| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 499,203 | A→G | intergenic (+47/‑249) | uhpA → / → PP_0411 | two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 499,203 | 0 | A | G | 98.1% | 176.8 / NA | 52 | intergenic (+47/‑249) | uhpA/PP_0411 | two‑component system response regulator UhpA/polyamine ABC transporter ATP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (22/29); minor base C (1/0); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC > NC_002947/499058‑499344 | aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑c < 8:95953/149‑1 (MQ=255)aaGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCCggg > 1:196081/1‑148 (MQ=255) cATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGcc < 4:286741/149‑1 (MQ=255) tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCcg < 3:40011/149‑1 (MQ=255) tCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCcg > 3:242768/1‑149 (MQ=255) gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCcgc > 1:132726/1‑147 (MQ=255) gCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCcgc < 2:132726/147‑1 (MQ=255) cACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGaa < 8:6004/149‑1 (MQ=255) aCCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAAc > 4:299345/1‑149 (MQ=255) cccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACg > 6:381989/1‑149 (MQ=255) ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACgg > 2:293497/1‑149 (MQ=255) gtgggtgcTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGggcct < 7:29774/140‑1 (MQ=255) ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGggccggg > 8:4125/1‑149 (MQ=255) gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CAt > 1:363434/1‑130 (MQ=255) gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CAt < 2:363434/130‑1 (MQ=255) cACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCTGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGggcctggcc > 7:144148/1‑149 (MQ=255) gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCTGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCctgc < 8:144148/149‑1 (MQ=255) cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCctgctg < 6:195749/149‑1 (MQ=255) cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCctgctg < 1:62520/149‑1 (MQ=255) gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGcc > 7:114184/1‑125 (MQ=255) gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGcc < 8:114184/125‑1 (MQ=255) tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGAt < 5:155048/147‑1 (MQ=255) tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGAt > 6:155048/1‑147 (MQ=255) gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc < 5:381989/149‑1 (MQ=255) gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGc < 8:374995/149‑1 (MQ=255) gTGAACTCGCTGGCGGAGCGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCg < 6:262636/149‑1 (MQ=255) gAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg < 1:293497/149‑1 (MQ=255) aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACgg < 4:250603/115‑1 (MQ=255) aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACgg > 3:250603/1‑115 (MQ=255) aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGcc > 5:192795/1‑149 (MQ=255) cTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATTCGGGTGCGCGAAAGTTGCCTAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGggcctg < 3:232724/114‑1 (MQ=255) gCGGAGCTGGTGAAGTACGCCATGTAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGAt > 6:390285/1‑121 (MQ=255) gCGGAGCTGGTGAAGTACGCCATGTAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGAt < 5:390285/121‑1 (MQ=255) ggAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGc < 3:20821/149‑1 (MQ=255) gAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAATGCTGAGCCGGCCTCTTCGCGGGCa > 6:162386/1‑149 (MQ=255) aGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCAt < 3:299345/149‑1 (MQ=255) cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc < 6:192795/149‑1 (MQ=255) gTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACgg < 3:357417/92‑1 (MQ=255) gTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACgg > 4:357417/1‑92 (MQ=255) cGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGCGCCGGCCTCTTCGCGGGCATGCCCGCTcccc > 7:3596/1‑146 (MQ=255) cATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATTCCCGCGGACCGGCCTGGGCTGCTGCTAAGGATTGGTGGGGCGGCCTCATAGCGGGCAGTGCCGCTCCCACAAGt > 7:74092/1‑149 (MQ=255) aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGt < 5:293337/149‑1 (MQ=255) cacaAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTGCCCCAAGCCTATGTGGGAGCGGG‑CTTGCCCGCGAACGGGCCGGGCCTGCTTACCTCTATGGCTGAGCCGGCCTCTGCGCGGGCATGCCCGCTCCCACAGGTTTCGTgg < 2:241182/149‑1 (MQ=38) gatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CAAGCCCCCGCACCGGCCAGGGCGGGGGATGTAGAATGCGGAGGCGGGCAATTAGCCGGCCAGCCCCCGGCCACAGGTTTGGTGGTTGGcgcg > 2:342091/1‑149 (MQ=255) atctgatTGCGCAATACGGGTGCCCGAACCTTGCCGAAGCCTATGTGGGAGCGGG‑CAGGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGt < 5:131065/138‑1 (MQ=38) atctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGGACGGGCCTGGCCTGCTGATCTCTATGGCGGAGCCGGCCTATTGGCGGGCATGCCCGCTCCCCCAGGTTTCGt > 6:131065/1‑138 (MQ=255) gatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg > 3:197622/1‑109 (MQ=255) gatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCgg < 4:197622/109‑1 (MQ=255) aCTTGCCGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCGGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGt > 5:351721/1‑149 (MQ=255) ccGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGAtct < 6:410815/54‑1 (MQ=255) ccGAAGCCTATGTGGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGAtct > 5:410815/1‑54 (MQ=255) tgGGAGCGGG‑CATGCCCGCGAACGGGCCTGGCCTGCTCATCTCTATGGCTGACCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGATCGCCCTacaag < 7:4125/149‑2 (MQ=38) | AAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGG‑CATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAAC > NC_002947/499058‑499344 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |