Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_002947	4,740,816	T→G	intergenic (+53/+700)	gltA → / ← yeiW	citrate synthase/putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	4,740,816	0	T	G	100.0%	131.5 / NA	39	intergenic (+53/+700)	gltA/yeiW	citrate synthase/putative oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/18);  total (21/18)

TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740677‑4740953                                                                                                                                            |                                                                                                                                          tcCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCg                                                                                                                                 >  6:44700/1‑149 (MQ=255)      cccGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCctc                                                                                                                            <  7:29620/149‑1 (MQ=255)               gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                   >  8:1126/1‑149 (MQ=255)               gATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGcc                                                                                                                   <  1:278862/149‑1 (MQ=255)                          cgcgCCAGCTGTACACCGTCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGaa                                                                                                        <  2:386079/149‑1 (MQ=255)                              ccAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAgg                                                                                                    <  1:111526/149‑1 (MQ=255)                               cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc                                                                                                   <  2:296249/149‑1 (MQ=255)                               cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGc                                                                                                   <  5:44700/149‑1 (MQ=255)                               cAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTATTCGCGGCCTTGCCCGCGAAGAGGc                                                                                                   >  5:391895/1‑149 (MQ=255)                                 gCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCACATAGCGGCCGTGACCCCGCAGAGGCCg                                                                                                 >  2:396054/1‑149 (MQ=255)                                    gTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGGCGCATAGCGGCCTTGCCCCGGAAGAGggcggcc                                                                                              >  7:329630/1‑148 (MQ=255)                                       cacCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGca                                                                                              >  1:283359/1‑146 (MQ=255)                                        acCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAACAGGCCGGcaccg                                                                                           >  4:45781/1‑146 (MQ=255)                                                       cgcgACATCGTGCCCCTCAAACCCCCCAAGTAAGCTCCAGTCCCCCACCGCAAGACGGCGGCCCCCGGCCAG‑CTTTTTTTATTTGCCCTGGGCCGCCCTCTGCGCGCCCTTGCCCGCCAAGAGGCCGGCACAGACACCACAAAACCTCa                                                                           <  8:16207/149‑1 (MQ=255)                                                         cgACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCGCAGCCACCACAAAACCTCAGt                                                                         >  5:169730/1‑149 (MQ=255)                                                               cGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCATCGCGGCCTTGCCCGCGAAGAGGCCGGCCCAGACACCACAAAACCAAAGtttttat                                                                   >  5:10020/1‑147 (MQ=255)                                                                      cttaaaTACCGCATGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGACCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                            <  2:283359/146‑1 (MQ=255)                                                                      cTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGTCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccg                                                            >  4:220360/1‑149 (MQ=255)                                                                         aaaGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTccgcccgc                                                           <  5:189605/147‑1 (MQ=255)                                                                            gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACg                                                      >  5:187884/1‑149 (MQ=255)                                                                            gACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGACTTGCCCGCGAAGAGGCCGGCACCGAAACCACAAAACAGCAGTTTTTCTCCGCCCGCTCACg                                                      >  8:306456/1‑149 (MQ=255)                                                                               cGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAg                                                   >  1:277242/1‑149 (MQ=255)                                                                                gCAAGTACGCTGCAGTTGCCGACCGCATGACGGGTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTGCGCGGCCTTGCCCGCGATGAGGCCGGCGCAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGc                                                  <  6:169730/149‑1 (MQ=255)                                                                                 catgTAAGCTGCAGTAGCCTAACGCAATAAGGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGGGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGcc                                                 <  8:125497/146‑1 (MQ=255)                                                                                       cgCTGAAGTAGCCGAACTCAAGAAGGCTGACCTCGGGCAGCC‑TTTTTTATTTGTCCTGGGCCTGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCATTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                            <  7:306456/147‑1 (MQ=255)                                                                                       aGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTc                                            <  6:187884/148‑1 (MQ=255)                                                                                         cTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAgg                                         <  7:177859/149‑1 (MQ=255)                                                                                                 gCCGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACCCCACAAACCCGCAGTTTTTCTCCGCCCGCTCACGCCGCCCGTTCAGGGGATTGaa                                 >  4:60540/1‑149 (MQ=255)                                                                                                  ccGAACGCAAGAAGGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGACCGCGAAGAGGCCGGCACAGACACCACAAAAACTCAGTTTTTCTCAGCCCGCGCCCCCACCCCTTTAAGGGTATTGAAc                                >  6:305286/1‑149 (MQ=255)                                                                                                          aagaagGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTAGCGGCCATGGCCGCGAAGAGGCCCGCACAGACACCCCAAAAACGAAGTTTTTATACGCCCGCGCACGCAGCCCgttaa                                           >  4:41709/1‑128 (MQ=255)                                                                                                          aagaagGCTGCCCTCGGGAAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                           <  3:41709/130‑1 (MQ=255)                                                                                                              agGCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCTAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATccacc                    <  7:1126/149‑1 (MQ=255)                                                                                                                gCTGCCCTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCAGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCcaccac                  >  1:65058/1‑149 (MQ=255)                                                                                                                    cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                           <  5:355371/120‑1 (MQ=39)                                                                                                                    cccTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCa                                           >  6:355371/1‑120 (MQ=39)                                                                                                                      cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTa                                      <  7:360788/123‑1 (MQ=255)                                                                                                                      cTCGGGCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGGTTTTCTCCGCCCCCTCACGCACCCCGTTCAGGGtt                                      >  8:360788/1‑122 (MQ=37)                                                                                                                           gCAGCC‑TTTTTTATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGCGGCCGGCACAGGCCCCACACAACCTCAGGTTTTGTGCGCCCGCGCACGCCGCCCGTTGAGGGTATTGAACGGGGCAGCCCCCACGGAGGTGTTgg       >  4:102637/1‑149 (MQ=255)                                                                                                                                  ttttttATTTGTCCTGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGGACAGACACCCCAAAACCTCAGTTTTTATCCGCCCGCGCCAGCAGCCCTTTCAGGGGTTTGAACGGCGCATCCCCCACGCACATGTTTGccccc  >  8:275848/1‑148 (MQ=255)                                                                                                                                            |                                                                                                                                          TCCAGCCCGTACAAGATCGGCCGTCCGCGCCAGCTGTACACCGGCGAGCAGAAGCGCGACATCGTTGCCCTGAAAGACCGCAAGTAAGCTGCAGTAGCCGAACGCAAGAAGGCTGCCCTCGGGCAGCCTTTTTTTATTTTTCTGGTGCCGGCCTCTTCGCGGCCTTGCCCGCGAAGAGGCCGGCACAGACACCACAAAACCTCAGTTTTTCTCCGCCCGCTCACGCAGCCCTTTCAGGGTATTGAACGGCGCATCCACCACGAACTTGTTGGCCACC  >  NC_002947/4740677‑4740953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑