAGCGGCCCCGGCAATTTACGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCTCCAGATCATCCAG       <  6:410/149‑1                                                                                                                                    TAGCGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGC      <  4:62372/149‑1                                                                                                                                       CGGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACC   >  5:23656/1‑149                                                                                                                                        GGCCCCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCT  >  4:328760/1‑149                                                                                                                                         |||                                                                                                                                                    CAGGAAATTCAGGTCGGCCTGGGCAGCGATTAGTCGGGCGGACATGAGCAACTCCTTTTGTCATGTGTCGCCGACAGCATAGCAACACCGCTCAGGTGTGGGAGCGGCCTTGTGTCGCGAAA‑GGGCCGCATAGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_002947/5555474‑5555608 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CCGGCAATTTCCGCGTGTATGCAACAAACCTGGGGCTGCTATGCAGCCC‑‑TTTCGCGACACAAGGCCCACAAAGGACCGCGCAGGCTTCAGGCCCGGAACTGGCCCAGGCTGGCGCGCAGCTGAGCCGCCAGATCATCCAGCACCT  >  NC_002947/5555609‑5555753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.